HINT1 antibody
Principal name
HINT1 antibody
Alternative names for HINT1 antibody
HINT1, HINT, PKCI1, PRKCNH1, Histidine triad nucleotide-binding protein 1, Adenosine 5'-monophosphoramidase, Protein kinase C inhibitor 1, Protein kinase C-interacting protein 1, PKCI-1
SwissProt ID
P49773 (Human), P62958 (Bovin), P62959 (Rat), P70349 (Mouse), P80912 (Rabit), Q5RF69 (Ponab)
Ncbi ID
9606, NP_005331, NP_005331.1, NM_005340, NP_032274.1, NM_008248, NM_001109607
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of HINT1 antibody
- Kit Component:
- - KN307718G1, Hint1 gRNA vector 1 in pCas-Guide vector
- KN307718G2, Hint1 gRNA vector 2 in pCas-Guide vector
- KN307718D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Zhang YJ. et al.(2009), Cancer Lett, 275(2):277-84.
Pawel Bieganowski. et al. (2002), J Biol Chem. 277(13):10852–10860.
Recombinant human HINT1, 1-126 aa: 15% SDS-PAGE (3 µg)
Immunoperoxidase of monoclonal antibody to HINT1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]
Western Blot analysis of HINT1 expression in transfected 293T cell line by HINT1 monoclonal antibody (M05), clone 2D7.Lane 1: HINT1 transfected lysate(13.8 KDa).Lane 2: Non-transfected lysate.
Human Kidney; Kidney; HINT1 antibody - N-terminal region (ARP54766_P050) in Human Kidney cells using Immunohistochemistry
Human Jurkat; WB Suggested Anti-HINT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. HINT1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells.; HINT1 antibody - N-terminal region (ARP54766_P050) in Human Jurkat cells using Western Blot
Human Kidney; Kidney; HINT1 antibody - N-terminal region (ARP54767_P050) in Human Kidney cells using Immunohistochemistry
Human Jurkat; Host:Rabbit. Target Name:HINT1. Sample Tissue:Human Jurkat. Antibody Dilution: 1.0ug/ml. HINT1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat.; HINT1 antibody - N-terminal region (ARP54767_P050) in Human Jurkat cells using Western Blot
Human 721_B; WB Suggested Anti-HINT1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. HINT1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; HINT1 antibody - N-terminal region (ARP54767_P050) in Human 721_B cells using Western Blot
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 µg/ml.
Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/mL.
Western blot analysis of extracts from Jurkat cells, using HINT1 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using HINT1 antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-HINT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysateHINT1 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Kidney
Host: Rabbit; Target Name: HINT1; Sample Tissue: Human Jurkat; Antibody Dilution: 1.0 ug/mlHINT1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat
WB Suggested Anti-HINT1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysateHINT1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Kidney
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306652 | HINT1 antibody |
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| Rabbit Polyclonal HINT1 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA312903 | HINT1 antibody |
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| Rabbit polyclonal anti-HINT1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA351246 | HINT1 antibody |
||||||
| Rabbit Polyclonal Anti-HINT1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP23860PU-N | HINT1 antibody |
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 |
Rabbit | Purified | Hu, Ms, Rt | P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303319 | HINT1 (transcript variant 1) |
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| Recombinant protein of human histidine triad nucleotide binding protein 1 (HINT1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417372 | HINT1 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200661L2V | Lenti ORF particles, Hint1 (GFP-tagged) - Mouse histidine triad nucleotide binding protein 1 (Hint1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR200661L1V | Lenti ORF particles, Hint1 (Myc-DDK-tagged) - Mouse histidine triad nucleotide binding protein 1 (Hint1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR201830L2V | Lenti ORF particles, Hint1 (GFP-tagged ORF) - Rat histidine triad nucleotide binding protein 1 (Hint1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR201830L1V | Lenti ORF particles, Hint1 (Myc-DDK-tagged ORF) - Rat histidine triad nucleotide binding protein 1 (Hint1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||