HINT2 antibody
Principal name
HINT2 antibody
Alternative names for HINT2 antibody
HINT-2, HINT-3, HIT-17kDa, PKCI-1-related HIT protein, Histidine triad nucleotide-binding protein 2
Ncbi ID
9606, NM_032593, NP_115982, NP_115982.1, NP_081147.1, NM_026871
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F)
Background of HINT2 antibody
- Kit Component:
- - KN307719G1, Hint2 gRNA vector 1 in pCas-Guide vector
- KN307719G2, Hint2 gRNA vector 2 in pCas-Guide vector
- KN307719D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Maize KM., et al. (2013) The FEBS journal. 280(14): 3389-3398.Lenglet S., et al. (2008) Endocrinology. 149(11): 5461-5469.Martin J., et al. (2006) Gastroenterology. 130(7): 2179-2188.Lee LR., et al. (2013) Cancer prevention research. 6(7): 731-743.
Human Brain; WB Suggested Anti-HINT2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human brain; HINT2 antibody - C-terminal region (ARP60265_P050) in Human Brain cells using Western Blot
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using HINT2 antibody at dilution 1/20, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human breast and colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast and colon cancer tissue using HINT2 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human breast and colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast and colon cancer tissue using HINT2 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200)
WB Suggested Anti-HINT2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain
The recombinant human HINT2 (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HINT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HINT2 Recombinant proteinLane 2. : HepG2 cell lysate Lane 3. : U87MG cell lysate Lane 4. : Mouse brain lysate
Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, human hepatocellular carcinoma tissue, Primary antibody: (HINT2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (HINT2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (HINT2 Antibody) at dilution 1/50, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321918 | HINT2 antibody |
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| Anti-HINT2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321919 | HINT2 antibody |
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| Anti-HINT2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA349338 | HINT2 antibody |
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| Rabbit Polyclonal Anti-HINT2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM50638PU-N | HINT2 antibody |
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 |
Mouse | IgG1 | AT5B10 | Purified | Hu | E, F, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| AM50638PU-S | HINT2 antibody |
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|
Mouse | IgG1 | AT5B10 | Purified | Hu | E, F, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP308371 | HINT2 |
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| Recombinant protein of human histidine triad nucleotide binding protein 2 (HINT2) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403175 | HINT2 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR220622L2V | Lenti ORF particles, Hint2 (GFP-tagged) - Mouse histidine triad nucleotide binding protein 2 (Hint2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR220622L1V | Lenti ORF particles, Hint2 (Myc-DDK-tagged) - Mouse histidine triad nucleotide binding protein 2 (Hint2), nuclear gene encoding mitochondrial protein, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||