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HIP2 / UBE2K antibody

Principal name

HIP2 / UBE2K antibody

Alternative names for HIP2 / UBE2K antibody

HIP-2, Ubiquitin-conjugating enzyme E2 K, E2(25K), E2-25K, Ubiquitin-protein ligase, LIG, Ubiquitin carrier protein, Huntingtin-interacting protein 2

SwissProt ID

P61085 (Bovin), P61086 (Human), P61087 (Mouse)

Gene ID

3093 (UBE2K), 53323 (Ube2k)

Ncbi ID

NP_001104583.1, 9606, NP_005330, NP_001104583, NM_001111113, NP_005330.1, NP_058066.2, NM_005339

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Por (Porcine), Rt (Rat), Ze (Zebrafish), African clawed frog, Bt (Bat), Eq (Equine), Fi (Fish), Gt (Goat), Hst (Hamster), Mky (Monkey), Op (Opossum), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), Bear

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of HIP2 / UBE2K antibody

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene.
HIP2 belongs to the ubiquitin conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid bearing macrophages through ubiquitination and subsequent degradation of p53.

General readings

1. Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR.
Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme. J Biol Chem. 1996 Aug 9;271(32):19385-94. PMID: 8702625

UBE2K polyclonal antibody ( Cat # PAB6301 ) staining ( 1.5 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

PAB6301

Human Jurkat; WB Suggested Anti-HIP2 Antibody Titration: 5.0ug/ml. Positive Control: Jurkat cell lysate; HIP2 antibody - N-terminal region (ARP43048_T100) in Human Jurkat cells using Western Blot

ARP43048_T100

Human HepG2; WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; UBE2K antibody - N-terminal region (ARP58011_P050) in Human HepG2 cells using Western Blot

ARP58011_P050

Human Liver; WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Liver; UBE2K antibody - middle region (ARP58012_P050) in Human Liver cells using Western Blot

ARP58012_P050

Transfected 293T; WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; UBE2K antibody - middle region (ARP43049_P050) in Transfected 293T cells using Western Blot

ARP43049_P050

Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23819PU-N

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP23819PU-N

Antibody staining (1.5 µg/ml) of Jurkat lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

AP23819PU-N

Recombinant human UBE2K, 1-200 aa, His-tagged

TP308645

TP720163

TA302847 staining (1.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

TA302847

Predicted band size: 22 kDa . Positive control: Human brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

TA323779

Western blot analysis of lysates from Hela, 293 cell line, mouse brain tissue lysate, C2C12 cell line (from left to right), using UBE2K Antibody (N-term)(Cat. #TA324679). TA324679 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

TA324679

WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Liver

TA344492

WB Suggested Anti-UBE2K Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate

TA344493

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
TA302847	HIP2 / UBE2K (C-term) antibody
	Goat Polyclonal Antibody against HIP2
	Goat		Aff - Purified	Bov, Can, Hu, Ms, Por	WB	0.1 mg / €360.00
						OriGene Technologies, Inc.
TA323779	HIP2 / UBE2K antibody
	Anti-UBE2K Rabbit Polyclonal Antibody
	Rabbit	IgG	Purified	Hu, Ms, Rt	WB	0.1 ml / €335.00
						OriGene Technologies, Inc.
TA324679	HIP2 / UBE2K antibody
	Rabbit polyclonal UBE2K Antibody(N-term)
	Rabbit	IgG	Purified	Bov, Hu, Ms	WB	0.4 ml / €415.00
						OriGene Technologies, Inc.
AP23819PU-N	HIP2 / UBE2K (144-157) antibody
	Goat		Aff - Purified	Bov, Bt, Can, Chk, Eq, Gt, Hst, Hu, Mky, Ms, Por, Rb, Rt, Xen, Ze	E, P, WB	50 µg / €425.00
						OriGene Technologies GmbH
+2 additional images

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP308645	HIP2 / UBE2K (transcript variant 1)
	Recombinant protein of human ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) (UBE2K), transcript variant 1
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP720163	HIP2 / UBE2K (transcript variant 3)
	Recombinant protein of human ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast) (UBE2K), transcript variant 3
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	10 µg / €299.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY426354	UBE2K overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

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