HIPK2 antibody
Principal name
HIPK2 antibody
Alternative names for HIPK2 antibody
Homeodomain-interacting protein kinase 2
Ncbi ID
NP_073577, 9606, NP_001106710, AAI68360.1, NP_001106710.1, NM_022740, NP_001129537.1, BC031904, NM_010433, NM_001113239
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E)
Background of HIPK2 antibody
- Kit Component:
- - KN307724G1, Hipk2 gRNA vector 1 in pCas-Guide vector
- KN307724G2, Hipk2 gRNA vector 2 in pCas-Guide vector
- KN307724D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Kim,E.A., (2006) J. Biol. Chem. 281 (11), 7489-7497
Human Heart; Rabbit Anti-HIPK2 Antibody. Catalog Number: ARP32586. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; HIPK2 Antibody - middle region (ARP32586_P050) in Human Heart cells using Immunohistochemistry
Human Pineal Tissue; Rabbit Anti-HIPK2 Antibody . Catalog Number: ARP32586_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Nuclear in pinealocytes & intersticial cells . Primary Antibody Concentration: 1:100 . Other Working Concentrations: 1/600 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-HIPK2 Antibody (ARP32586_P050) ) in Human Pineal Tissue using Immunohistochemistry
Human Heart; . Rabbit Anti-HIPK2 Antibody. . Catalog Number:. ARP32586 . Paraffin Embedded Tissue: Human cardiac cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Human Brain; . Rabbit Anti-HIPK2 Antibody. . Catalog Number:. ARP32586 . Paraffin Embedded Tissue: Human neural cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
HepG2; Host: Rabbit. Target Name:HIPK2. Sample Tissue:HepG2. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:1.0ug/mL. Peptide Concentration: 1.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 6%-18%. HIPK2 is supported by BioGPS gene expression data to be expressed in HepG2.; HIPK2 Antibody - middle region (ARP32586_P050) in HepG2 cells using Western Blot
Human Jurkat; WB Suggested Anti-HIPK2 Antibody Titration: 0.1ug/ml. Positive Control: Jurkat Cell Lysate; HIPK2 Antibody - middle region (ARP32586_P050) in Human Jurkat cells using Western Blot
Human HepG2; WB Suggested Anti-HIPK2 Antibody Titration: 0.06ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate. HIPK2 is supported by BioGPS gene expression data to be expressed in HepG2.; HIPK2 Antibody - middle region (ARP32586_P050) in Human HepG2 cells using Western Blot
HIPK2 monoclonal antibody (M01), clone 4F4. Western Blot analysis of HIPK2 expression in human ovary cancer.
HIPK2 monoclonal antibody (M01), clone 4F4. Western Blot analysis of HIPK2 expression in H9c2(2-1).
Immunoperoxidase of monoclonal antibody to HIPK2 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 1 ug/ml]
HIPK2 monoclonal antibody (M03), clone 1F10. Western Blot analysis of HIPK2 expression in RIN-m5F.
HIPK2 monoclonal antibody (M03), clone 1F10 Western Blot analysis of HIPK2 expression in MES-SA/Dx5 ( Cat # L021V1 ).
Detection limit for recombinant GST tagged HIPK2 is approximately 0.1ng/ml as a capture antibody.
HIPK2 monoclonal antibody (M06), clone 4E3. Western Blot analysis of HIPK2 expression in human ovary cancer.
HIPK2 monoclonal antibody (M06), clone 4E3. Western Blot analysis of HIPK2 expression in RIN-m5F.
Dot blot analysis of anti-Phospho-HIPK2-pY361 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIPK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-HIPK2 Antibody (N-term) in Ramos cell line lysates (35ug/lane). HIPK2(arrow) was detected using the purified Pab (1:200 dilution).
Western blot analysis of anti-HIPK2 Antibody in NCI-H460 cell line lysates (35ug/lane). HIPK2 (arrow) was detected using the purified Pab.
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of HIPK2 expression in RIN-m5F cells with HIPK2 monoclonal antibody, clone 1F10.
Western Blot analysis of HIPK2 expression in MES-SA/Dx5 cells with HIPK2 monoclonal antibody, clone 1F10.
Detection limit for recombinant GST tagged HIPK2 is approximately 0.1ng/ml as a capture antibody.
Western blot analysis of HIPK2 expression in HepG2 (A); MCF7 (B); SP2/0 (C); PC12 (D) whole cell lysates.
WB Suggested Anti-HIPK2 Antibody Titration: 0.1ug/ml; Positive Control: Jurkat Cell Lysate
WB Suggested Anti-HIPK2 Antibody Titration: 0.06ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysateHIPK2 is supported by BioGPS gene expression data to be expressed in HepG2
Host: Rabbit; Target Name:HIPK2; Sample Tissue:HepG2; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:1.0ug/mL; Peptide Concentration: 1.0ug/mL; Lysate Quantity: 25ug/lane; Gel Concentration: 6%-18%HI
Rabbit Anti-HIPK2 Antibody; Paraffin Embedded Tissue: Human Heart; Cellular Data: Myocardial cells; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Rabbit Anti-HIPK2 Antibody; Paraffin Embedded Tissue: Human cardiac cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Rabbit Anti-HIPK2 Antibody; Paraffin Embedded Tissue: Human neural cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Rabbit Anti-HIPK2 Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue; Observed Staining: Nuclear in pinealocytes & intersticial cells; Primary Antibody Concentration: 1:100; Other Working Concentrations: 1/600; Secondary Antibody: Don
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AM31106PU-N | HIPK2 antibody |
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Mouse | IgG2a | 1F10 | Purified | Hu, Rt | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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| AP13026PU-N | HIPK2 pTyr361 antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP14175PU-N | HIPK2 antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN220278 | HIPK2 - human gene knockout kit via CRISPR |
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 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN220278BN | HIPK2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN220278LP | HIPK2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN220278RB | HIPK2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP320278 | HIPK2 (transcript variant 1) |
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| Recombinant protein of human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€788.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY411573 | HIPK2 overexpression lysate |
|
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0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC220278L2V | Lenti ORF particles, HIPK2 (mGFP-tagged) - Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,500.00
|
|
| OriGene Technologies, Inc. | ||
| RC226348L2V | Lenti ORF particles, HIPK2 (mGFP-tagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,460.00
|
|
| OriGene Technologies, Inc. | ||
| RC220278L1V | Lenti ORF particles, HIPK2 (Myc-DDK tagged) - Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 1 , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,260.00
|
|
| OriGene Technologies, Inc. | ||
| RC226348L1V | Lenti ORF particles, HIPK2 (Myc-DDK-tagged)-Human homeodomain interacting protein kinase 2 (HIPK2), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€2,220.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR211742L2V | Lenti ORF particles, Hipk2 (GFP-tagged) - Mouse homeodomain interacting protein kinase 2 (cDNA clone MGC:36065 IMAGE:5368577), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,890.00
|
|
| OriGene Technologies, Inc. | ||
| MR211742L1V | Lenti ORF particles, Hipk2 (Myc-DDK-tagged) - Mouse homeodomain interacting protein kinase 2 (cDNA clone MGC:36065 IMAGE:5368577), 200 uL, >10^7 TU/mL |
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|
200 µl /
€1,700.00
|
|
| OriGene Technologies, Inc. | ||