HKR1 antibody
Principal name
HKR1 antibody
Alternative names for HKR1 antibody
Krueppel-related zinc finger protein 1
SwissProt ID
P10072 (Human)
Gene ID
284459 (HKR1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of HKR1 antibody
HKR1 is one of the GLI-Kruppel family of human genes. Members of this family have similar H-C links, i.e., a conserved stretch of 9 amino acids connecting the C-terminal histidine of one finger to the N-terminal cysteine of the next. On the basis of amino acid sequence and intron-exon organization, the genes could be placed into one of two subgroups: the GLI subgroup (with the consensus finger amino acid sequence [Y/F]XCX3GCX3[F/Y]X5LX2HX3-4H[T/S]GEKP) or the Kr subgroup (with the consensus finger amino acid sequence [Y/F]XCX2CX3FX5LX2HXRXHTGEKP).
General readings
Suzuki,Y., et al., Gene 200 (1-2), 149-156 (1997)
Human Jurkat; WB Suggested Anti-HKR1 Antibody Titration: 0.3125ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; HKR1 antibody - N-terminal region (ARP36259_T100) in Human Jurkat cells using Western Blot
Human Heart; HKR1 antibody - C-terminal region (ARP40041_P050) in Human Heart cells using Immunohistochemistry
Human kidney; HKR1 antibody - C-terminal region (ARP40041_P050) in Human kidney cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-HKR1 Antibody Titration: 0.125ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; HKR1 antibody - C-terminal region (ARP40041_P050) in Human HepG2 cells using Western Blot
Human Placenta; WB Suggested Anti-HKR1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; HKR1 antibody - N-terminal region (ARP40040_P050) in Human Placenta cells using Western Blot
Immunohistochemistry-Paraffin: HKR1 Antibody [NBP1-86826] - Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in seminiferus ducts.
Western Blot: HKR1 Antibody [NBP1-86826] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunofluorescence analysis of HuvEc cells, using HKR1 Antibody. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa/Jurkat/COLO205 cells, using HKR1 Antibody. The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of HKR1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of HKR1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of HKR1 expression in HepG2 (A); NIH3T3 (B); mouse liver (C); rat liver (D) whole cell lysates.
COLO 320 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP( HKR1 Antibody) at dilution of 1:300
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14902-1-AP( HKR1 Antibody) at dilution of 1:300
HeLa cells were subjected to SDS PAGE followed by western blot with 14902-1-AP( HKR1 Antibody) at dilution of 1:300
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA316317 | HKR1 antibody |
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| Rabbit polyclonal anti-HKR1 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€335.00
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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