HMG20A antibody
Principal name
HMG20A antibody
Alternative names for HMG20A antibody
HMGX1, HMGXB1, High mobility group protein 20A, HMG box-containing protein 20A, HMG domain-containing protein HMGX1, HMG domain-containing protein 1
Gene ID
10363 (HMG20A)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection))
Background of HMG20A antibody
- Kit Component:
- - KN201894G1, HMG20A gRNA vector 1 in pCas-Guide vector
- KN201894G2, HMG20A gRNA vector 2 in pCas-Guide vector
- KN201894D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Rual,J.F., et al., (2005) Nature 437 (7062), 1173-1178
Human Hela, 293T; WB Suggested Anti-HMG20A Antibody. . Titration: 1.25 ug/ml. . Positive Control: Hela/293T; HMG20A antibody - middle region (ARP32759_T100) in Human Hela, 293T cells using Western Blot
Human 293T; WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate. HMG20A is supported by BioGPS gene expression data to be expressed in HEK293T.; HMG20A antibody - middle region (ARP32759_P050) in Human 293T cells using Western Blot
Human Jurkat; WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; HMG20A antibody - C-terminal region (ARP39340_P050) in Human Jurkat cells using Western Blot
Western Blot analysis of HMG20A expression in transfected 293T cell line by HMG20A monoclonal antibody (M01), clone 4D5.Lane 1: HMG20A transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.
Spleen: Formalin-Fixed Paraffin-Embedded (FFPE)
Transfected 293T; WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; HMG20A antibody - N-terminal region (ARP39339_P050) in Transfected 293T cells using Western Blot
Human 293T; WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. HMG20A is supported by BioGPS gene expression data to be expressed in HEK293T.; HMG20A antibody - N-terminal region (ARP31471_P050) in Human 293T cells using Western Blot
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807000)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMG20A (RC201894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMG20A.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMG20A (RC201894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMG20A.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807041)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807041)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMG20A (RC201894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMG20A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807042)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMG20A (RC201894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMG20A.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807044)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807044)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HMG20A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807044)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HMG20A (RC201894, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HMG20A.
TA305740 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateHMG20A is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-HMG20A Antibody; Titration: 1.25 ug/ml; Positive Control: Hela/293T
WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T
WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Jurkat cell lysate
WB Suggested Anti-HMG20A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: 293T cell lysateHMG20A is supported by BioGPS gene expression data to be expressed in HEK293T
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA807042 | HMG20A antibody |
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| HMG20A mouse monoclonal antibody, clone OTI2H4 (formerly 2H4) | |||||||
 |
Mouse | IgG1 | OTI2H4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +17 additional images |
Highly validated antibody!
|
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| TA807044 | HMG20A antibody |
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| HMG20A mouse monoclonal antibody, clone OTI1C2 (formerly 1C2) | |||||||
 |
Mouse | IgG2a | OTI1C2 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA305740 | HMG20A (C-term) antibody |
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| Goat Anti-HMG20A Antibody | |||||||
 |
Goat | Aff - Purified | Bov, Can, Hu, Ms, Por, Rt | E, P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA807000 | HMG20A antibody |
||||||
| HMG20A mouse monoclonal antibody, clone OTI5E4 (formerly 5E4) | |||||||
 |
Mouse | IgG1 | OTI5E4 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA807001 | HMG20A antibody |
||||||
| HMG20A mouse monoclonal antibody, clone OTI5G3 (formerly 5G3) | |||||||
 |
Mouse | IgG2a | OTI5G3 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA807041 | HMG20A antibody |
||||||
| HMG20A mouse monoclonal antibody, clone OTI5E3 (formerly 5E3) | |||||||
 |
Mouse | IgG1 | OTI5E3 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP08833PU-N | HMG20A (336-347) antibody |
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 |
Goat | IgG | Aff - Purified | Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Platypus, Por, Rb, Rt | E, P |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301894 | HMG20A |
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| Recombinant protein of human high-mobility group 20A (HMG20A) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY413245 | HMG20A overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
