HPGD antibody
Principal name
HPGD antibody
Alternative names for HPGD antibody
15-hydroxyprostaglandin dehydrogenase, PGDH, PGDH1, Prostaglandin dehydrogenase 1
Ncbi ID
9606, NP_000851, NM_000860, NP_000851.2, NM_001145816, NM_008278, NM_024390, NP_032304.2
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Luminex (LMNX)
Background of HPGD antibody
- Kit Component:
- - KN307923G1, Hpgd gRNA vector 1 in pCas-Guide vector
- KN307923G2, Hpgd gRNA vector 2 in pCas-Guide vector
- KN307923D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Coggins KG, Latour A, Nguyen MS, Audoly L, Coffman TM, Koller BH. Metabolism of PGE2 by prostaglandin dehydrogenase is essential for remodeling the ductus arteriosus. Nat Med. 2002 Feb;8(2):91-2.
HPGD MaxPab polyclonal antibody. Western Blot analysis of HPGD expression in human liver.
Western Blot analysis of HPGD expression in transfected 293T cell line (H00003248-T01) by HPGD MaxPab polyclonal antibody.Lane 1: HPGD transfected lysate(29.37 KDa).Lane 2: Non-transfected lysate.
HPGD polyclonal antibody ( Cat # PAB6712 ) ( 0.1 µg/ml ) staining of human Duodenum lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
HPGD MaxPab polyclonal antibody. Western Blot analysis of HPGD expression in human liver.
Western Blot analysis of HPGD expression in transfected 293T cell line (H00003248-T01) by HPGD MaxPab polyclonal antibody.Lane 1: HPGD transfected lysate(29.37 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HPGD expression in transfected 293T cell line (H00003248-T01) by HPGD MaxPab polyclonal antibody.Lane 1: HPGD transfected lysate(29.37 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of HPGD in LoVo whole cell lysates using HPGD polyclonal antibody ( Cat # PAB12548 ). Lane 1 uses a 1 : 6000 dilution. Lane 2 uses a 1 : 1500 dilution.
Western Blot analysis of HPGD expression in transfected 293T cell line (H00003248-T03) by HPGD MaxPab polyclonal antibody.Lane 1: HPGD transfected lysate(29.00 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of HPGD Antibody (C-term) (AP17482PU-N) in MDA-MB231 cell line lysates (35ug/lane). HPGD (arrow) was detected using the purified Pab.
Recombinant human HPGD, 1-266 aa, His-tagged
Human Colon (formalin-fixed, paraffin-embedded) stained with HPGD antibody AP22571PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Liver (formalin-fixed, paraffin-embedded) stained with HPGD antibody AP22571PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
(0.1 µg/ml) staining of Human Duodenum lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Human MDA-MB-435S; WB Suggested Anti-HPGD Antibody. . Titration: 1.0 ug/ml. . Positive Control: MDA-MB-435S Whole Cell; HPGD antibody - N-terminal region (ARP61011_P050) in Human MDA-MB-435S cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502450)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD.
HEK293T cells transfected with either RC204160 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HPGD antibody(TA502450), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD.
HEK293T cells transfected with either RC204160 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HPGD antibody(TA502451), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502452)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502452)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502452)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD.
HEK293T cells transfected with either RC204160 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HPGD antibody(TA502452), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502484)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502484)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502484)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502484)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HPGD monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells transfected with either RC204160 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HPGD antibody(TA502484), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502561)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502561)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD.
Flow cytometric Analysis of Jurkat cells, using anti-HPGD antibody(TA502561),(Red), compared to a nonspecific negative control antibody,(Blue).
HEK293T cells transfected with either RC204160 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HPGD antibody(TA502561), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-HPGD antibody(TA502561),(Red), compared to a nonspecific negative control antibody,(Blue).
HPGD ELISA with 1C10 Capture (TA600099) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 1C10 Capture (TA600099) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 1C12 Capture (TA600100) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 1C12 Capture (TA600100) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 2C10 Capture (TA600101) and 1C10 Detection (TA700099) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 2C10 Capture (TA600101) and 1E9 Detection (TA700100) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 2C10 Capture (TA600101) and 1C10 Detection (TA700099) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 2C10 Capture (TA600101) and 1E9 Detection (TA700100) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 2F5 Capture (TA600102) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 2F5 Capture (TA600102) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 2C10 Capture (TA600101) and 1C10 Detection (TA700099) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 2C10 Capture (TA600101) and 1C10 Detection (TA700099) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 2C10 Capture (TA600101) and 1E9 Detection (TA700100) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 2C10 Capture (TA600101) and 1E9 Detection (TA700100) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 1C10 Capture (TA600099) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 1C12 Capture (TA600100) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD ELISA with 2F5 Capture (TA600102) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 1C12 Capture (TA600100) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 2F5 Capture (TA600102) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
HPGD Luminex with 1C10 Capture (TA600099) and 2C10 Detection (TA700101) Antibodies. Substrate used: Recombinant Human HPGD (TP304160)
Detection of 15-PGDH in LoVo whole cell lysates. Lane 1 uses a 1:6,000 dilution. Lane 2 uses a 1:1,500 dilution. ECL: 10 second exposure.
Immunohistochemistry-Paraffin: IHC analysis of 15-PGDH in human synovial tissue from patient with rheumatoid arthritis .
Immunocytochemistry/Immunofluorescence: 15-PGDH antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
TA302977 (0.1µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Sample (30ug whole cell lysate). A:Raji . 12% SDS PAGE. TA308509 diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using 15-PGDH(TA308509) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 15-PGDH(TA308509) antibody at 1:200 dilution.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA301413 | HPGD antibody |
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| Rabbit Polyclonal Antibody against 15-PGDH | |||||||
 |
Rabbit | IgG | Purified | Hu | ICC/IF, WB |
0.1 ml /
€450.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA302977 | HPGD (C-term) antibody |
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| Goat Polyclonal Antibody against HPGD | |||||||
 |
Goat | Aff - Purified | Hu | E, P, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA308509 | HPGD antibody |
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| Rabbit Polyclonal antibody to 15-PGDH (hydroxyprostaglandin dehydrogenase 15-(NAD)) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP17482PU-N | HPGD (C-term) antibody |
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 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
| AP22571PU-N | HPGD (255-266) antibody |
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 |
Goat | Aff - Purified | Hu, Mky | E, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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