HPV18 E7 Protein antibody
Principal name
HPV18 E7 Protein antibody
Alternative names for HPV18 E7 Protein antibody
Human Papilloma Virus Early protein 7
SwissProt ID
P06788 (Hpv18)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (capture) (E(capture)), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)
Background of HPV18 E7 Protein antibody
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
IF analysis of HPV-18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus-localized HPV-18 E7 detected by Mab 8E2. IF analysis of E7 protein show similar result also in HeLa cells.
Western blot analysis of HPV-18 E7 protein. WB was carried out with 8E2 Mab using the lysates of U20S, Hela and SiHa cells. Line 1. U20S with plasmid expressing E7 protein, Line 2. plain U2OS cells. Lines 3 and 4. Lysate of Hela and SiHa cells accordingly. The band in the 3rd lane represents positive control (Hela contains integrated HPV-18) Line 5. Protein sizemarker.
IP analysis of HPV-18 E7 protein. 1: detection of E7 protein by Mab 8E2 in lysate of U20S cells. 2: the lower band is precipitated E7, upper bands are light and heavy chain of Mab. 3: precipitation without mab 8E2. 4: IP exp. with plain U2OS cells. 5: IP exp. with plain Hela cells, where the lower band is non-specific binding. 6: marker.
IF analysis of HPV-18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV-18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleuslocalized HPV-18 E7 detected by Mab 10A8. IF analysis of E7 protein show similar result also in HeLa cells.
Western-Blot analysis of HPV-18 E7 protein. WB was carried out with 10A8 Mab using the lysates of U20S, Hela and SiHa cells. Line 1: U20S with plasmid expressing E7 protein, Line 2. Non-transfectedplain U2OS cells. Lines 3 and 4. Lysate of Hela and SiHa cells accordingly. The band in the 3rd lane represents positive control (Hela contains integrated HPV-18). Line 5. Protein size marker.
IP analysis of HPV-18 E7 protein. Line 1. detection of E7 protein by Mab 10A8 in lysate of U20S cells. Line 2. the lower band is precipitated E7, upper bands are light and heavy chain of Mab. Line 3. precipitation without mab 10A8. Line 4. IP exp. with non-transfected U2OS cells. Line 5. IP exp. Nontransfected Hela cells (Hela contains integrated HPV-18). Line 6. Protein size marker.