HRAS antibody
Principal name
HRAS antibody
Alternative names for HRAS antibody
GTPase HRas, HRAS1, p21ras, H-Ras-1, c-H-ras, Ha-Ras, H-Ras, v-Ha-ras Harvey rat sarcoma viral oncogene homolog
Gene ID
3265 (HRAS)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Luminex (LMNX)
Background of HRAS antibody
This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in sigl transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the pretal stage, growth deficiency at the posttal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene. [provided by RefSeq, Jul 2008].
General readings
Ma, Z., et al. Oncogene 29(41):5559-5567(2010)
van Engen-van Grunsven, A.C., et al. Am. J. Surg. Pathol. 34(10):1436-1441(2010)
Li, H., et al. Oncogene 29(36):5083-5094(2010)
Kwack, K.B., et al. Korean J Gastroenterol 56(2):78-82(2010)
Amosenko, F.A., et al. Genetika 46(5):700-708(2010)
Western Blot analysis of HRAS expression in transfected 293T cell line (H00003265-T02) by HRAS MaxPab polyclonal antibody.Lane 1: HRAS transfected lysate(20.79 KDa).Lane 2: Non-transfected lysate.
Western Blotting analysis (reducing conditions) of H-Ras in whole cell lysate using anti-H-Ras (H-RAS-03). Lane 1: HeLa human cervix carcinoma cell line. Lane 2: K567 human leukemia cell line. Lane 3: RAJI human Burkitt lymphoma cell line.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with HRAS antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-HRAS Antibody (Center) in Jurkat cell line lysates (35ug/lane). HRAS (arrow) was detected using the purified Pab.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PIK3R1 and HRAS HeLa cells were stained with anti-PIK3R1 rabbit purified polyclonal 1:1200 and anti-HRAS mouse purified polyclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Western Blot analysis of HRAS expression in transfected 293T cell line (H00003265-T02) by HRAS MaxPab polyclonal antibody.Lane 1: HRAS transfected lysate(20.79 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HRAS expression in transfected 293T cell line (H00003265-T01) by HRAS MaxPab polyclonal antibody.Lane 1: HRAS transfected lysate(21.30 KDa).Lane 2: Non-transfected lysate.
Western Blotting analysis ( reducing conditions ) of HRAS in whole cell lysate using HRAS monoclonal antibody, clone H-RAS-03 ( Cat # MAB3617 ).Lane 1 : HeLa, human cervix carcinoma cell line.Lane 2 : K-567, human leukemia cell line.Lane 3 : RAJI, human Burkitt lymphoma cell line.
Recombinant human HRAS, 1-186 aa, His-tagged: 15% SDS-PAGE (3 µg)
Confocal immunofluorescent analysis of HRAS Antibody (C-term) Cat.-No AP52096PU-N with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of HRAS Antibody (C-term) Cat.-No AP52096PU-N in mouse cerebellum tissue lysates (35ug/lane). This demonstrates the HRAS antibody detected the HRAS protein (arrow).
Western blot analysis of HRAS (arrow) using HRAS Antibody (C-term) Cat.-No AP52096PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HRAS gene.
Anti-HRAS mouse monoclonal antibody (TA505665) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HRAS monoclonal antibody at 1:200 (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505666)
Anti-HRAS mouse monoclonal antibody (TA505666) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-HRAS monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505667)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505667)
Anti-HRAS mouse monoclonal antibody (TA505667) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HRAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-HRAS mouse monoclonal antibody (TA505668) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505669)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505669)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505669)
Immunofluorescent staining of HeLa cells using anti-HRAS mouse monoclonal antibody (TA505669).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HRAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HRAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505675)
Anti-HRAS mouse monoclonal antibody (TA505675) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-HRAS monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HRAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HRAS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505676)
Anti-HRAS mouse monoclonal antibody (TA505676) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HRAS(RC216409).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HRAS (RC216409, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HRAS.
Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-HRAS monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HRAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HRAS Luminex Elisa with 1G8 Capture (TA600224) and 1A1 Detection (TA700224) Antibodies. Substrate used: Recombinant Human HRAS (TP316409)
HRAS Luminex Elisa with 1G8 Capture (TA600224) and 1A1 Detection (TA700224) Antibodies. Substrate used: Recombinant Human HRAS (TP316409)
H-Ras antibody detects HRAS protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. H-Ras antibody (TA308498) dilution: 1:1000
Sample (30 ug of whole cell lysate). A: Hela. B: Hep G2 . C: Molt-4 . 12% SDS PAGE. TA308498 diluted at 1:1000.
Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using H-Ras (TA308498) antibody at 1:500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras (TA308498) antibody at 1:200 dilution.
Western blot analysis of extracts from HeLa cells, using RASH/RASK/RASN antibody (#TA326254).The lane on the right is treated with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using RASH/RASK/RASN antibody (#TA326254).The picture on the right is treated with the synthesized peptide.
Gel: 10%SDS-PAGE, Lysate: 30 ug, Lane 1-4: Hela cells, HepG2 cells, 231 cells, Mouse kidney tissue, Primary antibody: (HRAS Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
- Quick-Filters:
- Highly Validated (6)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308498 | HRAS antibody |
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| Rabbit polyclonal antibody to H-Ras (v-Ha-ras Harvey rat sarcoma viral oncogene homolog) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Rt | ICC/IF, P, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody! |
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| TA505666 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTIC8 (formerly C8) | |||||||
 |
Mouse | IgG1 | OTIC8 | Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA505667 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTIC2 (formerly C2) | |||||||
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Mouse | IgG2b | OTIC2 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA505669 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTID9 (formerly D9) | |||||||
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Mouse | IgG1 | OTID9 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA505675 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTIA1 (formerly A1) | |||||||
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Mouse | IgG1 | OTIA1 | Purified | Can, Hu, Ms | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA505676 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTI1A1 (formerly 1A1) | |||||||
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Mouse | IgG1 | OTI1A1 | Purified | Can, Hu, Mky, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
|
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| TA326254 | HRAS antibody |
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| Rabbit polyclonal RASH/RASK/RASN antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA350625 | HRAS antibody |
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| Rabbit Polyclonal Anti-HRAS Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA505665 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTI1F2 (formerly 1F2) | |||||||
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Mouse | IgG1 | OTI1F2 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA505668 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTIG8 (formerly G8) | |||||||
 |
Mouse | IgG1 | OTIG8 | Purified | Hu | ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA505674 | HRAS antibody |
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| HRAS mouse monoclonal antibody, clone OTI1H3 (formerly 1H3) | |||||||
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Mouse | IgG1 | OTI1H3 | Purified | Can, Hu, Mky, Ms, Rt | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA600224 | HRAS antibody |
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| Luminex validated HRAS mouse monoclonal antibody (clone 1G8, clone OTI1G8 (formerly 1G8) | |||||||
 |
Mouse | IgG1 | OTI1G8 | Purified | Hu | LMNX |
0.1 mg /
€350.00
|
| OriGene Technologies, Inc. | |||||||
| TA700224 | HRAS antibody |
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| Luminex validated biotinylated HRAS mouse monoclonal antibody (clone 1A1), clone OTI1A1 (formerly 1A1) | |||||||
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Mouse | IgG1 | OTI1A1 | Purified | Hu | LMNX |
50 µg /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| AM03035PU-N | HRAS antibody |
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 |
Mouse | IgG1 | H-Ras-03 | Aff - Purified | Hu | E, WB |
0.1 mg /
€230.00
|
| OriGene Technologies GmbH | |||||||
| AP14550PU-N | HRAS (Center) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP52096PU-N | HRAS (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu, Ms | ICC/IF, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP316409 | HRAS (transcript variant 1) |
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| Recombinant protein of human v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP325202 | HRAS (transcript variant 3) |
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| Recombinant protein of human v-Ha-ras Harvey rat sarcoma viral oncogene homolog (HRAS), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY406123 | HRAS overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY430451 | HRAS overexpression lysate |
|
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0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |