HSP60 antibody
Principal name
HSP60 antibody
Alternative names for HSP60 antibody
HSP-60, HSPD1, Heat shock protein 60, Chaperonin 60, HuCHA60, GROEL, GroEL Homolog, CPN60, 60 kDa heat shock protein mitochondrial, 60 kDa chaperonin, Mitochondrial matrix protein P1, P60 lymphocyte protein
SwissProt ID
P0A6F5 (Ecoli), P10809 (Human), P31081 (Bovin), P63038 (Mouse), P63039 (Rat), Q3KMQ9 (Chlta)
Ncbi ID
AP_004644.1, 9606, NP_002147, NP_002147.2, 83333, NP_955472, NP_071565.2, AAH02676.1, NP_418567, NP_034607.3, NM_022229, NM_002156
Available reactivities
Available hosts
Available applications
Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Radioimmunoassay (R), EPair for Elisa (EPair), Immunoprecipitation (IP)
Background of HSP60 antibody
Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divegent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species.The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, artherosclerosis and chlamydial disease.
General readings
Cheng MY., et al. (1990), Nature. 348: 455- 458Ghosh JC., et al. (2008), J Biol Chem. Feb 22; 283(8):5188-94
Immunohistochemistry:
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60Cat.No. AM03128PU (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60Cat.No. AM03128PU (1/50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Flow cytometry analysis of HSP60 in A549 cell line, staining at 2-5ug for 1x106 cells. The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate.
ICC/IF analysis of HSP60 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human HSP60 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green)
Western blot analysis: Cell lysates of Hela (30 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HSP60 Cat.No. AM03128PU (1/1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analyis of Hsp60 in HeLa cell lysates using a 1:1000 dilution of the antibody
IHC analysis of Listeria infected mice spleens 6 days after infection with L-monocytogenes using the antibody
Hsp60 visualized using the antibody
Immunohistochemistry (IHC) analyzes of HSP60/HSPD1 antibody (Cat.-No.: AP06174PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of HSP60/HSPD1 antibody (Cat.-No.: AP06174PU-N) in extracts from COLO205 cells.
Western blot analysis of cell lysates of Hela ( 30 µg ) were resolved by SDS - PAGE , transferred to NC membrane and probed with HSPD1 monoclonal antibody , clone 2E4 ( 1 : 1000 ) ( Cat # MAB1121 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of purified MaxPab antibody to HSPD1 on HeLa cell. [antibody concentration 10 ug/ml]
HSPD1 MaxPab polyclonal antibody. Western Blot analysis of HSPD1 expression in human kidney.
Western Blot analysis of HSPD1 expression in transfected 293T cell line (H00003329-T01) by HSPD1 MaxPab polyclonal antibody.Lane 1: HSPD1 transfected lysate(63.03 KDa).Lane 2: Non-transfected lysate.
HSPD1 MaxPab polyclonal antibody. Western Blot analysis of HSPD1 expression in HepG2.
Human colon ca. stained with Anti-HSP60, cat# AP15594PU
Western Blot analysis of HeLa cell lysate with HSP60 antibody
Adrenal: Formalin-Fixed Paraffin-Embedded (FFPE)
Liver: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunofluorescence of purified MaxPab antibody to HSPD1 on HeLa cell. [antibody concentration 10 ug/ml]
HSPD1 MaxPab polyclonal antibody. Western Blot analysis of HSPD1 expression in human kidney.
Western Blot analysis of HSPD1 expression in transfected 293T cell line (H00003329-T02) by HSPD1 MaxPab polyclonal antibody.Lane 1: HSPD1 transfected lysate(63.03 KDa).Lane 2: Non-transfected lysate.
HSPD1 MaxPab polyclonal antibody. Western Blot analysis of HSPD1 expression in HepG2.
HSPD1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPD1 expression in human liver.
Western Blot analysis of HSPD1 expression in transfected 293T cell line (H00003329-T01) by HSPD1 MaxPab polyclonal antibody.Lane 1: HSPD1 transfected lysate(61.10 KDa).Lane 2: Non-transfected lysate.
HSPD1 MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPD1 expression in A-431.
Immunohistochemical staining of HSPD1 on formalin fixed, paraffin embedded human colon cancer with HSPD1 polyclonal antibody.
Western blot analysis of Hsp60 in cell lysates from 12 rat tissue lines using a 1:1000 dilution of the antibody
IHC of human skin fibroblasts (Left: control, Right: 24 hours after 7th passage of senescence).
Human fibroblast mitochondria; . Lanes: Lane1: 40ug human fibroblast mitochondria. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-Rabbit HRP. Secondary Antibody Dilution: 1:5000. Gene Name: HSPD1. Submitted by: Oleh Khalimonchuk, University of Nebraska-Lincoln.; HSPD1 antibody - C-terminal region (AVARP09014_P050) in Human fibroblast mitochondria cells using Western Blot
Human Liver; WB Suggested Anti-HSPD1 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; HSPD1 antibody - C-terminal region (AVARP09014_P050) in Human Liver cells using Western Blot
Human Uterus (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody AP22882PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Spleen (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody AP22882PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Adrenal (formalin-fixed, paraffin-embedded) stained with HSPD1 antibody AP22882PU-N followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
HSP60 Polyclonal Antibody
Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 antibody Cat.-No AM06606SU-N with DAB staining.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 antibody Cat.-No AM06606SU-N with DAB staining.
Immunofluorescence analysis of 3T3-L1 cells using HSP60 antibody Cat.-No AM06606SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using HSP60 antibody Cat.-No AM06606SU-N with DAB staining. against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Flow Cytometric analysis of Hela cells using HSP60 antibody Cat.-No AM06606SU-N (green) and negative control (purple).
Formalin-Paraffin Human Pancreas stained with HSP60 Antibody Cat.-No AM50115PU (Clone SPM253).
Immunohistochemical analysis of HSP60 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of HSP60 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of HSP60 expression in HeLa (A); NIH3T3 (B); KNRK (C); L929 (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800756)
Western blot analysis of extracts (10ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human). (1:2000)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800757)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800757)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800758)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800771)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800772)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800774)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HSPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA800847)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPD1 (RC201281, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPD1.
Western blot analysis of extracts from COLO205 cells, using HSP60 antibody (#TA311958).The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2), using HSP60 Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using HSP60 antibody (#TA311958).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using HSP60 antibody (#TA311958).The picture on the right is treated with the synthesized peptide.
Predicted cell location: Cytoplasm . Positive control: Human ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using HSPD1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm . Positive control: Human tonsil and renal cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil and renal cancer tissue using HSPD1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm . Positive control: Human tonsil and renal cancer tissue. Recommended dilution: 1/50-150 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil and renal cancer tissue using HSPD1 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using HSPD1 antibody at dilution 1/15, on the right is treated with the fusion protein. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HSPD1 antibody at dilution 1/12, on the right is treated with the fusion protein. (Original magnification: x200)
HSPD1 Antibody (C-term) (Cat. #TA324747) western blot analysis in A431,mouse NIH/3T3 cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HSPD1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HSPD1 Antibody (C-term) (Cat. #TA324747) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HSPD1 Antibody (Center) (Cat. #TA324748) western blot analysis in A431 cell line lysates (35ug/lane).This demonstrates the HSPD1 antibody detected the HSPD1 protein (arrow).
IF image of U251 cells stained with HSPD1 (Center) antibody. U251 cells were incubated with AP2859C HSPD1 (Center) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin . Pictures were taken on a Biorevo microscope (BZ-900, Keyence). Note the highly specific localization of the HSPD1 mainly to the mitochondria.
IF image of Hela cell stained with HSPD1 Antibody (Center)(Cat#TA324748). Hela cells were incubated with HSPD1 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HSPD1 immunoreactivity is localized to Mitochondria significantly.
Western blot analysis of Hsp 60 Antibody (N-term) (Cat. #TA324868) in mouse lung tissue lysates (35ug/lane). Hsp (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Hsp60 using a 1:1000 dilution of the antibody
Western blot analysis of extracts of various cell lines, using HSPD1 antibody.
Immunohistochemistry of paraffin-embedded human stomach cancer using HSPD1 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using HSPD1 antibody. Blue: DAPI for nuclear staining.
Western Blot: Hsp60 Antibody [TA336825] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7
Immunohistochemistry: Hsp60 Antibody [TA336825] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: Hsp60 Antibody [TA336825] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunocytochemistry/Immunofluorescence: Hsp60 Antibody [TA336825] - Hsp60 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: Hsp60 Antibody [TA336826] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7
Western Blot: Hsp60 Antibody [TA336826] - Western blot analysis of extracts from HeLa and COS cells using TA336826 Hsp60 antibody at 1:1000
Immunohistochemistry: Hsp60 Antibody [TA336826] - IHC staining of HSP60 in human kidney carcinoma using DAB with hematoxylin counterstain.
Immunocytochemistry/Immunofluorescence: Hsp60 Antibody [TA336826] - Confocal immunofluorescence analysis of HeLa cells using Hsp60 antibody at 1:50 (green). Nuclei were counterstained using DAPI (blue).
Immunocytochemistry/Immunofluorescence: Hsp60 Antibody [TA336826] - Confocal immunofluorescent analysis of HeLa cells using Hsp60 antibody (TA336826, 1:100). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin
Immunocytochemistry/Immunofluorescence: Hsp60 Antibody [TA336826] - HSP60 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Western blot detection of Hsp60 in Hela,Raji,Jurkat,C6,C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.
Immunocytochemistry staining of HeLa cells fixed with -20? Methanol and using Hsp60 mouse mAb (dilution 1:100).
Immunoprecipitation analysis of Hela cell lysates using Hsp60 mouse mAb.
Western blot analysis on COLO205 cell lysate using HSP60 Antibody
Western blot analysis of HSP60 expression in Hela (A); NIH3T3 (B); mouse brain (C); rat brain (D) whole cell lysates.
Immunohistochemical analysis of HSP60 staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western Blot analysis of HeLa cell lysate with HSP60 antibody
Human Colon Carcinoma stained with anti-Heat Shock Protein 60 (HSP60) antibody.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326374 | HSP60 antibody |
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| Mouse monoclonal Hsp60 Antibody | |||||||
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Mouse | IgG1 | LK2 | Purified | Bac, Bov, Can, Chk, GP, Hst, Hu, Mky, Ms, Pl, Por, Rb, Rt, whitefly, Ye | C, F, WB |
0.2 mg /
€385.00
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
Published recently! |
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| TA311958 | HSP60 antibody |
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| Rabbit polyclonal anti-HSP60 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA336825 | HSP60 antibody |
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| Rabbit Polyclonal HSP60 Antibody | |||||||
![Western Blot: Hsp60 Antibody [TA336825] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-77396-1-W.jpg) |
Rabbit | Purified | Hst, Hu, Ms, Prim, Rt | C, ICC/IF, P, WB |
0.1 ml /
€395.00
|
||
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody! |
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| TA336826 | HSP60 antibody |
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| Rabbit Polyclonal HSP60 Antibody | |||||||
![Western Blot: Hsp60 Antibody [TA336826] - Analysis of HSP60 in: 1. HeLa, 2. HepG2, 3. NIH/3T3, 4. Jurkat, 5. CHO, 6. A431, 7. PC12 and 8. COS7](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NBP1-77397-1-W.jpg) |
Rabbit | Purified | Hst, Hu, Ms, Prim, Rt | C, ICC/IF, P, WB |
0.1 ml /
€450.00
|
||
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody! |
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| TA800756 | HSP60 antibody |
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| Mouse monoclonal anti-HSPD1(HSP60) antibody, clone 4E4, Loadin, clone OTIE4 (formerly E4) | |||||||
 |
Mouse | IgG1 | OTIE4 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€295.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA800758 | HSP60 antibody |
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| HSPD1 mouse monoclonal antibody, clone OTIA2 (formerly A2) | |||||||
 |
Mouse | IgG2a | OTIA2 | Purified | Can, Hu, Mky, Ms | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA800771 | HSP60 antibody |
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| HSPD1 mouse monoclonal antibody, clone OTIG1 (formerly G1) | |||||||
 |
Mouse | IgG2a | OTIG1 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA800772 | HSP60 antibody |
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| HSPD1 mouse monoclonal antibody, clone OTIF7 (formerly F7) | |||||||
 |
Mouse | IgG1 | OTIF7 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA800774 | HSP60 antibody |
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| HSPD1 mouse monoclonal antibody, clone OTIF11 (formerly F11) | |||||||
 |
Mouse | IgG1 | OTIF11 | Purified | Can, Hu, Mky, Ms, Rt | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +14 additional images |
Highly validated antibody!
|
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| TA800847 | HSP60 antibody |
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| HSPD1 (Hsp60) mouse monoclonal antibody, clone OTI3G10 (formerly 3G10) | |||||||
 |
Mouse | IgG1 | OTI3G10 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +12 additional images |
Highly validated antibody!
|
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| AM03128PU-N | HSP60 (1-573) antibody |
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 |
Mouse | IgG1 | 2E4 | Purified | Hu | E, F, ICC/IF, P, WB |
0.1 ml /
€330.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM03128PU-S | HSP60 (1-573) antibody |
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|
Mouse | IgG1 | 2E4 | Purified | Hu | E, F, ICC/IF, P, WB |
50 µl /
€220.00
|
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM06606SU-N | HSP60 antibody |
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 |
Mouse | IgG1 | 3G8 | Ascites | Hu, Mky, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€410.00
|
| OriGene Technologies GmbH | |||||||
| +5 additional images |
Highly validated antibody! |
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| AP22882PU-N | HSP60 antibody |
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 |
Goat | Purified | Bac, Bov, Can, Dros, Fi, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen | IP, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
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| AM33359PU-S | HSP60 antibody |
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 |
Mouse | IgG1 | GROEL730 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM33359PU-T | HSP60 antibody |
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|
Mouse | IgG1 | GROEL730 | Purified | Hu | C, F, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
OriGene recommends this product for Immunohistochemistry. |
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| AM50115PU-S | HSP60 antibody |
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 |
Mouse | IgG1 | SPM253 | Purified | Bov, Can, Chk, Dros, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen | C, ICC/IF, IP, P, WB |
0.1 mg /
€430.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
OriGene recommends this product for Immunohistochemistry. |
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| AM50115PU-T | HSP60 antibody |
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|
Mouse | IgG1 | SPM253 | Purified | Bov, Can, Chk, Dros, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen | C, ICC/IF, IP, P, WB |
20 µg /
€240.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
OriGene recommends this product for Immunohistochemistry. |
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| TA321441 | HSP60 antibody |
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| Anti-HSPD1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321442 | HSP60 antibody |
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| Anti-HSPD1 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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