HSPA1L antibody
Principal name
HSPA1L antibody
Alternative names for HSPA1L antibody
HSP70-Hom, HSP70-1-like, Heat shock 70 kDa protein 1L, Heat shock 70 kDa protein 1-Hom
Ncbi ID
9606, NP_005518, NP_005518.3, AAH34483, NM_005527, NP_038586.2, NM_212546
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of HSPA1L antibody
- Kit Component:
- - KN308002G1, Hspa1l gRNA vector 1 in pCas-Guide vector
- KN308002G2, Hspa1l gRNA vector 2 in pCas-Guide vector
- KN308002D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Buraczynska,M., (er) Clin. Sci. (2008) In press
Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-HSPA1L mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
HSPA1L monoclonal antibody (M02), clone 7H6 Western Blot analysis of HSPA1L expression in HeLa ( Cat # L013V1 ).
HSPA1L monoclonal antibody (M02), clone 7H6. Western Blot analysis of HSPA1L expression in PC-12 ( Cat # L012V1 ).
Detection limit for recombinant GST tagged HSPA1L is approximately 0.1ng/ml as a capture antibody.
HSPA1L polyclonal antibody (A01), Lot # 060509JCS1 Western Blot analysis of HSPA1L expression in K-562 ( Cat # L009V1 ).
HSPA1L monoclonal antibody (M01), clone 1B5. Western Blot analysis of HSPA1L expression in PC-12 ( Cat # L012V1 ).
HSPA1L monoclonal antibody (M01), clone 1B5. Western Blot analysis of HSPA1L expression in HeLa ( Cat # L013V1 ).
Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPA1L and TP53 HeLa cells were stained with anti-HSPA1L rabbit purified polyclonal 1:1200 and anti-TP53 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
HSPA1L MaxPab rabbit polyclonal antibody. Western Blot analysis of HSPA1L expression in mouse testis.
Western Blot analysis of HSPA1L expression in transfected 293T cell line (H00003305-T01) by HSPA1L MaxPab polyclonal antibody.Lane 1: HSPA1L transfected lysate(70.40 KDa).Lane 2: Non-transfected lysate.
Rat, Lamprey; . Sample Type: 1. Lamprey CNS (20ug). 2. Rat Brain Lysate (20ug) . Primary Dilution: 1:1000. Secondary Antibody: Goat anti-Rabbit HRP. Secondary Dilution: 1:4000. Image Submitted by: Jennifer Morgan, PhD & David Busch. University of Texas in Austin .; HSPA1L antibody - C-terminal region (ARP53636_P050) in Rat, Lamprey cells using Western Blot
Human Jurkat; WB Suggested Anti-HSPA1L Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; HSPA1L antibody - C-terminal region (ARP53636_P050) in Human Jurkat cells using Western Blot
Western blot analysis of extracts of various cell lines, using HSPA1L antibody.
Immunofluorescence analysis of U2OS cell using HSPA1L antibody. Blue: DAPI for nuclear staining.
WB Suggested Anti-HSPA1L Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
Sample Type: 1. Lamprey CNS (20ug); 2. Rat Brain Lysate (20ug); Primary Dilution: 1: 1000; Secondary Antibody: Goat anti-Rabbit HRP; Secondary Dilution: 1: 4000; Image Submitted by: Jennifer Morgan, PhD & David Busch; University of Texas in Austin
Western blot analysis of HSP70 expression in Hela (A); mouse brain (B) whole cell lysates.
Immunohistochemical analysis of HSP70 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of HSP70 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA327201 | HSPA1L antibody |
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| HSPA1L Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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Lysates
| Catalog No. | ||
|---|---|---|
| LY417244 | HSPA1L overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR206495L2V | Lenti ORF particles, Hspa1l (GFP-tagged ORF) - Rat heat shock 70kD protein 1-like (mapped) (Hspa1l), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,380.00
|
|
| OriGene Technologies, Inc. | ||
| RR206495L1V | Lenti ORF particles, Hspa1l (Myc-DDK-tagged ORF) - Rat heat shock 70kD protein 1-like (mapped) (Hspa1l), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
