HSPBP1 antibody
Principal name
HSPBP1 antibody
Alternative names for HSPBP1 antibody
Heat shock protein-binding protein 1, Hsp70-binding protein 1, HSPBP, HspBP2, Hsp70-interacting protein 1, Hsp70-binding protein 2, Hsp70-interacting protein 2
Ncbi ID
9606, NP_001123578, AAH01236, NM_139261, NP_001123578.1, NM_001130106, NP_640354.1, NP_077134.1, NM_024172, NM_012267
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of HSPBP1 antibody
- Kit Component:
- - KN308018G1, 1500019G21Rik gRNA vector 1 in pCas-Guide vector
- KN308018G2, 1500019G21Rik gRNA vector 2 in pCas-Guide vector
- KN308018D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Graner, M.W., et al. Cancer Sci. 100(10):1870-1879(2009)
Evdonin, A., et al. Biol. Cell 101(6):351-360(2009)
Souza, A.P., et al. Cell Stress Chaperones 14(3):301-310(2009)
Howarth, J.L., et al. J. Neurochem. 108(4):945-951(2009)
Snyers, L., et al. Biochem. Biophys. Res. Commun. 368(3):767-771(2008)
Western blot of HSPBP1 polyclonal antibody ( Cat # PAB0882 ) in 15 ug/protein from MCF-7 cells per lane and probed with 1 : 1000 dilution of th affinity purified antibody.
Western Blot analysis of HSPBP1 expression in transfected 293T cell line (H00023640-T01) by HSPBP1 MaxPab polyclonal antibody.Lane 1: HSPBP1 transfected lysate(39.30 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of HSPBP1 transfected lysate using anti-HSPBP1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HSPBP1 MaxPab mouse polyclonal antibody (B01) (H00023640-B01).
Recombinant human HSPBP1, 1- 362 aa, His-tagged: 15% SDS-PAGE (3 ug)
Rat Muscle; WB Suggested Anti-Hspbp1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat Muscle; Hspbp1 antibody - C-terminal region (ARP54869_P050) in Rat Muscle cells using Western Blot
Anti-HSPBP1 mouse monoclonal antibody (TA503311) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPBP1(RC201814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPBP1 (RC201814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPBP1.
Western blot analysis of extracts (10ug) from 1 cell line by using anti-HSPBP1 monoclonal antibody.(1:200)
HEK293T cells transfected with either RC201814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPBP1 antibody(TA503311), and then analyzed by flow cytometry.
Anti-HSPBP1 mouse monoclonal antibody (TA503317) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPBP1(RC201814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPBP1 (RC201814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPBP1.
HEK293T cells transfected with either RC201814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPBP1 antibody(TA503317), and then analyzed by flow cytometry.
Anti-HSPBP1 mouse monoclonal antibody (TA503318) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPBP1(RC201814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPBP1 (RC201814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPBP1.
HEK293T cells transfected with either RC201814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPBP1 antibody(TA503318), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503412)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503412)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503412)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503412)
Anti-HSPBP1 mouse monoclonal antibody (TA503412) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPBP1(RC201814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPBP1 (RC201814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPBP1.
HEK293T cells transfected with either RC201814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPBP1 antibody(TA503412), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HSPBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503467)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPBP1 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPBP1 (RC201814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPBP1.
HEK293T cells transfected with either RC201814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPBP1 antibody(TA503467), and then analyzed by flow cytometry.
Anti-HSPBP1 mouse monoclonal antibody (TA503554) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPBP1(RC201814).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPBP1 (RC201814, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPBP1.
HEK293T cells transfected with either RC201814 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HSPBP1 antibody(TA503554), and then analyzed by flow cytometry.
HSPBP1 Antibody (N-term) (Cat. #TA324565) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the HSPBP1 antibody detected the HSPBP1 protein (arrow).
HSPBP1 Antibody (N-term) (TA324565)immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HSPBP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Host: Rabbit; Target Name: Hspbp1; Sample Tissue: Rat Spleen lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (3)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503311 | HSPBP1 antibody |
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| HSPBP1 mouse monoclonal antibody, clone OTI2C8 (formerly 2C8) | |||||||
 |
Mouse | IgG1 | OTI2C8 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
 +3 additional images |
Highly validated antibody!
|
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| TA503412 | HSPBP1 antibody |
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| HSPBP1 mouse monoclonal antibody, clone OTI1F3 (formerly 1F3) | |||||||
 |
Mouse | IgG2b | OTI1F3 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA503467 | HSPBP1 antibody |
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| HSPBP1 mouse monoclonal antibody, clone OTI1D5 (formerly 1D5) | |||||||
 |
Mouse | IgG1 | OTI1D5 | Purified | Hu, Mky | F, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +9 additional images |
Highly validated antibody!
|
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| TA324565 | HSPBP1 (N-term) antibody |
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| Rabbit polyclonal HSPBP1 Antibody (N-term) | |||||||
 |
Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA503317 | HSPBP1 antibody |
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| HSPBP1 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4) | |||||||
 |
Mouse | IgG1 | OTI1H4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503318 | HSPBP1 antibody |
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| HSPBP1 mouse monoclonal antibody, clone OTI2D1 (formerly 2D1) | |||||||
 |
Mouse | IgG1 | OTI2D1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
||||||
| TA503554 | HSPBP1 antibody |
||||||
| HSPBP1 mouse monoclonal antibody, clone OTI3E1 (formerly 3E1) | |||||||
 |
Mouse | IgG1 | OTI3E1 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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Lysates
| Catalog No. | ||
|---|---|---|
| LY427167 | HSPBP1 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR205444L2V | Lenti ORF particles, Hspbp1 (GFP-tagged) - Mouse HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 (Hspbp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR205444L1V | Lenti ORF particles, Hspbp1 (Myc-DDK-tagged) - Mouse HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 (Hspbp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR205086L2V | Lenti ORF particles, Hspbp1 (GFP-tagged ORF) - Rat HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 (Hspbp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR205086L1V | Lenti ORF particles, Hspbp1 (Myc-DDK-tagged ORF) - Rat HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 (Hspbp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||