IFITM3 antibody
Principal name
IFITM3 antibody
Alternative names for IFITM3 antibody
1-8U, Interferon-induced transmembrane protein 3, Interferon-inducible protein 1-8U
SwissProt ID
Q01628 (Human)
Gene ID
10410 (IFITM3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)
Background of IFITM3 antibody
- Kit Component:
- - KN201635G1, IFITM3 gRNA vector 1 in pCas-Guide vector
- KN201635G2, IFITM3 gRNA vector 2 in pCas-Guide vector
- KN201635D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
IFITM3 monoclonal antibody (M01), clone 4C8-1B10 Western Blot analysis of IFITM3 expression in Hela ( Cat # L013V1 ).
Detection limit for recombinant GST tagged IFITM3 is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of IFITM3 expression in transfected 293T cell line (H00010410-T01) by IFITM3 MaxPab polyclonal antibody.Lane1:IFITM3 transfected lysate(14.74 KDa).Lane 2:Non-transfected lysate.
Western blot analysis of IFITM3 polyclonal antibody ( Cat # PAB2360 ) in 293 cell line lysates ( 35 µg/lane ) . IFITM3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
Western blot analysis of IFITM3 ( arrow ) using rabbit IFITM3 polyclonal antibody ( Cat # PAB2361 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the IFITM3 gene ( Lane 2 ) ( Origene Technologies ) .
Western blot analysis of IFITM3 polyclonal antibody ( Cat # PAB2361 ) in Hela cell line lysates ( 35 µg/lane ) . IFITM3 ( arrow ) was detected using the purified polyclonal antibody ( 1 : 60 dilution ) .
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in human placenta.
Western Blot analysis of IFITM3 expression in transfected 293T cell line (H00010410-T02) by IFITM3 MaxPab polyclonal antibody.Lane 1: IFITM3 transfected lysate(14.60 KDa).Lane 2: Non-transfected lysate.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in HepG2.
(Left) Western blot analysis of IFITM3 (arrow) using rabbit polyclonal IFITM3 Antibody (Center) (Cat.#AP11168PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the IFITM3 gene (Lane 2) (Origene Technologies). (Right) Western blot analysis of anti-IFITM3 Antibody (Center) (Cat# AP11168PU-N) in Hela cell line lysates (35 µg/lane). IFITM3(arrow) was detected using the purified Pab (1/60 dilution).
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in mouse lung.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in human liver.
Western Blot analysis of IFITM3 expression in transfected 293T cell line (H00010410-T01) by IFITM3 MaxPab polyclonal antibody.Lane 1: IFITM3 transfected lysate(14.74 KDa).Lane 2: Non-transfected lysate.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in HepG2.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in HepG2.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in human placenta.
Immunoprecipitation of IFITM3 transfected lysate using anti-IFITM3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IFITM3 MaxPab mouse polyclonal antibody (B02) (H00010410-B02).
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in mouse lung.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in human liver.
IFITM3 MaxPab rabbit polyclonal antibody. Western Blot analysis of IFITM3 expression in HepG2.
Immunoprecipitation of IFITM3 transfected lysate using anti-IFITM3 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IFITM3 MaxPab mouse polyclonal antibody (B01) (H00010410-B01).
Human IFITM3 (Interferon-induced transmembrane protein 3) recombinant protein
Human IFITM3 (Interferon-induced transmembrane protein 3) recombinant protein
Human IFITM3 (Interferon-induced transmembrane protein 3) recombinant protein
Immunofluorescent analysis of IFITM3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of IFITM3 expression in HEK293T (A); HeLa (B); Raw264.7 (C); H9C2 (D) whole cell lysates.
Western blot analysis of extracts from HepG2 cells and HeLa cells, using IFM3 antibody.The lane on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF-7 cells, using IFM3 antibody.The picture on the right is treated with the synthesized peptide.
IFITM3 Antibody (N-term) (Cat. #TA324408) western blot analysis in Hela,293,A549,NCI-H460,HepG2,Sk-Br-3,A375 cell line lysates (35ug/lane).This demonstrates the IFITM3 antibody detected the IFITM3 protein (arrow).
Western blot analysis of lysate from HT1080 cell line,using IFITM3 Antibody (N-term)(Cat. #TA324408).TA324408 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
IF image of HeLa cells stained with IFITM3 (N-term) antibody. HeLa cells were incubated with Fragilis (IFITM3) (N-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin . Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the IFITM3 immunoreactivity to the Golgi.
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- Overnight Delivery (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315049 | IFITM3 antibody |
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| Rabbit polyclonal anti-IFM3 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, ICC/IF, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA324408 | IFITM3 (N-term) antibody |
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| Rabbit polyclonal Interferon-inducible protein (IFITM3) Antibody (N-term) | |||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA349530 | IFITM3 antibody |
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| Rabbit Polyclonal Anti-IFITM3 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| AP11168PU-N | IFITM3 antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412107 | IFITM3 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |