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IGFBP3 antibody

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Principal name

IGFBP3 antibody

Alternative names for IGFBP3 antibody

IGFBP-3, IGF-BP3, IBP3, Insulin-like growth factor-binding protein 3, IGF-binding protein 3, IBP-3

SwissProt ID

P15473 (Rat), P16611 (Pig), P17936 (Human), P20959 (Bovin), P47878 (Mouse)

Gene ID

3486 (IGFBP3), 24484 (Igfbp3), 16009

Ncbi ID

NP_000589.2, 9606, 10090, 10116, NP_000589, NP_000589.1, NM_001013398, NM_000598, NM_012588

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of IGFBP3 antibody

Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33.
Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria.

General readings

Lin J, Manson JE, Lee IM, Cook NR, Buring JE, Zhang SM.
Intakes of calcium and vitamin D and breast cancer risk in women.
Arch Intern Med. 2007 May 28;167(10):1050-9.
PMID: 17533208

PAB7567 - IGFBP3
IGFBP3 polyclonal antibody ( Cat # PAB7567 ) ( 0.02 µg/ml ) staining of human breast cancer lysate ( 35 µg protein in RIPA buffer ) with ( B ) and without ( A ) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
AP14345PU-N - IGFBP3
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AP14345PU-N IGFBP3 Antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14345PU-N - IGFBP3
Western blot analysis of AP14345PU-N IGFBP3 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). IGFBP3(arrow) was detected using the purified Pab (1/60 dilution).
AP14347PU-N - IGFBP3
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IGFBP3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
AP14347PU-N - IGFBP3
Western blot analysis of IGFBP3(arrow) using rabbit polyclonal IGFBP3 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with IGFBP gene (Lane 2)
H00003486-D01P - IGFBP3
Western Blot analysis of IGFBP3 expression in transfected 293T cell line (H00003486-T01) by IGFBP3 MaxPab polyclonal antibody.Lane 1: IGFBP3 transfected lysate(31.70 KDa).Lane 2: Non-transfected lysate.
AP20503PU-N - IGFBP3
Western blot (WB) analysis of IGFBP3 antibody (Cat.-No.: AP20503PU-N) in extracts from HUVEC cells.
A1052-1 - IGFBP3
Immunohistochemistry analysis of paraffin-embedded human brain, using IGFBP-3 (Phospho-Ser183) Antibody. The picture on the right is treated with the synthesized peptide.
B1052-1 - IGFBP3
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using IGFBP-3 (Ab-183) Antibody. The picture on the right is treated with the synthesized peptide.
B1052-1 - IGFBP3
Western blot analysis of extracts from RAW246.7 cells, using IGFBP-3 (Ab-183) Antibody. The lane on the right is treated with the synthesized peptide.
AR51811PU-N - IGFBP3
AP55946PU-S - IGFBP3
Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody AP55946PU-N.The lane on the right is treated with the antigen-specific peptide.
CEK1195 - Human IGFBP3 ELISA Kit
Representative Standard Curve
CEK1481 - Mouse IGFBP3 ELISA Kit
Representative Standard Curve
TA807469 - IGFBP3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3.
TA807472 - IGFBP3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3.
TA807823 - IGFBP3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3.(1:2000)
TP723171 - IGFBP3
TA303275 - IGFBP3
TA303275 (0.02µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
TA323616 - IGFBP3
Predicted band size: 24-41 kDa . Positive control: HepG2, 3T3, Hela, 293, Jurkat, K562, Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3,4,5,6,7: HepG2, 3T3, Hela, 293, Jurkat, K562, Raji cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
TA325554 - IGFBP3
Western blot analysis of IGFBP-3 expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA328352 - IGFBP3
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
TA350695 - IGFBP3
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (IGFBP3 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute
TA350695 - IGFBP3
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (IGFBP3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA350695 - IGFBP3
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (IGFBP3 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: ×200)
TA325555 - IGFBP3
Western blot analysis of IGFBP-3 phosphorylation expression in whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IGFBP3 antibody
  Goat Polyclonal Antibody against IGFBP3  
TA303275 (0.02µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

IGFBP3 antibody
  Anti-IGFBP3 Rabbit Polyclonal Antibody  
Predicted band size: 24-41 kDa . Positive control: HepG2, 3T3, Hela, 293, Jurkat, K562, Raji cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3,4,5,6,7: HepG2, 3T3, Hela, 293, Jurkat, K562, Raji cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

IGFBP3 antibody
  Rabbit Polyclonal IGFBP-3 Antibody  
Western blot analysis of IGFBP-3 expression in HuvEc whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

IGFBP3 antibody
  Rabbit Polyclonal IGFBP-3 (Ser183) Antibody (Phospho-specific)  
Western blot analysis of IGFBP-3 phosphorylation expression in whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms P
0.1 ml / €335.00
  OriGene Technologies, Inc.

IGFBP3 antibody
  Anti-Human IGF-BP3 Rabbit Polyclonal Antibody  
This antibody stained formalin-fixed, paraffin-embedded sections of normal human placenta. The recommended concentrations are 2.5ug/ml-0.5 ug/ml for two hours at room temperature. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €275.00
  OriGene Technologies, Inc.

IGFBP3 antibody
  Rabbit Polyclonal Anti-IGFBP3 Antibody  
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (IGFBP3 Antibody) at dilution 1/300 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

IGFBP3 antibody
  IGFBP3 mouse monoclonal antibody, clone OTI3B9 (formerly 3B9)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3. Mouse IgG2b OTI3B9 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

IGFBP3 antibody
  IGFBP3 mouse monoclonal antibody, clone OTI8E11 (formerly 8E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3. Mouse IgG2b OTI8E11 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

IGFBP3 antibody
  IGFBP3 mouse monoclonal antibody, clone OTI16B1 (formerly 16B1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGFBP3 (RC216898, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGFBP3.(1:2000) Mouse IgG1 OTI16B1 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

IGFBP3 antibody
IGFBP3 Rabbit Biotin Hu E, WB
50 µg / €340.00
  OriGene Technologies GmbH

IGFBP3 antibody
IGFBP3 Rabbit Biotin Hu E, WB
25 µg / €250.00
  OriGene Technologies GmbH

IGFBP3 antibody
IGFBP3 Rabbit Aff - Purified Hu E, WB
0.1 mg / €340.00
  OriGene Technologies GmbH

IGFBP3 antibody
IGFBP3 Rabbit Aff - Purified Hu E, WB
50 µg / €250.00
  OriGene Technologies GmbH

IGFBP3 (N-term) antibody
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AP14345PU-N IGFBP3 Antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

IGFBP3 antibody
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IGFBP3 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

IGFBP3 antibody
Western blot (WB) analysis of IGFBP3 antibody (Cat.-No.: AP20503PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €370.00
  OriGene Technologies GmbH

IGFBP3 pSer183 antibody
Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody AP55946PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
0.1 mg / €300.00
  OriGene Technologies GmbH

IGFBP3 pSer183 antibody
Western blot analysis of extracts from Rat brain tissue using IGFBP-3 (Phospho-Ser183) antibody AP55946PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms WB
50 µg / €230.00
  OriGene Technologies GmbH

IGFBP3 antibody
IGFBP3 Rat IgG2 11B16 Purified Ms WB
0.1 mg / €460.00
  OriGene Technologies GmbH

CRISPR

Catalog No. Species Donor Vector  

IGFBP3 - human gene knockout kit via CRISPR
IGFBP3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

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