IGFBP7 antibody
Principal name
IGFBP7 antibody
Alternative names for IGFBP7 antibody
IGF-binding protein 7, IGFBP-7, IBP7, IBP-7, IGFBP-rP1, MAC25, PSF, TAF, Insulin-like growth factor-binding protein 7, Prostacyclin-stimulating factor, PGI2-stimulating factor, Tumor-derived adhesion factor
Ncbi ID
NP_001544.1, 9606, NP_001544, NM_001553, NM_001253835, NP_032074.3, NM_001159518
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)
Background of IGFBP7 antibody
- Kit Component:
- - KN308184G1, Igfbp7 gRNA vector 1 in pCas-Guide vector
- KN308184G2, Igfbp7 gRNA vector 2 in pCas-Guide vector
- KN308184D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Akaogi K., et al (1994). Biochem. Biophys. Res. Commun. 198:1046-1053
Oh Y., et al (1996). J. Biol. Chem. 271:30322-30325
This antibody stained HeLa and U-251 MG cells. The primary antibody was incubated at 2.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Sandwich ELISA using IGFBP7 Antibody Cat.-No AP01109PU
Human Muscle; WB Suggested Anti-IGFBP7 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Muscle; IGFBP7 antibody - C-terminal region (ARP48174_P050) in Human Muscle cells using Western Blot
Representative Standard Curve
(1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human colon and ovarian cancer tissue using IGFBP7 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted cell location: Cytoplasm. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded Human colon and ovarian cancer tissue using IGFBP7 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Predicted band size: 29 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/400 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using IGFBP7 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
To detect hIGF-BP7 by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP7.
To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IGF-BP7 (TA328355) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP7.
To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP7 (TA328354) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.
To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP7 (TA328354) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.
To detect hIGF-BP7 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP7 (TA328354) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP7.
WB Suggested Anti-IGFBP7 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Muscle
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311209 | IGFBP7 (145-159) antibody |
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| Goat Anti-IGFBP7 (aa145-159) Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA323150 | IGFBP7 antibody |
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| Anti-IGFBP7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
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| TA323151 | IGFBP7 antibody |
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| Anti-IGFBP7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA328354 | IGFBP7 antibody |
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| Biotinylated Anti-Human IGF-BP7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
50 µg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA328355 | IGFBP7 antibody |
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| Anti-Human IGF-BP7 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P, WB |
0.1 mg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| AP01109BT-N | IGFBP7 antibody |
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 |
Rabbit | Biotin | Hu | E, WB |
50 µg /
€340.00
|
||
| OriGene Technologies GmbH | |||||||
| AP01109BT-S | IGFBP7 antibody |
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|
Rabbit | Biotin | Hu | E, WB |
25 µg /
€250.00
|
||
| OriGene Technologies GmbH | |||||||
| AP01109PU-N | IGFBP7 antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | E, WB |
0.1 mg /
€340.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP01109PU-S | IGFBP7 antibody |
||||||
|
Rabbit | Aff - Purified | Hu | E, WB |
50 µg /
€250.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309844 | IGFBP7 |
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| Recombinant protein of human insulin-like growth factor binding protein 7 (IGFBP7) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP723175 | IGFBP7 |
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| Purified recombinant protein of Human insulin-like growth factor binding protein 7 (IGFBP7). | |||||
 |
Human | > 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
25 µg /
€230.00
|
|
| OriGene Technologies, Inc. | |||||
ELISA Kits
| Catalog No. | ||
|---|---|---|
| EA100636 | Human IGFBP7 ELISA Kit |
|
|
1 kit /
€455.00
|
|
| OriGene Technologies, Inc. |
Lysates
| Catalog No. | ||
|---|---|---|
| LY419862 | IGFBP7 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |