IKKA antibody
Principal name
IKKA antibody
Alternative names for IKKA antibody
CHUK, TCF16, I kappa-B kinase alpha, IkBKA, IKK-alpha, IKK-A, IkappaB kinase, I-kappa-B kinase 1, NFKBIKA, IKK1, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, Nuclear factor NF-kappa-B inhibitor kinase alpha
Gene ID
1147 (CHUK)
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F)
Background of IKKA antibody
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization sigl of NF-kB thereby preventing translocation of NF-kB to the nucleus. Exterl stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kise complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g. In vitro, IKK1/a and IKK2/b can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kise (NIK) and MAP kise kise kise-1 (MEKK1), respectively. Targeted disruption of IKK1/a gene in mice results in skin and limb abnormalities and death of newborns.
General readings
1. Huang CC et al. Intermolecular binding between TIFA-FHA and TIFA-pT mediates tumor necrosis factor alpha stimulation and NF-?B activation. Mol Cell Biol 32:2664-73 (2012).
2. Prager GW et al. Urokinase (uPA) mediates endothelial cell survival via induction of the X-linked inhibitor of apoptosis protein (XIAP). Blood : (2008). WB .
Western blot analysis of IKK alpha in Jurkat whole cell lysate with SP1268P.
Immunocytochemical staining of Jurkat cells wth Rabbit anti Human IKK alpha (SP1509P)
Figure 1. Western blot analysis of IKK alpha in HeLa whole cell lysate with SP1509PS.
Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCB and CHUK HeLa cells were stained with anti-PRKCB rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml]
CHUK monoclonal antibody (M03), clone 4B8 Western Blot analysis of CHUK expression in HeLa ( Cat # L013V1 ).
Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml]
CHUK monoclonal antibody (M04), clone 2G4 Western Blot analysis of CHUK expression in Jurkat ( Cat # L017V1 ).
Proximity Ligation Analysis (PLA) of protein-protein interactions between IKBKB and CHUK. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
Figure 1. Immunohistochemical analysis of paraffin- embedded human colon carcinoma tissue, using IKKα (phospho-Thr23) antibody AP02417PU.
Figure 2. Western blot analysis of extracts using IKKα (phospho-Thr23) antibody AP02417PU.
Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue, using IKKα antibody AP02652PU.
Figure 2. Western blot analysis of extracts from 293 cells (Lane 1) and MDA-MB-435 cells (Lane 2 and 3), untreated or treated with EGF, using IKKα antibody AP02652PU.
Immunohistochemistry (IHC) analyzes of IKK-α antibody (Cat.-No.: AP06179PU-N) in paraffin-embedded human colon carcinoma tissue.
Western blot analysis of IKK-α antibody at 1/500 dilution:
Lane 1: Hela whole cell lysate treated with TNF-alpha.
Lane 2: RAW264.7 whole cell lysate treated with TNF-alpha.
Lane 3: RAW264.7 whole cell lysate treated with LPS.
Lane 4: PC12 whole cell lysate treated with LPS.
Lane 1: Hela whole cell lysate treated with TNF-alpha.
Lane 2: RAW264.7 whole cell lysate treated with TNF-alpha.
Lane 3: RAW264.7 whole cell lysate treated with LPS.
Lane 4: PC12 whole cell lysate treated with LPS.
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of 30 µg of total cell lysate from Daudi cells with CHUK monoclonal antibody, clone 14A231 ( Cat # MAB0052 ) at 1 µg/ml dilution.
Immunohistochemistry (IHC) analysis of IKK-alpha pThr23 antibody in paraffin-embedded human colon carcinoma tissue.
Western blot (WB) analysis of IKK-alpha pThr23 antibody in extracts from MDA-MB-435 cells treated with EGF.
Immunohistochemical staining with CHUK polyclonal antibody ( Cat # PAB10246 ) was diluted 1 : 500 to detect CHUK in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Immunocytochemistry of CHUK in Jurkat cells with CHUK polyclonal antibody ( Cat # PAB13399 ) at 1µg/ml.
Western blot analysis of CHUK in HeLa whole cell lysate with CHUK polyclonal antibody ( Cat # PAB13399 ) at 1 : 1000 dilution.
Immunocytochemistry of CHUK in HeLa cells with CHUK polyclonal antibody ( Cat # PAB13401 ) at 10 µg/ml.
Western blot analysis of CHUK in HeLa whole cell lysate with CHUK polyclonal antibody ( Cat # PAB13401 ) at 1 : 500 dilution.
Immunocytochemistry of CHUK in Jurkat cells with CHUK polyclonal antibody ( Cat # PAB13402 ) at 10 µg/ml.
Western blot analysis of CHUK in Jurkat whole cell lysate with CHUK polyclonal antibody ( Cat # PAB13402 ) at 1 : 500 dilution.
Western blot analysis of CHUK in transfected RBL cells with CHUK polyclonal antibody ( Cat # PAB8780 ) .
Immunoblot analysis of cell lysates.Lane 1 : HeLa cell lysate. Lnae 2 : Jurkat T cell lysate.Lane 3 : 293T cell lysate.
Immunohistochemistry analyzes of IKKα/β antibody (AP20307PU-N) in paraffin-embedded human breast carcinoma tissue.
Western blot(WB) analysis of IKKα/β antibody (Cat.-No.: AP20307PU-N) at 1/500 dilution
Lane1:THP-1 whole cell lysate
Lane2:HEK293T whole cell lysate
Lane3:Mouse brain tissue lysate
Lane4:Rat heart tissue lysate
Lane5:NIH-3T3 whole cell lysate
Lane1:THP-1 whole cell lysate
Lane2:HEK293T whole cell lysate
Lane3:Mouse brain tissue lysate
Lane4:Rat heart tissue lysate
Lane5:NIH-3T3 whole cell lysate
Immunohistochemistry (IHC) analyzes of IKKα/β antibody (Cat.-No.: AP20346PU-N) in paraffin-embedded human skeletal muscle tissue.
Western blot analysis of IKKα/β antibody (Cat.-No.: AP20346PU-N) in extracts from Jurkat cells.
Immunohistochemistry (IHC) analyzes of p-HER2 (pTyr1248) pAb in paraffin-embedded human skeletal muscle tissue.
Immunohistochemistry (IHC) analyzes of p-IKK-a/b (pSer176) pAb in paraffin-embedded human breast carcinoma tissue.
Immunohistochemistry (IHC) analyzes of IKKa/b pAb in paraffin-embedded human skeletal muscle tissue.
Immunohistochemistry (IHC) analyzes of IKK-a/b pAb in paraffin-embedded human breast carcinomatissue.
Western blot analysis using CHUK mouse mAb against truncated Trx-CHUK recombinant protein (Lane 1).
Western blot analysis using CHUK antibody Cat.-No AM06512SU-N against Raji (1), Jurkat (2) and THP-1 (3) cell lysate.
Immunofluorescence analysis of NIH/3T3 cells using CHUK antibody Cat.-No AM06518SU-N (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Western blot analysis using CHUK AM06518SU-N against Raji (1), Jurkat (2), THP-1 (3) and K562 (4) cell lysate.
Flow cytometric analysis of A549 cells using CHUK AM06518SU-N (blue) and negative control (red).
Western blot analysis of extracts from HepG2 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser180/181) antibody AP55885PU-N.The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) antibody AP55886PU-N.The lane on the right is treated with the antigen-specific peptide.
Immunohistochemical analysis of IKK alpha (pT23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of IKK alpha (pT23) expression in HeLa TNFa-treated (A); Raw264.7 TNFa-treated (B); PC12 LPS-treated (C) whole cell lysates.
Immunohistochemical analysis of IKK alpha staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of IKK alpha expression in HeLa (A); Raw264.7 (B); PC12 (C) whole cell lysates.
Immunohistochemical analysis of IKK alpha/beta (pS180/181) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot analysis of IKK alpha/beta (pS180/181) expression in HEK293T LPS-treated (A); Raw264.7 TNFa-treated (B); PC12 LPS-treated (C) whole cell lysates.
Western blot analysis of IKK alpha/beta (pS176/177) expression in HeLa LPS-treated (A); mouse brain (B); rat brain (C) whole cell lysates.
Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.
Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1ug/mL.
Immunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 ug/mL.
Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:500 dilution.
Immunofluorescence of IKKa in HeLa cells with IKKa antibody at 2 ug/mL.
Immunocytochemistry of IKK alpha in HeLa cells with IKK alpha antibody at 10 ug/mL.
Western blot analysis of IKK alpha in Jurkat whole cell lysate with IKK alpha antibody (C3) at 1:500 dilution.
Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 10 ug/mL.
Anti-IKKa antibody was diluted 1:500 to detect IKKa in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Predicted band size: 85 kDa . Positive control: Hela, 293 and 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2,3: Hela, 293 and 3T3 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CHUK (Ab-23) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: x200)
Western blot analysis of IKK-alpha expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of IKK-alpha/beta expression in HepG2 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of IKK-alpha/beta expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
TA326719 (1µg/ml) staining of HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of extracts of various cell lines, using CHUK antibody.
Immunofluorescence analysis of A549 cell using CHUK antibody. Blue: DAPI for nuclear staining.
Western Blot: IKK alpha Antibody (14A231) [TA336452] - analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: IKK alpha Antibody (14A231) [TA336452] - Formalin-fixed, paraffin-embedded human kidney (IMH-343) stained with IKK alpha antibody at 5 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM
Immunocytochemistry/Immunofluorescence: IKK alpha Antibody (14A231) [TA336452] - IKK alpha antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). An antibody dilutio
Flow Cytometry: IKK alpha Antibody (14A231) [TA336452] - Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents the isotype control antibody, red represents the IKK alpha antibody. Intracellular flow kit was
Gel: 6%SDS-PAGE, Lysate: 50 ug, Lane: Hela cells, Primary antibody: (CHUK Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (CHUK Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (CHUK Antibody) at dilution 1/15, on the right is treated with synthetic peptide. (Original magnification: ×200)
Predicted band size: 85 kDa . Positive control: 293 cells treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Cytoplasm; Nucleus. Positive control: Human colon carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human colon carcinoma tissue using CHUK (Phospho-Thr23) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
Western blot analysis of extracts from 3T3 cells, treated with TNF (20ng/ml, 30mins), using IKK-a (Phospho-Ser176)/IKK-Ã?(Phospho-Ser177) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from A549 cells (Lane 2) and 3T3 cells (Lane 3), using IKK-a (Phospho-Ser176) /IKK-Ã?(Phospho-Ser177) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using IKK-a (Phospho-Ser176)/IKK-ß (Phospho-Ser177) antibody.The picture on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA305976 | IKKA antibody |
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| Rabbit Polyclonal IKK alpha Antibody | |||||||
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Rabbit | IgG | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA305994 | IKKA antibody |
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| Rabbit Polyclonal IKK alpha Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA305995 | IKKA antibody |
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| Rabbit Polyclonal IKK alpha Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA311872 | IKKA antibody |
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| Rabbit polyclonal IKK-alpha (Ser176)/IKK-beta (Phospho-Ser177) antibody(Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA319147 | IKKA (C-term) antibody |
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| Rabbit polyclonal IKK alpha antibody | |||||||
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Rabbit | Serum | Hu, Ms, Rt | E, IP, WB |
0.1 ml /
€515.00
|
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| OriGene Technologies, Inc. | |||||||
| TA321322 | IKKA antibody |
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| Anti-CHUK (Phospho-Thr23) Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA321323 | IKKA antibody |
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| Anti-CHUK Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA325378 | IKKA antibody |
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| Rabbit Polyclonal IKK-alpha Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325379 | IKKA antibody |
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| Rabbit Polyclonal IKK-alpha/beta Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325380 | IKKA antibody |
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| Rabbit Polyclonal IKK-alpha/beta Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA326719 | IKKA antibody |
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| Goat Anti-CHUK Antibody | |||||||
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Goat | Aff - Purified | Hu | E, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
| TA327250 | IKKA antibody |
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| Rabbit anti-CHUK Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA336452 | IKKA antibody |
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| Mouse Monoclonal IKK alpha Antibody (14A231) | |||||||
![Western Blot: IKK alpha Antibody (14A231) [TA336452] - analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/NB100-56704-1-W.jpg) |
Mouse | IgG1 | 14A231 | Purified | Hu, Ms, Prim | F, ICC/IF, IP, P, WB |
0.1 mg /
€475.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA350751 | IKKA antibody |
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| Rabbit Polyclonal Anti-CHUK Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AM06253SU-N | IKKA antibody |
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Mouse | IgG1 | 7C10B6 | Ascites | Hu | E, WB |
0.1 ml /
€340.00
|
| OriGene Technologies GmbH | |||||||
| AM06512SU-N | IKKA antibody |
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Mouse | IgG1 | 3G12 | Ascites | Hu | E, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| AM06518SU-N | IKKA antibody |
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 |
Mouse | IgG1 | 3G12H9 | Ascites | Hu | E, F, ICC/IF, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP01612PU-N | IKKA pThr23 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€370.00
|
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| OriGene Technologies GmbH | |||||||
| AP02417PU-N | IKKA pThr23 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | P, WB |
0.1 mg /
€300.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP02417PU-S | IKKA pThr23 antibody |
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Rabbit | Aff - Purified | P, WB |
50 µg /
€230.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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