IL1RN / IL1RA antibody
Principal name
IL1RN / IL1RA antibody
Alternative names for IL1RN / IL1RA antibody
IL1F3, IL-1ra, IL-1RN, IRAP, IL1 inhibitor, ICIL-1RA, Interleukin-1 receptor antagonist protein
SwissProt ID
O18999 (Horse), O77482 (Bovin), P18510 (Human), P25085 (Mouse), P25086 (Rat), P26890 (Rabit), Q29056 (Pig), Q9BEH0 (Canfa)
Ncbi ID
9606, NP_000568, NP_000568.1, NP_001034790, NP_776214.1, AAH09745, NP_001034790.1, NP_071530, NM_173842, NM_000577, NP_776214, NP_776215, NM_173841, NM_001039701, NM_001159562, NM_173843, NM_022194
Available reactivities
Available hosts
Available applications
Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Frozen Sections (C), Functional assay (FN), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of IL1RN / IL1RA antibody
The interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring molecule that shares homology with IL-1α and IL-1β. It binds competitively to IL-1 receptors Type I and Type II with the same affinity as does IL-1, but does not induce any intracellular response. Two structural variants of IL-1Ra have been described: a 17 kDa form that is secreted from monocytes, macrophages, neutrophils, and other cells (sIL-1Ra) and an 18-kDa form that remains in the cytoplasm of keratinocytes and other epithelial cells, monocytes, and fibroblasts (icIL-1Ra). An additional 16-kDa intracellular isoform of IL-1Ra has recently been described in neutrophils, monocytes, and hepatic cells. Both of the major isoforms of IL-1Ra are transcribed from the same gene through the use of alternative first exons. The production of IL-1Ra is stimulated by many substances including adherent IgG, other cytokines, and bacterial or viral components. The tissue distribution of IL-1Ra in mice indicates that sIL-1Ra is found predominantely in peripheral blood cells, lungs, spleen, and liver, while icIL-1Ra is found in large amounts in skin. Studies in transgenic and knockout mice indicate that IL-1Ra is important in host defense against endotoxin-induced injury. IL-1Ra is produced by hepatic cells with the characteristics of an acute phase protein. Endogenous IL-1Ra is produced in numerous experimental animal models of diesease as well as in human autoimmune and chronic inflammatory diseases. The use of neutralizing anti-IL-1Ra antibodies has demonstrated that endogenous IL-1Ra is an important natural antiinflammatory protein in arthritis, colitis, and granulomatous pulmonary disease.
General readings
Capper., et al. (2004) Clin. Exp. Immunol. 138:348 Roberge CJ., et al. (1994) Journal of Immunology. 152: 5485-94 Carter DB., et al. (1990) Nature. 344: 633-7
Western Blot analysis of IL1RN expression in transfected 293T cell line (H00003557-T02) by IL1RN MaxPab polyclonal antibody.Lane 1: IL1RN transfected lysate(20.10 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of IL1RN expression in transfected 293T cell line (H00003557-T02) by IL1RN MaxPab polyclonal antibody.Lane 1: IL1RN transfected lysate(20.10 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of IL1RN expression in transfected 293T cell line (H00003557-T01) by IL1RN MaxPab polyclonal antibody.Lane 1: IL1RN transfected lysate(20.10 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged IL1RN is approximately 0.03ng/ml as a capture antibody.
Representative Standard Curve
Representative Standard Curve
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803396)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803396)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA803396)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IL1RN mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803422)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC202447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC218518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
ELISA: IL1RA antagonist Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1RN (RC218518, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1RN.
ELISA: IL1RA antagonist Antibody
To detect Human IL-1RA by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA.
To detect Human IL-1RA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1RA (TA328203) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA.
To detect Human IL-1RA by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (TA328202) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
To detect Human IL-1RA by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (TA328202) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-1RA.
IL1 Receptor antagonist antibody detects IL1RN protein by Western blot analysis. A. 30 ug Raji whole cell lysate/extract. 15 % SDS-PAGE. IL1 Receptor antagonist antibody (TA308778) dilution: 1:1000
IL1 Receptor antagonist antibody detects IL1RN protein by Western blot analysis. A. 30 ug Neuro2A whole lysate/extract. B. 30 ug C8D30 whole cell lysate/extract. C. 30 ug NIH-3T3 whole cell lysate/extract. D. 30 ug Raw264.7 whole cell lysate/extract. E. 3
IL1 Receptor antagonist antibody detects IL1RN protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. 15 % SDS-PAGE. IL1 Receptor antagonist antibody (TA308778) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist(TA308778) antibody at 1:500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist (TA308778) antibody at 1:200 dilution.
Western blot analysis of IL1RN (arrow) using rabbit polyclonal IL1RN Antibody (Center) (Cat. #TA324842). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2) (Origene Technologies).
IL1RN Antibody (Center) (RB18901) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL1RN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of IL1RN Antibody (Center)(Cat#TA324842) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IL1RN Antibody (Center) (Cat. #TA324842) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of extracts of various cell lines, using IL1RN antibody.
Immunohistochemistry of paraffin-embedded human epididymis using IL1RN antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL1RN antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using IL1RN antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of MCF7 cell using IL1RN antibody. Blue: DAPI for nuclear staining.
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (IL1RN Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using (IL1RN Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308778 | IL1RN / IL1RA antibody |
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| Rabbit Polyclonal antibody to IL1ra antagonist (interleukin 1 receptor antagonist) | |||||||
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Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +4 additional images |
Highly validated antibody! |
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| TA327265 | IL1RN / IL1RA antibody |
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| IL1RN Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody! |
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| TA803396 | IL1RN / IL1RA antibody |
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| IL1RN mouse monoclonal antibody, clone OTI3B1 (formerly 3B1) | |||||||
 |
Mouse | IgG2b | OTI3B1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +3 additional images |
Highly validated antibody!
|
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| TA803422 | IL1RN / IL1RA antibody |
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| IL1RN mouse monoclonal antibody, clone OTI2B1 (formerly 2B1) | |||||||
 |
Mouse | IgG2b | OTI2B1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +24 additional images |
Highly validated antibody!
|
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| TA324842 | IL1RN / IL1RA antibody |
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| Rabbit polyclonal IL1RN Antibody (Center) | |||||||
 |
Rabbit | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
| +3 additional images |
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| TA328202 | IL1RN / IL1RA antibody |
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| Biotinylated Anti-Human IL-1RA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
50 µg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA328203 | IL1RN / IL1RA antibody |
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| Anti-Human IL-1RA Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€275.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA350085 | IL1RN / IL1RA antibody |
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| Rabbit Polyclonal Anti-IL1RN Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA590308 | IL1RN / IL1RA antibody |
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| Rabbit Polyclonal IL1RN/IL1RA Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA590309 | IL1RN / IL1RA antibody |
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| Rabbit Polyclonal IL1RN/IL1RA Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | E, WB |
0.1 mg /
€409.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA803293 | IL1RN / IL1RA antibody |
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| IL1RN mouse monoclonal antibody, clone OTI2D9 (formerly 2D9) | |||||||
 |
Mouse | IgG2b | OTI2D9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803294 | IL1RN / IL1RA antibody |
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| IL1RN mouse monoclonal antibody, clone OTI2D5 (formerly 2D5) | |||||||
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Mouse | IgG2a | OTI2D5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803295 | IL1RN / IL1RA antibody |
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| IL1RN mouse monoclonal antibody, clone OTI5C5 (formerly 5C5) | |||||||
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Mouse | IgG2a | OTI5C5 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803483 | IL1RN / IL1RA antibody |
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| Purified IL1RN mouse monoclonal antibody, clone OTI2D2 (formerly 2D2) | |||||||
 |
Mouse | IgG1 | OTI2D2 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803484 | IL1RN / IL1RA antibody |
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| IL1RN mouse monoclonal antibody, clone OTI4E8 (formerly 4E8) | |||||||
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Mouse | IgG1 | OTI4E8 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AM20814PU-L | IL1RN / IL1RA antibody |
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Mouse | IgG1 | KT6 | Aff - Purified | Hu | E |
1 mg /
€1,240.00
|
| OriGene Technologies GmbH | |||||||
| AM20814PU-N | IL1RN / IL1RA antibody |
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|
Mouse | IgG1 | KT6 | Aff - Purified | Hu | E |
0.2 mg /
€540.00
|
| OriGene Technologies GmbH | |||||||
| AM20814PU-S | IL1RN / IL1RA antibody |
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|
Mouse | IgG1 | KT6 | Aff - Purified | Hu | E |
0.1 mg /
€390.00
|
| OriGene Technologies GmbH | |||||||
| AM26365PU-N | IL1RN / IL1RA (IL-1ra3) antibody |
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|
Mouse | IgG1 | 2D11 | Purified | Hu | C, E, WB |
0.1 mg /
€390.00
|
| OriGene Technologies GmbH | |||||||
| AP05027BT-N | IL1RN / IL1RA antibody |
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|
Rabbit | IgG | Biotin | Hu | E |
0.2 mg /
€470.00
|
|
| OriGene Technologies GmbH | |||||||
