ILF2 antibody
Principal name
ILF2 antibody
Alternative names for ILF2 antibody
ILF-2, NF45, NF-45, Interleukin enhancer-binding factor 2, Nuclear factor of activated T-cells 45 kDa
Ncbi ID
9606, NP_004506, NP_004506.2, NP_080650.1, NM_026374, NM_001047886, NM_004515
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of ILF2 antibody
- Kit Component:
- - KN308302G1, Ilf2 gRNA vector 1 in pCas-Guide vector
- KN308302G2, Ilf2 gRNA vector 2 in pCas-Guide vector
- KN308302D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Wang J, Rao S, Chu J, Shen X, Levasseur DN, Theunissen TW, Orkin SH. A protein interaction network for pluripotency of embryonic stem cells. Nature. 2006 Nov 16;444(7117):364-8. Epub 2006 Nov 8.
PMID: 17093407
Detection limit for recombinant GST tagged ILF2 is approximately 1ng/ml as a capture antibody.
ILF2 polyclonal antibody (A01), Lot # 050928JC01 Western Blot analysis of ILF2 expression in SJCRH30 ( Cat # L027V1 ).
Human kidney; ILF2 antibody - C-terminal region (ARP35731_T100) in Human kidney cells using Immunohistochemistry
Human Heart; . Rabbit Anti-ILF2 Antibody. . Catalog Number:. ARP35731 . Paraffin Embedded Tissue: Human cardiac cell . Cellular Data: Epithelial cells of renal tubule. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X
Jurkat; Host: Rabbit. Target Name:ILF2. Sample Tissue:Jurkat. Lane A: Primary Antibody . Lane B: Primary Antibody + Blocking Peptide . Primary Antibody Concentration:2.5ug/mL. Peptide Concentration: 2.0ug/mL. Lysate Quantity: 25ug/lane. Gel Concentration: 12%.; ILF2 antibody - C-terminal region (ARP35731_T100) in Jurkat cells using Western Blot
Human Jurkat; WB Suggested Anti-ILF2 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; ILF2 antibody - C-terminal region (ARP35731_T100) in Human Jurkat cells using Western Blot
Human MCF-7; . Sample Type: MCF-7 WT a whole cell lysates (110UG). Primary Dilution: 1.5mg/mL . Secondary Antibody: LI-COR Donkey anti-rabbit. Secondary Dilution: 1:10,000. . ILF2 is supported by BioGPS gene expression data to be expressed in MCF7.; ILF2 antibody - C-terminal region (ARP35731_T100) in Human MCF-7 cells using Western Blot
ILF2 polyclonal antibody ( Cat # PAB7568 ) ( 2.5 µg/ml ) staining of parental and stable knock-down cell line ( fixed by 4% formaldehyse, permealized by Triton X100 ) . Primary incubation was 1.5 hour. Detected by fluorescence.
ILF2 polyclonal antibody ( Cat # PAB7568 ) ( 0.3 µg/ml ) staining of human tonsil lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Immunoperoxidase of purified MaxPab antibody to ILF2 on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to ILF2 on HeLa cell. [antibody concentration 10 ug/ml]
ILF2 MaxPab polyclonal antibody. Western Blot analysis of ILF2 expression in human pancreas.
Western Blot analysis of ILF2 expression in transfected 293T cell line (H00003608-T01) by ILF2 MaxPab polyclonal antibody.Lane 1: ILF2 transfected lysate(42.9 KDa).Lane 2: Non-transfected lysate.
ILF2 MaxPab polyclonal antibody. Western Blot analysis of ILF2 expression in Jurkat.
Immunoprecipitation of ILF2 transfected lysate using anti-ILF2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ILF2 purified MaxPab mouse polyclonal antibody (B01P) (H00003608-B01P).
Immunohistochemistry Image: Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry Image: Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot Image: Antibody (0.3 µg/ml) staining of Human Tonsil lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-ILF2mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500674, Dilution 1:50)
Anti-ILF2 mouse monoclonal antibody (TA500674) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ILF2(RC201751).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ILF2 (RC201751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ILF2.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ILF2 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ILF2 monoclonal antibody.
TA303236 (0.3µg/ml) staining of Human Tonsil lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Host: Rabbit; Target Name: Ilf2; Sample Tissue: Mouse Heart lysates; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-ILF2 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:62500; Positive Control: Jurkat cell lysate
Host: Rabbit; Target Name:ILF2; Sample Tissue:Jurkat; Lane A: Primary Antibody; Lane B: Primary Antibody + Blocking Peptide ; Primary Antibody Concentration:2.5ug/mL; Peptide Concentration: 2.0ug/mL; Lysate Quantity: 25ug/lane; Gel Concentration: 12%
Sample Type: MCF-7 WT a whole cell lysates (110UG); Primary Dilution: 1.5mg/mL ; Secondary Antibody: LI-COR Donkey anti-rabbit; Secondary Dilution: 1:10,000; ; ILF2 is supported by BioGPS gene expression data to be expressed in MCF7
Human kidney
Rabbit Anti-ILF2 Antibody; Paraffin Embedded Tissue: Human cardiac cell; Cellular Data: Epithelial cells of renal tubule; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Host: Rabbit; Target Name: ILF2; Sample Tissue: HepG2 Whole cell lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Cited (1)
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP23050PU-N | ILF2 (355-367) antibody |
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Goat | Aff - Purified | Bov, Can, Elephant, Eq, Hu, Mky, Ms, Por, Rb, Rt | E, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
 +2 additional images |
Published recently! |
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| TA500674 | ILF2 antibody |
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| anti-ILF2 mouse monoclonal antibody, clone OTI6F1 (formerly 6F1) | |||||||
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Mouse | IgG1 | OTI6F1 | Purified | Can, Hu | ICC/IF, P, WB |
0.1 ml /
€379.00
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| OriGene Technologies, Inc. | |||||||
| +4 additional images |
Highly validated antibody!
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| TA303236 | ILF2 antibody |
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| Goat Polyclonal Antibody against ILF2 | |||||||
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Goat | Aff - Purified | Can, Hu, Ms, Rt | E, P, WB |
0.1 mg /
€360.00
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| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301751 | ILF2 |
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| Recombinant protein of human interleukin enhancer binding factor 2, 45kDa (ILF2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
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|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206085L2V | Lenti ORF particles, Ilf2 (GFP-tagged) - Mouse interleukin enhancer binding factor 2 (Ilf2), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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|
| OriGene Technologies, Inc. | ||
| MR206085L1V | Lenti ORF particles, Ilf2 (Myc-DDK-tagged) - Mouse interleukin enhancer binding factor 2 (Ilf2), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203617L2V | Lenti ORF particles, Ilf2 (GFP-tagged ORF) - Rat interleukin enhancer binding factor 2 (Ilf2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,140.00
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| OriGene Technologies, Inc. | ||
| RR203617L1V | Lenti ORF particles, Ilf2 (Myc-DDK-tagged ORF) - Rat interleukin enhancer binding factor 2 (Ilf2), 200 uL, >10^7 TU/mL |
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200 µl /
€1,020.00
|
|
| OriGene Technologies, Inc. | ||
