Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » IMPDH2 antibody

IMPDH2 antibody

(reset)

Principal name

IMPDH2 antibody

Alternative names for IMPDH2 antibody

IMPD2, Inosine-5'-monophosphate dehydrogenase 2, IMP dehydrogenase 2, EC=1.1.1.205, IMPDH-II, IMPD-2

SwissProt ID

P12268 (Human), P24547 (Mouse), Q3SWY3 (Bovin)

Gene ID

3615 (IMPDH2), 23918 (Impdh2)

Ncbi ID

9606, NP_000875, NM_000884, NP_000875.2, NP_035960.2

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of IMPDH2 antibody

IMPDH2 is the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. It catalyzes the NAD-dependent oxidation of inosine-5\'-monophosphate into xanthine-5\'-monophosphate, which is then converted into guanosine-5\'-monophosphate. IMPDH2 is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5\'-monophosphate into xanthine-5\'-monophosphate, which is then converted into guanosine-5\'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Fellenberg J, Bernd L, Delling G, Witte D, Zahlten-Hinguranage A.
Prognostic significance of drug-regulated genes in high-grade osteosarcoma.
Mod Pathol. 2007 Oct;20(10):1085-94. Epub 2007 Jul 27.
PMID: 17660802 Comments: Osteosarcoma.

H00003615-M01 - IMPDH2
Western Blot analysis of IMPDH2 expression in transfected 293T cell line by IMPDH2 monoclonal antibody (M01), clone 1E12-B6.Lane 1: IMPDH2 transfected lysate(55.8 KDa).Lane 2: Non-transfected lysate.
H00003615-M01 - IMPDH2
Western blot analysis of IMPDH2 over-expressed 293 cell line, cotransfected with IMPDH2 Validated Chimera RNAi ( Cat # H00003615-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IMPDH2 monoclonal antibody (M01), clone 1E12-B6 (Cat # H00003615-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
H00003615-M01 - IMPDH2
Detection limit for recombinant GST tagged IMPDH2 is approximately 0.3ng/ml as a capture antibody.
H00003615-D01P - IMPDH2
Western Blot analysis of IMPDH2 expression in transfected 293T cell line (H00003615-T01) by IMPDH2 MaxPab polyclonal antibody.Lane 1: IMPDH2 transfected lysate(55.80 KDa).Lane 2: Non-transfected lysate.
ARP54366_P050 - IMPDH2
Human HepG2; WB Suggested Anti-IMPDH2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; IMPDH2 antibody - C-terminal region (ARP54366_P050) in Human HepG2 cells using Western Blot
H00003615-B01P - IMPDH2
Western Blot analysis of IMPDH2 expression in transfected 293T cell line (H00003615-T02) by IMPDH2 MaxPab polyclonal antibody.Lane 1: IMPDH2 transfected lysate(56.54 KDa).Lane 2: Non-transfected lysate.
AP17504PU-N - IMPDH2
Western blot analysis of IMPDH2 Antibody (Center) (AP17504PU-N) in NCI-H460,Hela,MCF7,A2058 cell line lysates(35ug/lane). IMPDH2 (arrow) was detected using the purified Pab.
ARP54365_P050 - IMPDH2
Human Hep-2; . Sample Type :. Human Hep-2 cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit-Alexa Fluor 568. Secondary Antibody Dilution :. 1:400. Color/Signal Descriptions :. Red: IMPDH2 Blue: DAPI. Gene Name :. IMPDH2. Submitted by :. S. John Calise, Edward Chan Lab, University of Florida; IMPDH2 antibody - N-terminal region (ARP54365_P050) in Human Hep-2 cells using Immunohistochemistry
ARP54365_P050 - IMPDH2
Human 721_B; WB Suggested Anti-IMPDH2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: 721_B cell lysate. IMPDH2 is supported by BioGPS gene expression data to be expressed in 721_B.; IMPDH2 antibody - N-terminal region (ARP54365_P050) in Human 721_B cells using Western Blot
AR50099PU-N - IMPDH2
TP302977 - IMPDH2
TA305796 - IMPDH2
TA305796 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA332628 - IMPDH2
Western blot analysis of extracts of various cell lines, using IMPDH2 antibody.
TA332628 - IMPDH2
Immunofluorescence analysis of HeLa cell using IMPDH2 antibody.
TA336212 - IMPDH2
WB Suggested Anti-IMPDH2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: 721_B cell lysate. IMPDH2 is supported by BioGPS gene expression data to be expressed in 721_B
TA336213 - IMPDH2
WB Suggested Anti-IMPDH2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate
TA350091 - IMPDH2
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A549 cells, Primary antibody: (IMPDH2 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes
TA350091 - IMPDH2
The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using (IMPDH2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
TA350091 - IMPDH2
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (IMPDH2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IMPDH2 antibody
  Goat Anti-IMPDH2 Antibody  
TA305796 (1ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

IMPDH2 antibody
  Rabbit anti-IMPDH2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using IMPDH2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

IMPDH2 antibody
  Rabbit Polyclonal Anti-IMPDH2 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A549 cells, Primary antibody: (IMPDH2 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

IMPDH2 antibody
Western blot analysis of IMPDH2 Antibody (Center) (AP17504PU-N) in NCI-H460,Hela,MCF7,A2058 cell line lysates(35ug/lane). IMPDH2 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

IMPDH2
  Recombinant protein of human IMP (inosine monophosphate) dehydrogenase 2 (IMPDH2)  
IMPDH2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

IMPDH2 overexpression lysate
IMPDH2 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

6 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (4)
Target
  1. IMPDH2 (5)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (3)
  2. Goat (1)
Images
  1. 1 image (5)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Immunofluorescence (IF) (1)
  3. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (6)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn