ING1 antibody
Principal name
ING1 antibody
Alternative names for ING1 antibody
Inhibitor of growth protein 1, ING-1
Ncbi ID
NP_005528.3, NP_937862, 9606, NP_005528, NM_198219, NP_937861, NM_198218, NM_005537, NP_036049.2, NM_011919, NM_198217
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP)
Background of ING1 antibody
- Kit Component:
- - KN308326G1, Ing1 gRNA vector 1 in pCas-Guide vector
- KN308326G2, Ing1 gRNA vector 2 in pCas-Guide vector
- KN308326D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Campos,E.I., Chin,M.Y., Kuo,W.H. and Li,G. (2004) Biological functions of the ING family tumor suppressors. Cell. Mol. Life Sci. 61 (19-20). 2597-2613.
2. Cheung,K.J. Jr. and Li,G. (2002) p33(ING1) enhances UVB-induced apoptosis in melanoma cells. Exp. Cell Res. 279 (2), 291-298.
3. Vieyra,D. et al (2002) Human ING1 proteins differentially regulate histone acetylation. J. Biol. Chem. 277 (33), 29832-29839.
4. Nagashima,M. et al (2001) DNA damage-inducible gene p33ING2 negatively regulates cell proliferation through acetylation of p53. Proc. Natl. Acad. Sci. U.S.A. 98 (17), 9671-9676.
5. Shinoura,N. et al (1999) Adenovirus-mediated transfer of p33ING1 with p53 drastically augments apoptosis in gliomas. Cancer Res. 59 (21), 5521-5528.
6. Garkavtsev,I., Grigorian,I.A., Ossovskaya,V.S., Chernov,M.V., Chumakov,P.M. and Gudkov,A.V. (1998) The candidate tumour suppressor p33ING1 cooperates with p53 in cell growth control. Nature 391 (6664), 295-298.
7. Helbing,C.C., Veillette,C., Riabowol,K., Johnston,R.N. and Garkavtsev,I. (1997) A novel candidate tumor suppressor, ING1, is involved in the regulation of apoptosis. Cancer Res. 57 (7), 1255-1258.
8. Garkavtsev,I. and Riabowol,K. (1997) Extension of the replicative life span of human diploid fibroblasts by inhibition of the p33ING1 candidate tumor suppressor.
Mol. Cell. Biol. 17 (4), 2014-2019.
9. Garkavtsev,I., Demetrick,D. and Riabowol,K. (1997) Cellular localization and chromosome mapping of a novel candidate tumor suppressor gene (ING1).
Cytogenet. Cell Genet. 76 (3-4), 176-178.
10. Garkavtsev,I., Kazarov,A., Gudkov,A. and Riabowol,K. (1996) Suppression of the novel growth inhibitor p33ING1 promotes neoplastic transformation. Nat. Genet. 14 (4), 415-420.
Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex.
Human 721_B; Host:Rabbit. Target Name:ING1. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. ING1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.; ING1 antibody - N-terminal region (ARP50511_P050) in Human 721_B cells using Western Blot
Human HeLa; WB Suggested Anti-ING1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate.. ING1 is strongly supported by BioGPS gene expression data to be expressed in HeLa.; ING1 antibody - N-terminal region (ARP50511_P050) in Human HeLa cells using Western Blot
Human; . Lanes: 1: 30ug HeLa lysate, 2: 30ug HFF lysate, 3: 30ug U2OS lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:5000. Gene Name: ING1. Submitted by: Dr. Hyung Suk Oh, Knipe Lab, Harvard Medical School.; ING1 antibody - C-terminal region (ARP50512_T100) in Human cells using Western Blot
Human HepG2; WB Suggested Anti-ING1 Antibody Titration: 2.5ug/ml. Positive Control: HepG2 cell lysate. There is BioGPS gene expression data showing that ING1 is expressed in HepG2.; ING1 antibody - C-terminal region (ARP50512_T100) in Human HepG2 cells using Western Blot
Immunofluorescence of purified MaxPab antibody to ING1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ING1 expression in transfected 293T cell line (H00003621-T01) by ING1 MaxPab polyclonal antibody.Lane 1: ING1 transfected lysate(30.69 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ING1 expression in transfected 293T cell line (H00003621-T02) by ING1 MaxPab polyclonal antibody.Lane 1: ING1 transfected lysate(31.90 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of ING1 transfected lysate using anti-ING1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ING1 MaxPab mouse polyclonal antibody (B01) (H00003621-B01).
Western Blot analysis of ING1 expression in transfected 293T cell line (H00003621-T02) by ING1 MaxPab polyclonal antibody.Lane 1: ING1 transfected lysate(31.90 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ING1 is approximately 0.1ng/ml as a capture antibody.
ING1 monoclonal antibody (M02A), clone 2E5 Western Blot analysis of ING1 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged ING1 is approximately 0.03ng/ml as a capture antibody.
ING1 monoclonal antibody (M05), clone 2F9 Western Blot analysis of ING1 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged ING1 is approximately 0.1ng/ml as a capture antibody.
ING1 monoclonal antibody (M05), clone 2F9 Western Blot analysis of ING1 expression in MCF-7 ( Cat # L046V1 ).
Detection limit for recombinant GST tagged ING1 is approximately 0.1ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ING1 is approximately 0.03ng/ml as a capture antibody.
Detection limit for recombinant GST tagged ING1 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis is shown using ING1 polyclonal antibody ( Cat # PAB10082 ) to detect with purified recombinant GST tagged human ING1b ( Lane 1 ) and ING1c protein ( Lane 2 ). No reactivity is seen against GST or other ING proteins ( not shown ). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody ( not shown ). The blot was incubated with a 1 : 1,000 dilution of the antibody at room temperature followed by detection using standard techniques.Personal communication Xiaolan Feng, U. Calgary.
Immunohistochemical staining of human gall bladder shows nuclear and cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus, nucleoli & plasma membrane.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ING1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ING1 (RC210888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ING1.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: ING1 Antibody
Western blot analysis of p33 ING1 in mouse brain extract using the antibody
WB analysis using Anti-p33 ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This WB shows reactivity with purified recombinant GST tagged human ING1b (lane 1) and ING1c protein (lane 2). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at RT.
WB Suggested Anti-ING1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate.ING1 is strongly supported by BioGPS gene expression data to be expressed in HeLa
Host: Rabbit; Target Name: ING1; Sample Tissue: Human 721_B; Antibody Dilution: 1.0 ug/ml ING1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Lanes: 1: 30 ug HeLa lysate, 2: 30 ug HFF lysate, 3: 30 ug U2OS lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 5000; Gene Name: ING1; Submitted by: Dr. Hyung Suk Oh, Knipe Lab, Harvard Medi
WB Suggested Anti-ING1 Antibody Titration: 2.5 ug/ml; Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that ING1 is expressed in HepG2
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: Hela cells, mouse brain tissue, Jurkat cells, Raw264.7 cells, Primary antibody: (ING1 Antibody) at dilution 1/267, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 second
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA590316 | ING1 antibody |
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| Rabbit Polyclonal ING1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Mky, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€409.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| TA319418 | ING1 antibody |
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| Goat polyclonal anti-p33 ING1 antibody | |||||||
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Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€515.00
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| OriGene Technologies, Inc. | |||||||
| TA326481 | ING1 antibody |
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| Rabbit Polyclonal Anti-p33 ING1 Antibody | |||||||
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Rabbit | IgG | Serum | Hu, Ms | E, ICC/IF, WB |
0.4 ml /
€400.00
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| OriGene Technologies, Inc. | |||||||
| TA350698 | ING1 antibody |
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| Rabbit Polyclonal Anti-ING1 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
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| OriGene Technologies, Inc. | |||||||
| AP07596PU-N | ING1 antibody |
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Goat | Aff - Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€425.00
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| OriGene Technologies GmbH | |||||||
| R1608CP | ING1 Control Peptide |
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Purified |
50 µg /
€205.00
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| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP317763 | ING1 (transcript variant 1) |
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| Recombinant protein of human inhibitor of growth family, member 1 (ING1), transcript variant 1 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||
| TP760507 | ING1 (full length, N-term HIS tag, transcript variant 2) |
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| Purified recombinant protein of Human inhibitor of growth family, member 1 (ING1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
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Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
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| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403677 | ING1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY404951 | ING1 overexpression lysate |
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0.1 mg /
€295.00
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| OriGene Technologies, Inc. | ||
| LY430724 | ING1 overexpression lysate |
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0.1 mg /
€315.00
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| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC217763L2V | Lenti ORF particles, ING1 (mGFP-tagged) - Human inhibitor of growth family, member 1 (ING1), transcript variant 1, 200 uL, >10^7 TU/mL |
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200 µl /
€1,020.00
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| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR225175L2V | Lenti ORF particles, Ing1 (GFP-tagged) - Mouse inhibitor of growth family, member 1 (Ing1), 200 uL, >10^7 TU/mL |
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200 µl /
€930.00
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| OriGene Technologies, Inc. | ||