ING5 antibody
Principal name
ING5 antibody
Alternative names for ING5 antibody
Inhibitor of growth protein 5, p28ING5
Ncbi ID
NP_115705.2, NP_115705, XP_001126273, NM_032329, NP_079730.1, BC064674, NM_025454
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of ING5 antibody
- Kit Component:
- - KN308330G1, Ing5 gRNA vector 1 in pCas-Guide vector
- KN308330G2, Ing5 gRNA vector 2 in pCas-Guide vector
- KN308330D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ullah, M., et al. Mol. Cell. Biol. 28(22):6828-6843(2008)
Champagne, K.S., et al. Proteins 72(4):1371-1376(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Immunofluorescence of purified MaxPab antibody to ING5 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ING5 expression in transfected 293T cell line (H00084289-T01) by ING5 MaxPab polyclonal antibody.Lane 1: ING5 transfected lysate(26.4 KDa).Lane 2: Non-transfected lysate.
Western blot analysis is shown using ING5 polyclonal antibody ( Cat # PAB10079 ) to detect over expressed Human ING5 present in HeLa cell nuclear extracts.This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4on the same membrane (lane 2). A mock purification is shown inlane 1. Comparison to a molecular weight marker (not shown)indicates a single band of ~45.0 kDa corresponding to theexpected molecular weight for the recombinant protein. The blotwas incubated with a 1:500 dilution of the antibody at roomtemperature followed by detection using chemiluminescencereagent with a 5-min exposure time. Other detection systems willyield similar results. Personal communication Jacques Cote.
Western blot analysis is shown using ING5 polyclonal antibody ( Cat # PAB10079 ) to detect with purified recombinant human ING5 protein.Indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.The primary antibody diluted to 1 : 1,500.Followed by washes and reaction with a 1 : 20,000 dilution of IRDyeTM800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.IRDyeTM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
Human OVCAR-3; WB Suggested Anti-ING5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: OVCAR-3 cell lysate; ING5 antibody - N-terminal region (ARP50925_P050) in Human OVCAR-3 cells using Western Blot
Western blot analysis of ING5 Antibody (Center) Cat.-No AP52210PU-N in A2058 cell line lysates (35ug/lane). ING5 (arrow) was detected using the purified Pab.
ING5 antibody [N2C1], Internal detects ING5 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. 12 % SDS-PAGE. ING5 antibody [N2C1], Internal (TA308527) dilution: 1:1000
WB analysis using Anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This WB shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification in lane 1. The blot was incubated with a 1:500 dilution of the antibody at RT.
WB analysis using Anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500.
WB Suggested Anti-ING5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: OVCAR-3 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308527 | ING5 antibody |
||||||
| Rabbit polyclonal antibody to ING5 (inhibitor of growth family, member 5) | |||||||
![ING5 antibody [N2C1], Internal detects ING5 protein by Western blot analysis. A. 30 ug Jurkat whole cell lysate/extract. 12 % SDS-PAGE. ING5 antibody [N2C1], Internal (TA308527) dilution: 1:1000](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/GTX102482-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Hu | WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA319227 | ING5 antibody |
||||||
| Goat polyclonal anti-p28 ING5 antibody | |||||||
 |
Goat | Aff - Purified | Hu, Mky | E, WB |
0.1 mg /
€515.00
|
||
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
| AP52210PU-N | ING5 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| R1605CP | ING5 Control Peptide |
||||||
 |
Purified |
50 µg /
€205.00
|
|||||
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760410 | ING5 (full length, N-term HIS tag) |
||||
| Purified recombinant protein of Human inhibitor of growth family, member 5 (ING5), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug | |||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€205.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY410205 | ING5 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR201124L2V | Lenti ORF particles, Ing5 (GFP-tagged) - Mouse inhibitor of growth family, member 5 (cDNA clone MGC:66742 IMAGE:6400183), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR201124L1V | Lenti ORF particles, Ing5 (Myc-DDK-tagged) - Mouse inhibitor of growth family, member 5 (cDNA clone MGC:66742 IMAGE:6400183), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||
