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Insulin antibody

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Principal name

Insulin antibody

Alternative names for Insulin antibody

INS

SwissProt ID

P01308 (Human), P01310 (Horse), P01311 (Rabit), P01315 (Pig), P01317 (Bovin), P01318 (Sheep), P01321 (Canfa), P01329 (Cavpo), P06306 (Felca), P67970 (Chick)

Gene ID

3630 (INS), 397415

Ncbi ID

NP_000198.1, NP_000198, 9606, 9823, NP_001103242.1, NM_000207

Available reactivities

Bov (Bovine), Hu (Human), Por (Porcine), Rb (Rabbit), Rt (Rat), Can (Canine), Eq (Equine), Fe (Feline), GP (Guinea Pig), Sh (Sheep), Ms (Mouse), Ft (Ferret), Hst (Hamster), Deer, Mky (Monkey)

Available hosts

Mouse, Rabbit, Guinea Pig, Sheep, Goat

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Paraffin Sections (P), Functional assay (FN), Radioimmunoassay (R), ELISA (capture) (E(capture)), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), Dot blot (Dot), Immunodiffusion (ID)

Background of Insulin antibody

Proinsulin is synthesized as a single chain, 110 amino acid (aa) preproprecursor that contains a 24 aa signal sequence and an 86 aa proinsulin propeptide. Following removal of the signal peptide, the proinsulin peptide undergoes further proteolysis to generate mature insulin, a 51 aa disulfidelinked dimer that consists of a 30 aa B chain (aa 2554) bound to a 21 aa A chain (aa 90110). The 34 aa intervening peptide (aa 5589)that connects the B and A chains is termed the Cpeptide.

Human proinsulin shares 84% and 80% aa sequence identity with rat and bovine proinsulin, respectively. Most of the sequence variation between species occurs in the region of the Cpeptide. This peptide generates a structural conformation that allows for the correct formation of the intrachain disulphide bonds . Insulin is a molecule that facilitates the cellular uptake of glucose. This is accomplished by regulating the appearance of membrane glucose transporters. Low insulin levels or lack of insulin are associated with type 2 and type 1 diabetes mellitus, respectively. These conditions are associated with an increased risk for microvascular complications such as retinopathy, nephropathy, and peripheral neuropathy . Proinsulin also circulates, but its physiologic role is less well understood. It does possess about 25% of the activity of mature insulin, but it would seem unlikely to be a natural substitute for insulin . In type 2 diabetes, an elevated proinsulin to insulin ratio in the circulation is a well known abnormality . Perhaps this abnormality represents either compromised proteolytic processing or a general inability to process increased levels of insulin precursor . In any event, proinsulin will stimulate amylin secretion by .cells, and amyloid formation in pancreatic islets that promotes decreased . cell function . Studies also suggest that fasting serum proinsulin may be a better predictor of future type 2 diabetes than fasting insulin levels in obese children .

General readings

Bell, G.I. et al. (1980) Nature 284:26.

Barbetti, F. et al. (1990) J. Clin. Endocrinol. Metab. 71:164.

Forst, T. et al. (2008) Exp. Diabetes Res. 2008:176245.

Steffes, M.W. et al. (2003) Diabetes Care 26:832.

Roder, M.E. et al. (1999) Diabetes Care 22:609.

Porte, D. Jr. (1991) Diabetes 40:166.

Gordon, P. et al. (1974) Diabetologia 34:483.

Saad, M.F. et al. (1990) J. Clin. Endocrinol. Metab. 70:1247.

Roder, M.E. et al. (1995) J. Clin. Endocrinol. Metab. 80:2359.

Dworacka, M. et al. (2006) Int. J. Clin. Pharmacol. Ther. 44:14.

Kamoda, T. et al. (2006) Diabetes Obes. Metab. 8:192.

BM504S - Insulin
Formalin fixed paraffin embedded human pancreas stained with Insulin antibody BM504S/BM504S-05
20056 - Insulin
Staining with Insulin Antibody 20056
BM508 - Insulin
Staining of Paraffin Embedded Human pancreas with Mouse anti Rat Insulin Antibody Cat.-No BM508 (Clone D3E7)
AM05475PU-N - Insulin
Formalin Fixed, Paraffin Embedded Human pancreas stained with Mouse anti Pig Insulin Antibody Cat.-No AM05475PU-N (Clone 2D11).
AM05475PU-N - Insulin
Formalin Fixed, Paraffin Embedded Human pancreas stained with Mouse anti Pig Insulin Antibody Cat.-No AM05475PU-N (Clone 2D11) following pronase digestion.
PAB2057 - Insulin
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with INS polyclonal antibody ( Cat # PAB2057 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
H00003630-M01 - Insulin
Western Western Blot detection of different antigens with INS monoclonal antibody, clone 3E7 ( Cat # H00003630-M01 ) , the results showed that this antibody only reacted with INS, not mature Insulin.Lane 1, Recombinant human INS ( 25 a.a. ~ 110 a.a., NP_000198 ) with N-terminal GST tag ( 35.5 KDa ) .Lane 2, GST protein ( 26 KDa ) .Lane 3, Mature human insulin from Sigma ( Cat # I2643 ) .
H00003630-M02 - Insulin
Western Blot detection of Western Blot detection of different antigens with INS monoclonal antibody, clone 7C8 ( Cat # H00003630-M02 ) , the results showed that this antibody only reacted with INS, not mature Insulin.Lane 1, Recombinant human INS ( 25 a.a. ~ 110 a.a., NP_000198 ) with N-terminal GST tag ( 35.5 KDa ) .Lane 2, GST protein ( 26 KDa ) .Lane 3, Mature human insulin from Sigma ( Cat # I2643 ) .
H00003630-M03 - Insulin
Western Blot detection of different antigens with INS monoclonal antibody, clone 5B5 ( Cat # H00003630-M03 ) , the results showed that this antibody only reacted with INS, not mature Insulin.Lane 1, Recombinant human INS ( 25 a.a. ~ 110 a.a., NP_000198 ) with N-terminal GST tag ( 35.5 KDa ) .Lane 2, GST protein ( 26 KDa ) .Lane 3, Mature human insulin from Sigma ( Cat # I2643 ) .
PAB8787 - Insulin
Western blot analysis using INS polyclonal antibody ( Cat # PAB8787 ) .
AP08860PU-N - Insulin
Figure 1. AP08860PU-N Insulin antibody staining of Formalin-Fixed Paraffin-Embedded Human Pancreas at 1/100, followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
AM08397HR-N - Insulin
AM08397HR-N Insulin Antibody stainig of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
MAB3849 - Insulin
Immunohistochemistry staining of human pancreas ( paraffin-embedded sections ) with C-peptide monoclonal antibody, clone C-PEP-01 ( Cat # MAB3849 ).
AM12123PU-N - Insulin
Immunohistochemistry of Human Pancreas (Left): Antigen retrieval was done with Tris-EDTA, pH 9.0 in pressure cooker for 20 minutes. Optimum staining of the islet cell was observed.Indirect ELISA (Right): Serial dilution of the recombinant insulin was done to check the affinity. The antibody used at 10 µg/ml can sensitively detect antigen up to 4 ng per well.
AM12123PU-N - Insulin
Western Blot Analysis: 10 µg of Insulin run on 12% SDS-PAGE. The pro-insulin antibody detected a band at ~6 kDa.
AP20659PU-N - Insulin
Immunohistochemistry analysis of Insulin Antibody (Cat.-No AP20659PU-N) in paraffin-embedded human pancreas tissue.
T-1313 - Insulin
T-1313 C-peptide (connecting peptide of proinsulin), mouse anti human, clone C-PEP-01 (ascites monoclonal IgG1, lyophilized)
AM20632PU-N - Insulin
Immunohistochemistry on Rat pancreas sections
AM26629AF-N - Insulin
 
Immunohistochemical detection of Insulin expressed in β cell in Langerhans islet in pancreas using AM26629AF-N
AM26629AF-N - Insulin
Western blot analysis of Insulin B chain from human recombinant Insulin using AM26629AF-N.
AM26629AF-N - Insulin
Immunoprecipitation of Insulin B chain from human recombinant Insulin with M 093-3 (1) or mouse IgG 1 (2) . After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26629AF-N.
AM50123PU-S - Insulin
Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM139). Note cytoplasmic staining of cells.
AM50124PU-S - Insulin
Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM531). Note cytoplasmic staining of cells.
AM33363PU-S - Insulin
Formalin-Paraffin Human pancreas stained with Insulin Antibody Cat.-No AM33363PU (Clone E2-E3+2D11-H5). Note cytoplasmic staining of cells.
CPA2974-100ul - Insulin
Western blot analysis of Insulin expression in A549 (A); mouse heart (B); rat liver (C) whole cell lysates.
CPA2975-100ul - Insulin
Western blot analysis of Insulin expression in A549 (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
CEK1245 - Human Insulin ELISA Kit
Representative Standard Curve
TP302701 - Insulin
TA319541 - Insulin
ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1?g of conjugate. The starting concentration of antibody in the dilution series was 10 ?g/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approx-imately 5ng/ml antibody or a 1:200,000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(p/n 610-103-121) and TMB substrate were used for detection.
TA319541 - Insulin
Dot blotting. Mab anti-Insulin antibody (clone 2D11.H5) to detect human insulin by dot blot. Each dot blot represents 1 µl of non-denatured human insulin at various dilutions starting at 1.0 µg/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] .
TA327695 - Insulin
Immunohistochemistry staining of Paraffin Pancreas tissue by Insulin antibody (dilution: 1:100 - 1:500; visualization of staining: Cytoplasmic)
TA347869 - Insulin
Immunohistochemistry analysis of Insulin antibody in paraffin-embedded human pancreas tissue.
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Insulin antibody
Formalin-Paraffin Human pancreas stained with Insulin Antibody Cat.-No AM33363PU (Clone E2-E3+2D11-H5). Note cytoplasmic staining of cells. Mouse IgG1 E2-E3+2D11-H5 (INS04+ INS05) Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €430.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Insulin antibody
Formalin-Paraffin Human pancreas stained with Insulin Antibody Cat.-No AM33363PU (Clone E2-E3+2D11-H5). Note cytoplasmic staining of cells. Mouse IgG1 E2-E3+2D11-H5 (INS04+ INS05) Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
20 µg / €240.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Insulin antibody
Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM139). Note cytoplasmic staining of cells. Mouse IgG1 SPM139 Purified Bov, Hu, Por C, F, ICC/IF, IP, P, WB
0.1 mg / €430.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Insulin antibody
Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM139). Note cytoplasmic staining of cells. Mouse IgG1 SPM139 Purified Bov, Hu, Por C, F, ICC/IF, IP, P, WB
20 µg / €240.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Insulin antibody
Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM531). Note cytoplasmic staining of cells. Mouse IgG1 SPM531 Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
0.1 mg / €430.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Insulin antibody
Formalin-Paraffin Human pancreas stained with Insulin Antibody (Clone SPM531). Note cytoplasmic staining of cells. Mouse IgG1 SPM531 Purified Bov, Hu, Por, Rb, Rt C, F, ICC/IF, IP, P, WB
20 µg / €240.00
  OriGene Technologies GmbH
 

OriGene recommends this product for Immunohistochemistry.

Insulin antibody
  Mouse monoclonal Insulin antibody  
ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1?g of conjugate. The starting concentration of antibody in the dilution series was 10 ?g/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approx-imately 5ng/ml antibody or a 1:200,000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(p/n 610-103-121) and TMB substrate were used for detection. Mouse IgG1 2D11.H5 Purified Hu, Por C, E, IP, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.
+1 additional image

Insulin antibody
  Rabbit Polyclonal Anti-Insulin Antibody  
Immunohistochemistry analysis of Insulin antibody in paraffin-embedded human pancreas tissue. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.

Insulin antibody
  Rabbit Polyclonal Anti-INS Antibody  
Insulin Rabbit IgG Purified Hu E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

Insulin antibody
Insulin Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt WB
0.6 mg / €320.00
  SICGEN

Insulin antibody
Insulin Mouse IgG1 D4B8 Purified C, E
0.2 mg / €460.00
  OriGene Technologies GmbH

Insulin antibody
Formalin Fixed, Paraffin Embedded Human pancreas stained with Mouse anti Pig Insulin Antibody Cat.-No AM05475PU-N (Clone 2D11). Mouse IgG1 2D11 Purified Bov, Hu, Por E, P
0.2 mg / €480.00
  OriGene Technologies GmbH
+1 additional image

Insulin antibody
AM08397HR-N Insulin Antibody stainig of Formalin-Fixed, Paraffin-Embedded Human Pancreas at 10 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 8E2 HRP Hu E, P
50 µg / €425.00
  OriGene Technologies GmbH

Insulin antibody
Immunohistochemistry on Rat pancreas sections Mouse IgG1 ISL-8J Purified Hu, Ms, Rt P
0.1 mg / €390.00
  OriGene Technologies GmbH

Insulin antibody
  Immunohistochemical detection of Insulin expressed in β cell in Langerhans islet in pancreas using AM26629AF-N Mouse IgG1 1C5A Azide Free Hu IP, P, WB
0.1 mg / €375.00
  OriGene Technologies GmbH
+2 additional images

Insulin antibody
Insulin Mouse IgG1 E2-E3 Purified Bov, Hu, Por E, P
0.1 mg / €310.00
  OriGene Technologies GmbH

Insulin antibody
Insulin Guinea Pig Serum Bov, Deer E, R
0.1 ml / €640.00
  OriGene Technologies GmbH

Insulin antibody
Insulin Guinea Pig Serum Bov, Deer E, R
20 µl / €210.00
  OriGene Technologies GmbH

Insulin (57-87) antibody
Insulin Rabbit Serum Hu, Ms C, E, ICC/IF, P, R, WB
0.1 ml / €220.00
  OriGene Technologies GmbH

Insulin (57-87) antibody
Insulin Rabbit Serum Hu, Ms C, E, ICC/IF, P, R, WB
20 µl / €120.00
  OriGene Technologies GmbH

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47 products - FILTER YOUR RESULTS!

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Service
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