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Interleukin-25 / IL25 antibody

Principal name

Interleukin-25 / IL25 antibody

Alternative names for Interleukin-25 / IL25 antibody

IL-25, IL17E, IL-17E, Interleukin-17E

SwissProt ID

Q9H293 (Human)

Gene ID

64806 (IL25)

Ncbi ID

NP_073626.1, 9606, NP_073626, D3ZLB1, NM_172314, NM_022789, NP_758525

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C)

Background of Interleukin-25 / IL25 antibody

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C and IL-17E. IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis, multiple sclerosis, and promotion of tumor growth. IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al and Lee, et al. and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.
The IL17 proteins are a family of potent cytokines that act to induce proinflammatory responses. Studies have shown that IL17E binds strongly to IL17RB. Receptor binding of ligand has been shown to lead to the activation of nuclear factor kappa-B and production of IL8. Exposure of mice to IL17 resulted in a Th-2 like response characterized by increased serum IgE, IgG1 and IgA levels, blood eosinophilia, increased lymphocytes and neutrophils, and pathological changes in the tissues that included eosinophilic infiltrates, increased mucus production, B-lymphocyte hyperplasia and epithelial cell hyperplasia/hypertrophy.

General readings

Xu, G., et al. Allergy 65(5):581-589(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Goswami, S., et al. Nat. Immunol. 10(5):496-503(2009)
Caruso, R., et al. Blood 113(15):3512-3519(2009)
Garley, M., et al. Adv Med Sci 53(2):326-330(2008)

AM06000PU-N Interleukin-25 antibody staining of Formalin-Fixed Paraffin-Embedded Human Adrenal cortex at 10 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen.

AM06000PU-N

Western blot analysis of IL25 in mouse testis lysate. Using IL25 monoclonal antibody, clone 68C1039.2 ( Cat # MAB0090 ) . 10 µg of lysate was used per lane.

MAB0090

Western Blot analysis of IL25 expression in transfected 293T cell line (H00064806-T02) by IL25 MaxPab polyclonal antibody.Lane 1: IL17E transfected lysate(19.47 KDa).Lane 2: Non-transfected lysate.

H00064806-B01P

Immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue reacted with Interleukin-25 / IL25 Antibody (Center) Cat.-No AP52193PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

AP52193PU-N

Western blot analysis of Interleukin-25 / IL25 Antibody (Center) Cat.-No AP52193PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the IL25 antibody detected the IL25 protein (arrow).

AP52193PU-N

AP32729PU-N Interleukin-25 / IL25 Antibody staining of Human Prostate lysate at 0.3µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32729PU-N

AP32730PU-N Interleukin-25 / IL25 Antibody staining of Human Prostate lysate at 0.5µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

AP32730PU-N

TP320754

TP723202

Western Blot of Mouse anti-IL-17E antibody in full length recombinant protein using IL-17E antibody at 0.5 ug/ml.

TA319594

Flow Cytometry of Mouse anti-IL-17E antibody Cells: PC3 cells Primary antibody: 1 ug of IL-17E (red), control (green) Secondary antibody: anti-mouse IgG PE conjugated secondary antibody.

TA319594

Anti-IL-17E Biotin Conjugated Antibody - Western Blot Western Blot of Mouse anti-IL-17E antibody in full length recombinant protein using IL-17E antibody at 0.5 ug/mL

TA319595

Anti-IL-17E Antibody - Flow Cytometry Cells: PC3 cells Primary antibody: 1 ug of IL-17E (red), control (green) Secondary antibody: anti-mouse IgG PE conjugated secondary antibody

TA319595

To detect Human IL-17E by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17E (TA328241) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17E.

TA328240

To detect Human IL-17E by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17E (TA328240) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17E.

TA328241

To detect Human IL-17E by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-17E (TA328240) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL-17E.

TA328241

Western Blot: IL17E Antibody (68C1039.2) [TA336397] - Analysis of IL-17E using IL-17E monoclonal antibody. Mouse testis lysate probed with IL-17E antibody at 2 ug/ml.

TA336397

Immunohistochemistry: IL17E Antibody (68C1039.2) [TA336397] - Adrenal cortex, Human: Formalin-Fixed Paraffin-Embedded (FFPE)

TA336397

Western Blot: IL17E Antibody (8B1) [TA337154] - Analysis of IL17E in full-length recombinant protein using IL17E antibody at 0.5 ug/ml.

TA337154

Flow Cytometry: IL17E Antibody (8B1) [TA337154] - Intracellular analysis of IL-17E in PC3 cells using 1 ug of IL-17E antibody (green histogram represents isotype control antibody this antibody , red represents IL-17E antibody. this antibody was used for t

TA337154

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
AP32729PU-N	Interleukin-25 / IL25 (66-79) antibody
	Goat			Aff - Purified	Hu	WB	0.1 mg / €380.00
							OriGene Technologies GmbH
	OriGene recommends this product for Western blotting.
AP32730PU-N	Interleukin-25 / IL25 antibody
	Goat			Aff - Purified	Hu	WB	0.1 mg / €380.00
							OriGene Technologies GmbH
	OriGene recommends this product for Western blotting.
TA319594	Interleukin-25 / IL25 antibody
	Mouse monoclonal IL-17E antibody
	Mouse		11K8B1	Purified	Hu	F, WB	0.1 mg / €515.00
							OriGene Technologies, Inc.
+1 additional image
TA319595	Interleukin-25 / IL25 antibody
	Mouse monoclonal Anti-Human IL-17E Biotin Conjugated antibody
	Mouse		11K8B1	Purified	Hu	F, WB	0.1 mg / €515.00
							OriGene Technologies, Inc.
+1 additional image
TA328240	Interleukin-25 / IL25 antibody
	Biotinylated Anti-Human IL-17E Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu	E, WB	50 µg / €260.00
							OriGene Technologies, Inc.
TA328241	Interleukin-25 / IL25 antibody
	Anti-Human IL-17E Rabbit Polyclonal Antibody
	Rabbit	IgG		Purified	Hu	E, WB	0.1 mg / €275.00
							OriGene Technologies, Inc.
+1 additional image
TA336397	Interleukin-25 / IL25 antibody
	Mouse Monoclonal IL-17E/IL-25 Antibody (68C1039.2)
	Mouse	IgG1	68C1039.2	Purified	Hu, Ms	C, P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+1 additional image
TA337154	Interleukin-25 / IL25 antibody
	Mouse Monoclonal IL-17E/IL-25 Antibody (8B1)
	Mouse	IgG1	8B1	Purified	Hu	F, WB	0.1 mg / €395.00
							OriGene Technologies, Inc.
+1 additional image
AM06000PU-N	Interleukin-25 / IL25 (115-132) antibody
	Mouse	IgG1	68C1039.2	Purified	Hu, Ms	P, WB	50 µg / €425.00
							OriGene Technologies GmbH
AP52193PU-N	Interleukin-25 / IL25 (Center) antibody
	Rabbit	Ig		Aff - Purified	Hu	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image
MO15111-500	Interleukin-25 / IL25 (33-177) antibody
	Mouse	IgG1	-	Purified	Hu	E, F, WB	0.5 mg / €410.00
							Neuromics Antibodies

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP320754	Interleukin-25 / IL25 (transcript variant 2)
	Purified recombinant protein of Homo sapiens interleukin 25 (IL25), transcript variant 2
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

TP723202	Interleukin-25 / IL25 (transcript variant 2)
	Purified recombinant protein of Human interleukin 25 (IL25), transcript variant 2.
	Human	> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.	E. coli	25 µg / €230.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY406709	IL25 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY411546	IL25 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.
LY430366	IL25 overexpression lysate
		0.1 mg / €315.00
		OriGene Technologies, Inc.

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