Intrinsic factor antibody
Principal name
Intrinsic factor antibody
Alternative names for Intrinsic factor antibody
Gastric intrinsic factor, GIF, IFMH, INF
Ncbi ID
9606, NP_005133, NP_005133.2, NM_005142, NM_008118, NM_017162, NP_032144.2
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)
Background of Intrinsic factor antibody
- Kit Component:
- - KN207777G1, GIF gRNA vector 1 in pCas-Guide vector
- KN207777G2, GIF gRNA vector 2 in pCas-Guide vector
- KN207777D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)
Andersen, C.B., et al. Nature 464(7287):445-448(2010)
Ament, A.E., et al. Br. J. Haematol. 144(4):622-624(2009)
Remacha, A.F., et al. Ann. Hematol. 87(7):599-600(2008)
Mathews, F.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(44):17311-17316(2007)
Detection limit for recombinant GST tagged GIF is approximately 0.3ng/ml as a capture antibody.
Western Blot analysis of GIF expression in transfected 293T cell line (H00002694-T01) by GIF MaxPab polyclonal antibody.Lane 1: GIF transfected lysate(45.87 KDa).Lane 2: Non-transfected lysate.
GIF MaxPab rabbit polyclonal antibody. Western Blot analysis of GIF expression in mouse stomach.
Western Blot analysis of GIF expression in transfected 293T cell line (H00002694-T01) by GIF MaxPab polyclonal antibody.Lane 1: GIF transfected lysate(45.40 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with Intrinsic factor Antibody (Center) Cat.-No AP51837PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of Intrinsic factor Antibody (Center) Cat.-No AP51837PU-N with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of Intrinsic factor Antibody (Center) Cat.-No AP51837PU-N in K562 cell line lysates (35ug/lane). This demonstrates the GIF antibody detected the GIF protein (arrow).
Flow cytometric analysis of K562 cells using Intrinsic factor Antibody (Center) Cat.-No AP51837PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Host: Rabbit; Target Name: Gif; Sample Tissue: Mouse Stomach lysates; Antibody Dilution: 1.0 ug/ml
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP51837PU-N | Intrinsic factor (Center) antibody |
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 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
 +3 additional images |
Highly validated antibody! |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307777 | Intrinsic factor |
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| Recombinant protein of human gastric intrinsic factor (vitamin B synthesis) (GIF) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY417460 | GIF overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR215639L2V | Lenti ORF particles, Gif (GFP-tagged) - Mouse gastric intrinsic factor (Gif), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR215639L1V | Lenti ORF particles, Gif (Myc-DDK-tagged) - Mouse gastric intrinsic factor (Gif), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR210184L2V | Lenti ORF particles, Gif (GFP-tagged ORF) - Rat gastric intrinsic factor (Gif), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR210184L1V | Lenti ORF particles, Gif (Myc-DDK-tagged ORF) - Rat gastric intrinsic factor (Gif), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||