IPPK antibody
Principal name
IPPK antibody
Alternative names for IPPK antibody
IPK1 homolog, C9orf12, Inositol-pentakisphosphate 2-kinase, Inositol-1,3,4,5,6-pentakisphosphate 2-kinase, Ins(1,3,4,5,6)P5 2-kinase
Ncbi ID
9606, NP_073592, NP_073592.1, NM_199056, NM_022755, NP_951011.1, BC024630, NM_001008556
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of IPPK antibody
- Kit Component:
- - KN308392G1, Ippk gRNA vector 1 in pCas-Guide vector
- KN308392G2, Ippk gRNA vector 2 in pCas-Guide vector
- KN308392D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
Immunofluorescence of purified MaxPab antibody to IPPK on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of IPPK expression in transfected 293T cell line (H00064768-T01) by IPPK MaxPab polyclonal antibody.Lane 1: IPPK transfected lysate(54.01 KDa).Lane 2: Non-transfected lysate.
Immunofluorescence of purified MaxPab antibody to IPPK on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of IPPK expression in transfected 293T cell line (H00064768-T01) by IPPK MaxPab polyclonal antibody.Lane 1: IPPK transfected lysate(54.01 KDa).Lane 2: Non-transfected lysate.
Mouse Liver; WB Suggested Anti-Ippk Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Ippk antibody - C-terminal region (ARP57656_P050) in Mouse Liver cells using Western Blot
Immunohistochemical analysis of IPPK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of IPPK staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of IPPK expression in MCF7 (A); NIH3T3 (B) whole cell lysates.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IPPK antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HepG2 cells, using IPPK antibody.The picture on the right is treated with the synthesized peptide.
WB Suggested Anti-Ippk Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver
WB Suggested Anti-IPPK Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315745 | IPPK antibody |
||||||
| Rabbit polyclonal anti-IPPK antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, ICC/IF, P |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP305175 | IPPK |
||||
| Recombinant protein of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPPK) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY402937 | IPPK overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206804L2V | Lenti ORF particles, Ippk (GFP-tagged) - Mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase (cDNA clone MGC:28631 IMAGE:4222376), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR207832L2V | Lenti ORF particles, Ippk (GFP-tagged) - Mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,180.00
|
|
| OriGene Technologies, Inc. | ||
| MR206804L1V | Lenti ORF particles, Ippk (Myc-DDK-tagged) - Mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase (cDNA clone MGC:28631 IMAGE:4222376), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR207832L1V | Lenti ORF particles, Ippk (Myc-DDK-tagged) - Mouse inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR209419L2V | Lenti ORF particles, Ippk (GFP-tagged ORF) - Rat inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,170.00
|
|
| OriGene Technologies, Inc. | ||
| RR209419L1V | Lenti ORF particles, Ippk (Myc-DDK-tagged ORF) - Rat inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ippk), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,050.00
|
|
| OriGene Technologies, Inc. | ||