IQGAP1 antibody
Principal name
IQGAP1 antibody
Alternative names for IQGAP1 antibody
KIAA0051, p195, Ras GTPase-activating-like protein IQGAP1
Gene ID
8826 (IQGAP1)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)
Background of IQGAP1 antibody
- Kit Component:
- - KN218549G1, IQGAP1 gRNA vector 1 in pCas-Guide vector
- KN218549G2, IQGAP1 gRNA vector 2 in pCas-Guide vector
- KN218549D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ren JG, Li Z, Sacks DB,
IQGAP1 integrates Ca2+/calmodulin and B-Raf signaling.
The Journal of biological chemistry 2008 Aug 283 (34): 22972-82.
Immunoperoxidase of monoclonal antibody to IQGAP1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1 ug/ml]
Immunofluorescence of monoclonal antibody to IQGAP1 on HeLa cell. [antibody concentration 10 ug/ml]
IQGAP1 monoclonal antibody (M01), clone 2C5 Western Blot analysis of IQGAP1 expression in C32 ( Cat # L002V1 ).
Detection limit for recombinant GST tagged IQGAP1 is approximately 3ng/ml as a capture antibody.
Figure 1. Western blot analysis of IQGAP1 expression in HeLa cells (Lane 1), A431 cells (Lane 2), C2C12 cells (Lane 3), NIH/3T3 cells (Lane 4) and Rat-1 cells (Lane 5) using IQGAP1 antibody AM20036AF-N.
Figure 2. Immunoprecipitation of IQGAP1 from Hela cells with normal Mouse IgG (Lane 1) or IQGAP1 antibody AM20036AF-N (Lane 2). After immunoprecipitated with the antibody, immunocomplexwas resolved on SDS-PAGE and immunoblotted with IQGAP1 antibody AM20036AF-N.
Western blot analysis of IQGAP1 expression in Jurkat (A); HUVEC (B); F9 (C) whole cell lysates.
(1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA311278 | IQGAP1 antibody |
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| Goat Anti-IQGAP1 Antibody | |||||||
 |
Goat | Aff - Purified | Hu, Ms, Rt | E, WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AM20036AF-N | IQGAP1 antibody |
||||||
 |
Mouse | IgG2a | VIC1/IC11/IVB2 | Azide Free | Hu, Ms, Rt | IP, WB |
0.1 mg /
€385.00
|
| OriGene Technologies GmbH | |||||||
 +1 additional image |
|||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN218549 | IQGAP1 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN218549BN | IQGAP1 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN218549LP | IQGAP1 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN218549RB | IQGAP1 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||