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IRAK1 antibody

Principal name

IRAK1 antibody

Alternative names for IRAK1 antibody

IRAK-1, IRAK, Interleukin-1 receptor-associated kinase 1

SwissProt ID

P51617 (Human), Q2LGB3 (Bovin), Q62406 (Mouse)

Gene ID

3654 (IRAK1), 16179 (Irak1)

Ncbi ID

NP_001020413.1, 9606, NP_001020413, AAH14963.1, NP_001560.2, NM_001025242, NM_001025243, NP_001020414, P51617, NM_001177973, NM_001177974, NM_001177975, NM_001177976, NP_001171445.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of IRAK1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.The tyrosine-like kinase (TLK) group consists of 40 tyrosine and serine-threonine kinases such as MLK (mixed-lineage kinase), LISK (LIMK/TESK), IRAK (interleukin-1 receptor-associated kinase), Raf, RIPK (receptor-interacting protein kinase), and STRK (activin and TGF-beta receptors) families.

Immunocytochemical staining of HeLa cells with Rabbit anti Human IRAK-1 (SP1187P)

SP1187P

Western Blot analysis of THP-1 (THP) and HeLa (HL) cell lysates probed with Rabbit anti Human IRAK-1 (SP1187P)

SP1187P

Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

H00003654-M01

Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10 ug/ml]

H00003654-M01

IRAK1 monoclonal antibody (M01), clone 3F7 Western Blot analysis of IRAK1 expression in K-562 ( Cat # L009V1 ).

H00003654-M01

Detection limit for recombinant GST tagged IRAK1 is approximately 0.1ng/ml as a capture antibody.

H00003654-M01

Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]

H00003654-M02

Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10 ug/ml]

H00003654-M02

Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ( Cat # H00003654-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody (M02) clone 3A9 (Cat # H00003654-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

H00003654-M02

Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody (M02), clone 3A9.Lane 1: IRAK1 transfected lysate(68 KDa).Lane 2: Non-transfected lysate.

H00003654-M02

IRAK1 monoclonal antibody (M02), clone 3A9 Western Blot analysis of IRAK1 expression in A-431 ( Cat # L015V1 ).

H00003654-M02

Detection limit for recombinant GST tagged IRAK1 is approximately 0.03ng/ml as a capture antibody.

H00003654-M02

Western blot (WB) analysis of IRAK1 antibody (Cat.-No.: AP06527PU-N) in extracts from HeLa cells.

AP06527PU-N

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Brain, cortex.

AP07593PU-N

Western blot analysis of IRAK1. Using IRAK1 polyclonal antibody ( Cat # PAB0158 ) at 0.5 µg/ml against 15 µg/lane of HeLa and NIH/3T3 cell lysate.

PAB0158

Formalin-fixed and paraffin-embedded human cancer tissue reacted with IRAK1 polyclonal antibody ( Cat # PAB3469 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.

PAB3469

Western blot analysis of IRAK1 polyclonal antibody ( Cat # PAB3469 ) in Jurkat cell lysate. IRAK1 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

PAB3469

Western Blot analysis of IRAK1 expression in transfected 293T cell line (H00003654-T01) by IRAK1 MaxPab polyclonal antibody.Lane 1: IRAK1 transfected lysate(69.63 KDa).Lane 2: Non-transfected lysate.

H00003654-B01

Western Blot analysis of IRAK1 expression in transfected 293T cell line (H00003654-T01) by IRAK1 MaxPab polyclonal antibody.Lane 1: IRAK1 transfected lysate(68.00 KDa).Lane 2: Non-transfected lysate.

H00003654-D01P

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

AP14600PU-N

Western blot analysis of anti-IRAK1 Pab in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

AP14600PU-N

Western Blot analysis of IRAK1 expression in transfected 293T cell line (H00003654-T01) by IRAK1 MaxPab polyclonal antibody.Lane 1: IRAK1 transfected lysate(69.63 KDa).Lane 2: Non-transfected lysate.

H00003654-B01P

Immunocytochemistry using IRAK1 polyclonal antibody ( Cat # PAB10249 ) shows staining of IRAK1 in HeLa cells.The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1 : 100.

PAB10249

Western blot using IRAK1 polyclonal antibody ( Cat # PAB10249 ) shows detection of IRAK1 in THP-1 whole cell lysate ( THP ) or HeLa whole cell lysate ( HL ). The membrane was probed with the primary antibody diluted to 1 : 2,000.

PAB10249

Immunohistochemistry analyzes of IRAK1 antibody (Cat.-No.: AP20311PU-N) in paraffin-embedded human breast carcinoma tissue.

AP20311PU-N

Western blot analysis of IRAK1 antibody (Cat.-No.: AP20311PU-N) in extracts from NIH/3T3 cells.

AP20311PU-N

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using IRAK1 (Phospho-Thr100) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells treated with heat shock, using IRAK1 (Phospho-Thr100) Antibody. The lane on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, using IRAK1 (Phospho-Thr209) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using IRAK1 (Phospho-Ser376) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, using IRAK1 (Ab-387) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IRAK1 (Ab-209) Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from NIH-3T3 cells, using IRAK1 (Ab-209) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using IRAK1 (Ab-376) Antibody. The picture on the right is treated with the synthesized peptide.

Human Placenta (formalin-fixed, paraffin-embedded) stained with IRAK1 antibody AP22789PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

AP22789PU-N

(LEFT) Western blot analysis of IRAK1 in Jurkat cell lysate. IRAK1 (RIGHT) Western blot analysis of IRAK1 in 293 cell lysates (2 µg/lane) either non-transfected (Lane 1) or transiently transfected with the IRAK1 gene (Lane 2).

AP22789PU-N

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM20904PU-N

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM20904PU-N

Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell (antibody concentration 10 µg/ml).

AM20904PU-N

IRAK1 monoclonal antibody clone 3F7 Western Blot analysis of IRAK1 expression in K-562.

AM20904PU-N

Detection limit for recombinant GST tagged IRAK1 is approximately 0.1 ng/ml as a capture antibody.

AM20904PU-N

Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

AM20905PU-N

Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell (antibody concentration 10 µg/ml).

AM20905PU-N

WB analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 MAb clone 3A9. GAPDH ( 36.1 kD ) used as specificity and loading ctrl.

AM20905PU-N

IRAK1 monoclonal antibody clone 3A9 Western Blot analysis of IRAK1 expression in A-431.

AM20905PU-N

Western Blot analysis of IRAK1 expression in transfected 293T cell line by IRAK1 monoclonal antibody clone 3A9.

AM20905PU-N

Detection limit for recombinant GST tagged IRAK1 is approximately 0.03 ng/ml as a capture antibody.

AM20905PU-N

Immunohistochemical analysis of paraffin-embedded human brain tissue using IRAK1 (Phospho-Ser376) antibody AP55759PU-N (left)or the same antibody preincubated with blocking peptide (right).

Western blot analysis of IRAK1 (pT209) expression in MCF7 IL1b-treated (A); K562 (B); MCF7 (C); Raw264.7 IL1b-treated (D) whole cell lysates.

TP304869

Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with IRAK antibody at 1:2000

TA305934

Immunohistochemistry of IRAK in HeLa cells with IRAK antibody at 10 ug/ml.

TA305934

Immunocytochemistry of IRAK in HeLa cells with IRAK antibody at 10 ug/mL.

TA305934

Immunofluorescence of IRAK in HeLa cells with IRAK antibody at 20 ug/mL.

TA305934

Immunohistochemistry analysis of paraffin-embedded human brain tissue using IRAK1 antibody.The picture on the right is treated with the synthesized peptide.

TA314086

Western blot analysis of IRAK in HeLa lysates with IRAK antibody at (A) 1 and (B) 2 ug/mL.

TA319667

Immunohistochemistry of IRAK in rat liver tissue with IRAK antibody at 2.5 ug/mL.

TA319667

Western blot analysis of IRAK in mouse liver lysate with IRAK antibody at (A) 1 and (B) 2 ug/mL.

TA319668

Immunohistochemistry of IRAK in rat liver tissue with IRAK antibody at 5 ug/mL.

TA319668

Western Blot: IRAK Antibody [TA336450] - Analysis of IRAK-1 in 1) human HeLa and 2) mouse NIH3T3 lysate using IRAK-1 antibody at 1 ug/ml.

TA336450

Western blot analysis of extracts from HeLa cells, using IRAK1 (Phospho-Thr209) antibody.The lane on the right is treated with the synthesized peptide.

TA314091

Western blot analysis of extracts from Jurkat cells, treated with heat shock, using IRAK1 (Phospho-Thr100) antibody.The lane on the right is treated with the synthesized peptide.

TA314090

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRAK1 (Phospho-Thr100) antibody.The picture on the right is treated with the synthesized peptide.

TA314090

Immunohistochemistry analysis of paraffin-embedded human brain tissue using IRAK1 (Phospho-Ser376) antibody.The picture on the right is treated with the synthesized peptide.

TA314089

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA305934	IRAK1 (C-term) antibody
	Rabbit Polyclonal IRAK Antibody
	Rabbit	IgG		Purified	Hu, Ms, Rt	E, ICC/IF, IP, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+3 additional images	Highly validated antibody!
AM20904PU-N	IRAK1 antibody
	Mouse	IgG2a	3F7		Hu	E, ICC/IF, P, WB	50 µg / €475.00
							OriGene Technologies GmbH
+4 additional images	Highly validated antibody!
AM20905PU-N	IRAK1 antibody
	Mouse	IgG1	3A9		Hu	E, ICC/IF, IP, P, RNAi, WB	50 µg / €475.00
							OriGene Technologies GmbH
+5 additional images	Highly validated antibody!
TA314086	IRAK1 antibody
	Rabbit polyclonal anti-IRAK1 antibody
	Rabbit	IgG		Purified	Hu	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA314089	IRAK1 antibody
	Rabbit polyclonal IRAK1 (Ser376) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu	E, P	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA314090	IRAK1 antibody
	Rabbit polyclonal IRAK1 (Thr100) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu, Ms	E, P, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
+1 additional image
TA314091	IRAK1 antibody
	Rabbit polyclonal IRAK1 (Thr209) antibody(Phospho-specific)
	Rabbit	IgG		Purified	Hu	E, WB	0.1 ml / €335.00
							OriGene Technologies, Inc.
TA319668	IRAK1 (618-712) antibody
	Mouse IRAK Monoclonal Antibody
	Mouse	IgG	8F1A7	Aff - Purified	Hu, Ms	E, P, WB	0.1 mg / €415.00
							OriGene Technologies, Inc.
+1 additional image
TA336450	IRAK1 antibody
	Rabbit Polyclonal IRAK1 Antibody
	Rabbit			Purified	Hu, Ms, Rt	IP, WB	0.1 mg / €335.00
							OriGene Technologies, Inc.
AP06527PU-N	IRAK1 antibody
	Rabbit			Aff - Purified	Hu, Ms	ICC/IF, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
AP07593PU-N	IRAK1 antibody
	Rabbit			Purified	Hu, Ms, Rt	E, IP, P, WB	50 µg / €425.00
							OriGene Technologies GmbH
AP14600PU-N	IRAK1 (C-term) antibody
	Rabbit	Ig		Purified	Hu	P, WB	0.4 ml / €415.00
							OriGene Technologies GmbH
+1 additional image
AP20311PU-N	IRAK1 antibody
	Rabbit			Aff - Purified	Hu	P, WB	0.1 mg / €370.00
							OriGene Technologies GmbH
+1 additional image
AP22789PU-N	IRAK1 (C-term) antibody
	Rabbit			Purified	Hu	P, WB	50 µg / €425.00
							OriGene Technologies GmbH
+1 additional image
AP55759PU-N	IRAK1 pSer376 antibody
	Rabbit			Aff - Purified	Hu	P	0.1 mg / €300.00
							OriGene Technologies GmbH
AP55759PU-S	IRAK1 pSer376 antibody
	Rabbit			Aff - Purified	Hu	P	50 µg / €230.00
							OriGene Technologies GmbH
SP1187P	IRAK1 antibody
	Rabbit			Purified	Hu	ICC/IF, IP, WB	0.1 mg / €410.00
							OriGene Technologies GmbH
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP304869	IRAK1 (transcript variant 3)
	Recombinant protein of human interleukin-1 receptor-associated kinase 1 (IRAK1), transcript variant 3
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
				OriGene Technologies, Inc.

Lysates

Catalog No.
LY422494	IRAK1 overexpression lysate
		0.1 mg / €495.00
		OriGene Technologies, Inc.
LY422495	IRAK1 overexpression lysate
		0.1 mg / €295.00
		OriGene Technologies, Inc.

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