IRAK2 antibody
Principal name
IRAK2 antibody
Alternative names for IRAK2 antibody
IRAK-2, Interleukin-1 receptor-associated kinase-like 2
Ncbi ID
NP_001561.3, 9606, 10090, NP_001107025.1, NP_001561, NM_001570, AAO24761, NM_001113553, NM_172161, NM_001025422, BC085324
Available reactivities
Available hosts
Available applications
Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of IRAK2 antibody
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
Immunocytochemical staining of HeLa cells with Rabbit anti IRAK2 (SP1272P)
Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with SP1272P.
Immunoperoxidase of monoclonal antibody to IRAK2 on formalin-fixed paraffin-embedded human transitional cell carcinoma. [antibody concentration 3 ug/ml]
IRAK2 monoclonal antibody (M01), clone 6C7 Western Blot analysis of IRAK2 expression in K-562 ( Cat # L009V1 ).
IRAK2 monoclonal antibody (M02), clone 3G6 Western Blot analysis of IRAK2 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged IRAK2 is approximately 0.3ng/ml as a capture antibody.
IRAK2 monoclonal antibody (M03), clone 3H1 Western Blot analysis of IRAK2 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
IRAK2 monoclonal antibody (M04), clone 1A6. Western Blot analysis of IRAK2 expression in NIH/3T3 ( Cat # L018V1 ).
IRAK2 monoclonal antibody (M04), clone 1A6. Western Blot analysis of IRAK2 expression in PC-12 ( Cat # L012V1 ).
IRAK2 monoclonal antibody (M04), clone 1A6 Western Blot analysis of IRAK2 expression in HeLa ( Cat # L013V1 ).
Detection limit for recombinant GST tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
IRAK2 monoclonal antibody (M05), clone 4B6 Western Blot analysis of IRAK2 expression in HeLa ( Cat # L013V1 ).
IRAK2 monoclonal antibody (M05), clone 4B6. Western Blot analysis of IRAK2 expression in NIH/3T3 ( Cat # L018V1 ).
Detection limit for recombinant GST tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
IRAK2 monoclonal antibody (M06), clone 4C11 Western Blot analysis of IRAK2 expression in NIH/3T3 ( Cat # L018V1 ).
Detection limit for recombinant GST tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
Western blot analysis of IRAK2 polyclonal antibody ( Cat # PAB3470 ) in Jurkat cell lysate. IRAK2 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with IRAK2 polyclonal antibody ( Cat # PAB3471 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of IRAK2 polyclonal antibody ( Cat # PAB3471 ) in K-562 cell lysate. IRAK2 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
IRAK2 monoclonal antibody (M01), clone 6C7 Western Blot analysis of IRAK2 expression in K-562 ( Cat # L009V1 ).
IRAK2 monoclonal antibody (M02), clone 3G6 Western Blot analysis of IRAK2 expression in HeLa ( Cat # L013V1 ).
Western blot analysis of anti-IRAK2 Pab in Jurkat cell lysate. IRAK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-IRAK2 Pab in K562 cell lysate. IRAK2 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Skin: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunocytochemistry using IRAK2 polyclonal antibody ( Cat # PAB10250 ) shows staining of IRAK2 in HeLa cells.The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1 : 500.
Western blot using IRAK2 polyclonal antibody ( Cat # PAB10250 ) shows detection of IRAK2 in HeLa ( H ) and K-562 ( K ) whole cell lysates. The membrane was probed with the primary antibody diluted to 1 : 500.
Immunofluorescence of IRAK2 in HeLa cells with IRAK2 polyclonal antibody ( Cat # PAB13083 ) at 10 µg/ml.
Western blot analysis of IRAK2 in K-562 whole cell lysate with IRAK2 polyclonal antibody ( Cat # PAB13083 ) at 1 : 500 dilution.
Immunocytochemistry of Irak2 in A-20 cells with Irak2 polyclonal antibody ( Cat # PAB13161 ) at 1 µg/ml.
Western blot analysis of Irak2 in RAW264.7 whole cell lysate with Irak2 polyclonal antibody ( Cat # PAB13161 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Western blot analysis of Irak2 in A-20 whole cell lysate with Irak2 polyclonal antibody ( Cat # PAB13162 ) at ( A ) 0.5, ( B ) 1, and ( C ) 2 µg/ml.
Human Liver (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22850PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Spleen (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22850PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22850PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunocytochemical staining of HeLa cells using IRAK2 antibody (AP22850PU-N) at 2 µg/ml.
Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (AP22850PU-N) at 2 µg/ml.
Human Testis (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22862PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Liver (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22862PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Placenta (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22862PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Pancreas (formalin-fixed, paraffin-embedded) stained with IRAK2 antibody AP22862PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunocytochemistry of mIRAK2 in A-20 cells with mIRAK2 antibody (AP22862PU-N) at 1 µg/ml.
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody (AP22862PU-N) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Human Fetal liver; WB Suggested Anti-IRAK2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Liver; IRAK2 antibody - C-terminal region (ARP61222_P050) in Human Fetal liver cells using Western Blot
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot analysis of IRAK2 expression in K-562 cell lysate.
Western blot analysis of IRAK2 expression in HeLa (A); K562 (B); MCF7 (C); Ramos (D) whole cell lysates.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IRAK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA803648)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRAK2 (RC218437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRAK2.
E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 362-625 of human IRAK2 (NP_001561) were separated by SDS-PAGE and immunoblotted with anti-IRAK2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRAK2 (RC218437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRAK2.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRAK2 (RC218437, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRAK2.
Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 ug/mL.
Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 ug/mL.
Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 ug/mL.
Western blot analysis of IRAK2 in K562 whole cell lysate with IRAK2 antibody at 1:500 dilution.
Immunofluorescence of IRAK2 in HeLa cells with IRAK antibody at 10 ug/mL.
Western blot analysis of IRAK2 in RAW264.7 whole cell lysate with mIRAK2 antibody at (A) 0.5, (B) 1, and (C) 2 ug/mL.
Immunocytochemistry of IRAK2 in A-20 cells with IRAK2 antibody at 1 ug/mL.
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 10 ug/mL.
Western blot analysis of IRAK2 in A-20 whole cell lysate with IRAK2 antibody (C2) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Western blot analysis of extracts from 293 cells, using IRAK2 antibody.The lane on the right is treated with the synthesized peptide.
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP22850PU-N | IRAK2 (C-term) antibody |
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Rabbit | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
 +4 additional images |
Highly validated antibody! |
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| AP22862PU-N | IRAK2 (C-term) antibody |
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 |
Rabbit | Purified | Hu | E, ICC/IF, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +5 additional images |
Highly validated antibody! |
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| TA305997 | IRAK2 antibody |
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| Rabbit Polyclonal IRAK-2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA306017 | IRAK2 (C-term) antibody |
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| Rabbit Polyclonal IRAK-2 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA306247 | IRAK2 (C-term) antibody |
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| Rabbit Polyclonal IRAK2 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | E, ICC/IF, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|||||||
| TA306248 | IRAK2 antibody |
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| Rabbit Polyclonal IRAK2 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Ms | E, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA314092 | IRAK2 antibody |
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| Rabbit polyclonal anti-IRAK2 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA803648 | IRAK2 antibody |
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| IRAK2 mouse monoclonal antibody, clone OTI4C1 (formerly 4C1) | |||||||
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Mouse | IgG1 | OTI4C1 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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| TA803676 | IRAK2 antibody |
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| Purified IRAK2 mouse monoclonal antibody, clone OTI1B4 (formerly 1B4) | |||||||
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Mouse | IgG1 | OTI1B4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803677 | IRAK2 antibody |
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| IRAK2 mouse monoclonal antibody, clone OTI2B7 (formerly 2B7) | |||||||
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Mouse | IgG1 | OTI2B7 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA803679 | IRAK2 antibody |
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| IRAK2 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4) | |||||||
 |
Mouse | IgG1 | OTI1D4 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| AM31802PU-N | IRAK2 antibody |
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 |
Mouse | IgG1 | 6C7 | Purified | Hu | E, P, WB |
50 µg /
€475.00
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP08453PU-N | IRAK2 antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
50 µg /
€425.00
|
|
| OriGene Technologies GmbH | |||||||
| AP14601PU-N | IRAK2 (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP14602PU-N | IRAK2 (C-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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