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IREB2 antibody

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Principal name

IREB2 antibody

Alternative names for IREB2 antibody

IRE-BP2, Iron regulatory protein 2, IRP2, Iron-responsive element-binding protein 2

SwissProt ID

P48200 (Human), Q62751 (Rat), Q811J3 (Mouse)

Gene ID

3658 (IREB2), 64831 (Ireb2)

Ncbi ID

NP_004127.1, NP_004127, NM_022863, NM_004136

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), African clawed frog, Prim (Primate), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of IREB2 antibody

Iron regulatory protein 2 (IREB2 or IRP2) is a central regulator of cellular iron homeostasis due to its regulation of specific mRNAs encoding proteins of iron uptake and storage. In mammalian cells, regulation of the expression of proteins involved in iron metabolism is achieved through interactions of iron-sensing proteins known as iron regulatory proteins (IRPs), with transcripts that contain RNA stem-loop structures referred to as iron responsive elements (IREs). Two distinct but highly homologous proteins, IRP1 and IRP2, bind IREs with high affinity when cells are depleted of iron, inhibiting translation of some transcripts, such as ferritin, or turnover of others, such as the transferrin receptor (TFRC). IRPs sense cytosolic iron levels and modify expression of proteins involved in iron uptake, export and sequestration according to the needs of individual cells. Regulating the amount of iron sequestered by ferritin and taken up by transferrin receptor ensures that cells acquire sufficient iron for their needs, while preventing iron toxicity.

General readings

Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010)
Hansen, H.M., et al. Hum. Mol. Genet. 19(18):3652-3661(2010)
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Salahudeen, A.A., et al. Science 326(5953):722-726(2009)
DeMeo, D.L., et al. Am. J. Hum. Genet. 85(4):493-502(2009)

H00003658-B02 - IREB2
Western Blot analysis of IREB2 expression in transfected 293T cell line (H00003658-T02) by IREB2 MaxPab polyclonal antibody.Lane 1: IREB2 transfected lysate(37.84 KDa).Lane 2: Non-transfected lysate.
H00003658-B01P - IREB2
Western Blot analysis of IREB2 expression in transfected 293T cell line (H00003658-T01) by IREB2 MaxPab polyclonal antibody.Lane 1: IREB2 transfected lysate(37.73 KDa).Lane 2: Non-transfected lysate.
PAB12137 - IREB2
Western blot analysis of Ireb2 in mouse liver lysate using Ireb2 polyclonal antibody ( Cat # PAB12137 ).
PAB12137 - IREB2
Western blot analysis of Ireb2 in RAW 264.7 lysate using Ireb2 polyclonal antibody ( Cat # PAB12137 ). Lane 1 : Lot B. Lane 2 : Lot C.
PAB12533 - IREB2
Western blot analysis of IREB2 in mouse liver lysate using IREB2 polyclonal antibody ( Cat # PAB12533 ).
ARP40509_P050 - IREB2
Mouse Liver; Researcher: Dr. Hao Zhu, University of Kansas Medical Center. Application: Western blotting. Species+tissue/cell type: Mouse liver extract. How many ug'sof tissue/cell lysate run on the gel:1. 60 ug mouse liver extract2. 60 ug mouse liver extract3. 60 ug mouse liver extract. Primary antibody dilution: 1:500. Secondary antibody: Anti-rabbit HRP. Secondary antibody dilution: 1:3000; IREB2 antibody - middle region (ARP40509_P050) in Mouse Liver cells using Western Blot
ARP40509_P050 - IREB2
Human Liver; WB Suggested Anti-IREB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Liver; IREB2 antibody - middle region (ARP40509_P050) in Human Liver cells using Western Blot
AP52232PU-N - IREB2
Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with IREB2 Antibody (Center) Cat.-No AP52232PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
AP52232PU-N - IREB2
Western blot analysis of IREB2 Antibody (Center) Cat.-No AP52232PU-N in Hela cell line lysates (35ug/lane). This demonstrates the IREB2 antibody detected the IREB2 protein (arrow).
AP52232PU-N - IREB2
Flow cytometric analysis of Hela cells using IREB2 Antibody (Center) Cat.-No AP52232PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TP321385 - IREB2
TA305765 - IREB2
TA305765 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA309703 - IREB2
Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western blotting using IRP2 or b-actin antibodies diluted 1:500 in TBS-Tween.
TA309703 - IREB2
Detection of IRP2 in mouse liver lysate using anti-IRP2.
TA345869 - IREB2
WB Suggested Anti-IREB2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Liver
TA345869 - IREB2
Application: Western blotting; Species+tissue/cell type: Mouse liver extract; How many ug'sof tissue/cell lysate run on the gel: 1. 60 ug mouse liver extract2. 60 ug mouse liver extract3. 60 ug mouse liver extract; Primary antibody dilution: 1: 500; Secondary antibody: Anti-rabbit HRP; Secondary antibody dilution: 1: 3000

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IREB2 antibody
  Goat Anti-IREB2 / IRP2 Antibody  
TA305765 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

IREB2 antibody
  Rabbit Polyclonal IRP2 Antibody  
Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western blotting using IRP2 or b-actin antibodies diluted 1:500 in TBS-Tween. Rabbit Purified Hu, Ms, Prim, Rt C
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

IREB2 (Center) antibody
Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with IREB2 Antibody (Center) Cat.-No AP52232PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+2 additional images

CRISPR

Catalog No. Species Donor Vector  

IREB2 - human gene knockout kit via CRISPR
IREB2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

IREB2 - human gene knockout kit via CRISPR
IREB2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

IREB2 - human gene knockout kit via CRISPR
IREB2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

IREB2
  Recombinant protein of human iron-responsive element binding protein 2 (IREB2)  
IREB2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ireb2 (GFP-tagged ORF) - Rat iron responsive element binding protein 2 (Ireb2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Ireb2 (GFP-tagged ORF) - Rat iron responsive element binding protein 2 (Ireb2), 200 uL, >10^7 TU/mL  
200 µl / €1,840.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Ireb2 (Myc-DDK-tagged ORF) - Rat iron responsive element binding protein 2 (Ireb2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Ireb2 (Myc-DDK-tagged ORF) - Rat iron responsive element binding protein 2 (Ireb2), 200 uL, >10^7 TU/mL  
200 µl / €1,650.00
  OriGene Technologies, Inc.
 

9 Item(s)

per page

Available Filters

Product Type
  1. CRISPR (3)
  2. Primary Antibody (3)
  3. Protein | Growth Factor (1)
  4. Rat Lenti ORF Particle (2)
Clonality
  1. Polyclonal (3)
Target
  1. IREB2 (4)
Property
  1. Center (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
  4. Primate (Prim) (1)
Applications
  1. Paraffin Sections (P) (2)
  2. Western blot / Immunoblot (WB) (2)
  3. Frozen sections (C) (1)
  4. Enzyme Immunoassay (E) (1)
  5. Flow cytometry (F) (1)
Presentation
  1. Aff - Purified (2)
  2. Purified (1)
Host
  1. Rabbit (2)
  2. Goat (1)
Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. Flow cytometry (F) (1)
  3. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Species (CRISPR)
  1. Human (3)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (9)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • fax: +49-5221-34606-11
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