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IRF5 antibody

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Principal name

IRF5 antibody

Alternative names for IRF5 antibody

Interferon regulatory factor 5, IRF-5

SwissProt ID

P56477 (Mouse), Q13568 (Human), Q58DJ0 (Bovin)

Gene ID

3663 (IRF5), 27056 (Irf5)

Ncbi ID

NP_001092097.2, 9606, NP_116032, NP_036187, NP_116032.1, NP_002191, NM_032643, NM_001098627, NP_001092099, NP_001092097, NP_001092100, NP_002191.1, NP_036187.1, NM_012057, NM_002200, NM_001098631, NM_001098628

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chk (Chicken), Por (Porcine), Ze (Zebrafish), African clawed frog, Eq (Equine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), ChIP assay (ChIP)

Background of IRF5 antibody

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3?/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-termil D-binding domains. IRF family members regulate transcription through interactions with proteins that share similar D-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E). IRF-5 is expressed in lymphoid tissues and peripheral blood lymphocytes and participates in the induction of type I interferon genes following viral infection. Activation of IRF-5 sigling is triggered by the toll-like receptor (TLR) pathway, including TLR7 and MyD88. Genetic variants of IRF-5 have been associated with disorders where the IFN pathway is abnormally activated, such as systemic lupus erythematosus .

General readings

Barnes B.J., et al (2001). J. Biol. Chem. 276:23382-23390
Schoenemeyer A., et al (2005). J. Biol. Chem. 280:17005-17012

SM6008 - IRF5
ImmunohistochemistryParaffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
SM6008 - IRF5
ICC/IF analysis of IRF5 in THP - 1 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti human THP - 1 antibody (1: 100) with goat anti mouse IgG - Alexa fluor 488 conjugate (Green).
SM6008 - IRF5
Flow cytometry analysis of IRF5 in THP - 1 cell line, staining at 2 - 5ug for 1x106 cells ( red line). The secondary antibody used goat anti mouse IgG Alexa fluor 488 conjugate . Isotype control antibody was mouse IgG (black line).
SM6008 - IRF5
Western blot analysis: The extracts of Ramos, THP-1, A20 and NIH3T3 were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IRF5 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
H00003663-M03 - IRF5
Immunofluorescence of monoclonal antibody to IRF5 on A-431 cell. [antibody concentration 10 ug/ml]
H00003663-M03 - IRF5
IRF5 monoclonal antibody (M03), clone 1H6. Western Blot analysis of IRF5 expression in NIH/3T3(Cat # L018V1 ).
H00003663-M03 - IRF5
IRF5 monoclonal antibody (M03), clone 1H6 Western Blot analysis of IRF5 expression in A-431 ( Cat # L015V1 ).
H00003663-M03 - IRF5
Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.
H00003663-M04 - IRF5
IRF5 monoclonal antibody (M04), clone 3C2 Western Blot analysis of IRF5 expression in A-431 ( Cat # L015V1 ).
H00003663-M05 - IRF5
Immunoperoxidase of monoclonal antibody to IRF5 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.8 ug/ml]
H00003663-M05 - IRF5
Immunofluorescence of monoclonal antibody to IRF5 on A-431 cell. [antibody concentration 10 ug/ml]
H00003663-M05 - IRF5
IRF5 monoclonal antibody (M05), clone 2D4 Western Blot analysis of IRF5 expression in A-431 ( Cat # L015V1 ).
ARP38131_T100 - IRF5
Human HepG2; WB Suggested Anti-IRF5 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; IRF5 antibody - N-terminal region (ARP38131_T100) in Human HepG2 cells using Western Blot
ARP37180_P050 - IRF5
Human Lymphnode; WB Suggested Anti-IRF5 Antibody Titration: 0.15ug/ml. ELISA Titer: 1:62500. Positive Control: Human Lymphnode; IRF5 antibody - N-terminal region (ARP37180_P050) in Human Lymphnode cells using Western Blot
PAB6227 - IRF5
IRF5 polyclonal antibody ( Cat # PAB6227 ) staining ( 2 µg/ml ) of A-549 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
MAB1068 - IRF5
Western blot analysis of extracts of Ramos , THP - 1 , A20 and NIH/3T3 were resolved by SDS - PAGE , transferred to PVDF membrane and probed with IRF5 monoclonal antibody , clone 10T1 ( 1 : 1000 ) ( Cat # MAB1068 ) . Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
H00003663-B01 - IRF5
Western Blot analysis of IRF5 expression in transfected 293T cell line (H00003663-T01) by IRF5 MaxPab polyclonal antibody.Lane 1: IRF5 transfected lysate(54.78 KDa).Lane 2: Non-transfected lysate.
H00003663-D01P - IRF5
Western Blot analysis of IRF5 expression in transfected 293T cell line (H00003663-T01) by IRF5 MaxPab polyclonal antibody.Lane 1: IRF5 transfected lysate(56.00 KDa).Lane 2: Non-transfected lysate.
ARP31994_P050 - IRF5
Human SH-SYSY; WB Suggested Anti-IRF5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: SH-SYSY cell lysate; IRF5 antibody - middle region (ARP31994_P050) in Human SH-SYSY cells using Western Blot
AR51437PU-N - IRF5
AM33091PU-S - IRF5
Western Blot analysis: THP-1 cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IRF5 Antibody Cat.-No AP33091PU (Clone 11F4A09). Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
TA507246 - IRF5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5.
TA507251 - IRF5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5.
TA507252 - IRF5
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252)
TA507252 - IRF5
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252)
TA507252 - IRF5
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252)
TA507252 - IRF5
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252)
TA507252 - IRF5
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252)
TA507252 - IRF5
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252)
TA507252 - IRF5
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5.
TP300458 - IRF5
TP311941 - IRF5
TP315434 - IRF5
TP315781 - IRF5
TA302667 - IRF5
TA302667 (0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA302667 - IRF5
HEK293 overexpressing human IRF5 and probed with TA302667 at 1ug/ml (mock transfection in lane B).
TA312432 - IRF5
Western blot analysis of extracts from HepG2 cells, using IRF5 Antibody.The lane on the right is treated with the synthesized peptide.
TA324738 - IRF5
Western blot analysis of anti-IRF5 Antibody (N-term) (Cat.#TA324738) in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab.
TA324738 - IRF5
Confocal immunofluorescent analysis of IRF5 Antibody (N-term) (Cat. #TA324738) with A549 cell followed by Alexa Fluor搴?489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
TA324738 - IRF5
IRF5 Antibody (N-term) (Cat. #TA324738) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA326952 - IRF5
Western blot analysis of extracts of various cell lines, using IRF5 antibody.
TA326952 - IRF5
Immunofluorescence analysis of HeLa cell using IRF5 antibody. Blue: DAPI for nuclear staining.
TA329736 - IRF5
WB Suggested Anti-IRF5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: SH-SYSY cell lysate
TA335428 - IRF5
WB Suggested Anti-IRF5 Antibody Titration: 2.5 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate
TA342305 - IRF5
WB Suggested Anti-IRF5 Antibody Titration: 0.15 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Lymphnode
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

IRF5 antibody
  IRF5 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)  
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507252) Mouse IgG1 OTI1G7 Purified Hu P, WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
+6 additional images

Highly validated antibody!

TrueMAB

IRF5 (176-240) antibody
Immunohistochemistry Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG1 10T1 Purified Hu, Ms E, F, ICC/IF, IP, P, WB
0.1 ml / €330.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

IRF5 (176-240) antibody
Immunohistochemistry Paraffin embedded sections of normal colon mucosa and colon adenocalcinoma tissue were incubated with anti-human IRF-5 antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Mouse IgG1 10T1 Purified Hu, Ms E, F, ICC/IF, IP, P, WB
50 µl / €220.00
  OriGene Technologies GmbH
+3 additional images

Highly validated antibody!

IRF5 (C-term) antibody
  Goat Polyclonal Antibody against IRF5  
TA302667 (0.1ug/ml) staining of lysate of cell line A549 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt ChIP, E, IP, P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.
+1 additional image

IRF5 antibody
  Rabbit polyclonal anti-IRF5 antibody  
Western blot analysis of extracts from HepG2 cells, using IRF5 Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

IRF5 (N-term) antibody
  Rabbit polyclonal IRF5 Antibody (N-term)  
Western blot analysis of anti-IRF5 Antibody (N-term) (Cat.#TA324738) in Ramos cell line lysates (35ug/lane). IRF5 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+2 additional images

IRF5 antibody
  IRF5 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using IRF5 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €335.00
  OriGene Technologies, Inc.
+1 additional image

IRF5 antibody
  IRF5 mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5. Mouse IgG2a OTI1A5 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

IRF5 antibody
  IRF5 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5. Mouse IgG1 OTI1B8 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

CRISPR

Catalog No. Species Donor Vector  

IRF5 - human gene knockout kit via CRISPR
IRF5 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

IRF5 - human gene knockout kit via CRISPR
IRF5 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

IRF5 - human gene knockout kit via CRISPR
IRF5 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

IRF5 - human gene knockout kit via CRISPR
IRF5 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Irf5 - mouse gene knockout kit via CRISPR
Irf5 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Irf5 - mouse gene knockout kit via CRISPR
Irf5 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Irf5 - mouse gene knockout kit via CRISPR
Irf5 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Irf5 - mouse gene knockout kit via CRISPR
Irf5 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

IRF5 (transcript variant 2)
  Recombinant protein of human interferon regulatory factor 5 (IRF5), transcript variant 2  
IRF5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

IRF5 (transcript variant 5)
  Recombinant protein of human interferon regulatory factor 5 (IRF5), transcript variant 5  
IRF5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

IRF5 (transcript variant 3)
  Recombinant protein of human interferon regulatory factor 5 (IRF5), transcript variant 3  
IRF5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

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24 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. CRISPR (8)
  2. Lysate (3)
  3. Primary Antibody (9)
  4. Protein | Growth Factor (4)
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Target
  1. IRF5 (13)
Property
  1. 176-240 (2)
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  3. N-term (1)
Reactivity
  1. Human (Hu) (9)
  2. Mouse (Ms) (5)
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Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Enzyme Immunoassay (E) (4)
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Images: Applications
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  3. Flow cytometry (F) (2)
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  5. Immunofluorescence (IF) (2)
Species (Protein)
  1. Human (4)
Source
  1. HEK293 cells (4)
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  1. GFP-Puro (2)
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Manufacturer
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Service
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