IRF6 antibody
Principal name
IRF6 antibody
Alternative names for IRF6 antibody
Interferon regulatory factor 6, IRF-6
Gene ID
3664 (IRF6)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of IRF6 antibody
IRF6 is a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-termil helix-turn-helix D-binding domain and a less conserved C-termil protein-binding domain. Mutations in its gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation.The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of aphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-termil helix-turn-helix D-binding domain and a less conserved C-termil protein-binding domain. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.
General readings
Kondo S, Schutte BC, Richardson RJ, Bjork BC, Knight AS, Watanabe Y, Howard E, de Lima RL, Daack-Hirsch S, Sander A, McDonald-McGinn DM, Zackai EH, Lammer EJ, Aylsworth AS, Ardinger HH, Lidral AC, Pober BR, Moreno L, Arcos-Burgos M, Valencia C, Houdayer C, Bahuau M, Moretti-Ferreira D, Richieri-Costa A, Dixon MJ, Murray JC.
Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes.
Nat Genet. 2002 Oct;32(2):285-9. Epub 2002 Sep 03.
PMID: 12219090
Western Blot analysis of IRF6 expression in transfected 293T cell line (H00003664-T02) by IRF6 MaxPab polyclonal antibody.Lane 1: IRF6 transfected lysate(51.37 KDa).Lane 2: Non-transfected lysate.
IRF6 polyclonal antibody ( Cat # PAB6150 ) staining ( 0.2 µg/ml ) of human ovary lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
IRF6 MaxPab polyclonal antibody. Western Blot analysis of IRF6 expression in human colon.
Western Blot analysis of IRF6 expression in transfected 293T cell line (H00003664-T01) by IRF6 MaxPab polyclonal antibody.Lane 1: IRF6 transfected lysate(51.48 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-IRF6 Antibody Titration: 2.5ug/ml. Positive Control: Jurkat cell lysate; IRF6 antibody - C-terminal region (ARP43608_T100) in Human Jurkat cells using Western Blot
Human Placenta; Immunohistochemistry with Placenta tissue at an antibody concentration of 5ug/ml using anti-IRF6 antibody (ARP31995_P050); IRF6 antibody - middle region (ARP31995_P050) in Human Placenta cells using Immunohistochemistry
r-IRF6; WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Transfected 293T; IRF6 antibody - middle region (ARP31995_P050) in r-IRF6 cells using Western Blot
Hum. Adult Placenta; Host:Rabbit. Target Name:IRF6. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; IRF6 antibody - middle region (ARP32670_P050) in Hum. Adult Placenta cells using Western Blot
Transfected 293T; WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; IRF6 antibody - middle region (ARP32670_P050) in Transfected 293T cells using Western Blot
Human 293T; WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: 293T cell lysate; IRF6 antibody - N-terminal region (ARP38738_P050) in Human 293T cells using Western Blot
Hum. Adult Placenta; Host: Rabbit. Target Name: SERPINA3 . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; IRF6 antibody - middle region (ARP38739_P050) in Hum. Adult Placenta cells using Western Blot
Transfected 293T; WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; IRF6 antibody - middle region (ARP38739_P050) in Transfected 293T cells using Western Blot
Human Kidney (formalin-fixed, paraffin-embedded) stained with IRF6 antibody AP22529PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Human Prostate (formalin-fixed, paraffin-embedded) stained with IRF6 antibody AP22529PU-N at 2.5 µg/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Antibody staining (0.2 µg/ml) of Human Ovary lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503159)
Anti-IRF6 mouse monoclonal antibody (TA503159) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6(RC201579).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IRF6 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.
HEK293T cells transfected with either RC201579 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IRF6 antibody(TA503159), and then analyzed by flow cytometry.
Anti-IRF6 mouse monoclonal antibody (TA503338) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6(RC201579).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.
HEK293T cells transfected with either RC201579 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IRF6 antibody(TA503338), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503345)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503345)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503345)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IRF6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503345)
Anti-IRF6 mouse monoclonal antibody (TA503345) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6(RC201579).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.
HEK293T cells transfected with either RC201579 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IRF6 antibody(TA503345), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.
Anti-IRF6 mouse monoclonal antibody (TA503382) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF6(RC201579).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF6 (RC201579, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF6.
HEK293T cells transfected with either RC201579 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IRF6 antibody(TA503382), and then analyzed by flow cytometry.
TA302518 staining (0.2µg/ml) of Human Ovary lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Transfected 293T
Immunohistochemistry with Placenta tissue at an antibody concentration of 5 ug/ml using anti-IRF6 antibody
WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
Host:Rabbit; Target Name:IRF6; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0ug/ml
WB Suggested Anti-IRF6 Antibody Titration: 2.5ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-IRF6 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: 293T cell lysate
Total cell lysate from HaCat cells (lane 1) and HeLa cells (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IRF6 antibody (clone 14B2C16). Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
HaCat cells were stained with purified anti-IRF6 antibody (clone 14B2C16), followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody and Alexa Fluorreg; 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
- Quick-Filters:
- Highly Validated (4)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| UM500074 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody,clone UMAB106 | |||||||
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Mouse | IgG2b | UMAB106 | Purified | Can, Hu, Mky | P |
0.1 ml /
€468.00
|
| OriGene Technologies, Inc. | |||||||
 +5 additional images |
Highly validated antibody!
|
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| UM570074 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody,clone UMAB106 | |||||||
|
Mouse | IgG2b | UMAB106 | Purified | Can, Hu, Mky | P |
30 µl /
€180.00
|
| OriGene Technologies, Inc. | |||||||
| +5 additional images |
Highly validated antibody!
|
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| TA503159 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody, clone OTI2C10 (formerly 2C10) | |||||||
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Mouse | IgG1 | OTI2C10 | Purified | Can, Hu, Rt | F, ICC/IF, P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
| +13 additional images |
Highly validated antibody!
|
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| TA503345 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody, clone OTI2A12 (formerly 2A12) | |||||||
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Mouse | IgG2b | OTI2A12 | Purified | Hu | F, ICC/IF, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +6 additional images |
Highly validated antibody!
|
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| TA302518 | IRF6 (C-term) antibody |
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| Goat Polyclonal Antibody against IRF6 | |||||||
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Goat | Aff - Purified | Hu, Ms | WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA503338 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody, clone OTI1D4 (formerly 1D4) | |||||||
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Mouse | IgG1 | OTI1D4 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| TA503369 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody, clone OTI1B9 (formerly 1B9) | |||||||
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Mouse | IgG2b | OTI1B9 | Purified | Hu | WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
|
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| TA503382 | IRF6 antibody |
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| IRF6 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3) | |||||||
 |
Mouse | IgG1 | OTI1G3 | Purified | Hu | F, ICC/IF, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
|
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| AP22529PU-N | IRF6 (455-467) antibody |
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 |
Goat | Aff - Purified | Elephant, Hu | E, P, WB |
50 µg /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP301579 | IRF6 |
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| Recombinant protein of human interferon regulatory factor 6 (IRF6) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
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| OriGene Technologies, Inc. | |||||