ISG20 / HEM45 antibody
Principal name
ISG20 / HEM45 antibody
Alternative names for ISG20 / HEM45 antibody
Interferon-stimulated gene 20 kDa protein, Promyelocytic leukemia nuclear body-associated protein ISG20, Estrogen-regulated transcript 45 protein
Ncbi ID
9606, NP_002192, NP_002192.2, NM_002201, NP_001106999.1, NM_020583
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)
Background of ISG20 / HEM45 antibody
- Kit Component:
- - KN308451G1, Isg20 gRNA vector 1 in pCas-Guide vector
- KN308451G2, Isg20 gRNA vector 2 in pCas-Guide vector
- KN308451D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Hao, Y., et al. J. Huazhong Univ. Sci. Technol. Med. Sci. 28(1):11-13(2008)
Horio, T., et al. FEBS Lett. 577 (1-2), 111-116 (2004)
Espert, L., et al. J. Biol. Chem. 278(18):16151-16158(2003)
Izmailova, E., et al. Nat. Med. 9(2):191-197(2003)
Nguyen, L.H., et al. Biochemistry 40(24):7174-7179(2001)
Immunofluorescence of monoclonal antibody to ISG20 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of ISG20 expression in transfected 293T cell line by ISG20 monoclonal antibody (M01), clone 1B2-3C9.Lane 1: ISG20 transfected lysate(20.4 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged ISG20 is approximately 0.1ng/ml as a capture antibody.
Human Daudi; WB Suggested Anti-ISG20 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: Daudi cell lysate. ISG20 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells.; ISG20 antibody - N-terminal region (ARP40392_T100) in Human Daudi cells using Western Blot
Western Blot analysis of ISG20 expression in transfected 293T cell line (H00003669-T01) by ISG20 MaxPab polyclonal antibody.Lane 1: ISG20 transfected lysate(19.91 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of ISG20 expression in transfected 293T cell line (H00003669-T01) by ISG20 MaxPab polyclonal antibody.Lane 1: ISG20 transfected lysate(20.40 KDa).Lane 2: Non-transfected lysate.
Human Jurkat; WB Suggested Anti-ISG20 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; ISG20 antibody - middle region (ARP40391_P050) in Human Jurkat cells using Western Blot
Immunoprecipitation of ISG20 transfected lysate using anti-ISG20 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ISG20 MaxPab mouse polyclonal antibody (B01) (H00003669-B01).
Western blot analysis of ISG20 / HEM45 Antibody (Center) Cat.-No AP52243PU-N in Ramos cell line lysates (35ug/lane). This demonstrates the ISG20 antibody detected the ISG20 protein (arrow).
WB Suggested Anti-ISG20 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate
WB Suggested Anti-ISG20 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Daudi cell lysate; ISG20 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52243PU-N | ISG20 / HEM45 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP303178 | ISG20 / HEM45 |
||||
| Recombinant protein of human interferon stimulated exonuclease gene 20kDa (ISG20) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY419473 | ISG20 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR218182L2V | Lenti ORF particles, Isg20 (GFP-tagged) - Mouse interferon-stimulated protein (Isg20), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR218182L1V | Lenti ORF particles, Isg20 (Myc-DDK-tagged) - Mouse interferon-stimulated protein (Isg20), transcript variant 1, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
