ITPK1 antibody
Principal name
ITPK1 antibody
Alternative names for ITPK1 antibody
Inositol-tetrakisphosphate 1-kinase, Inositol-triphosphate 5/6-kinase, Inositol 1,3,4-trisphosphate 5/6-kinase, Ins(1,3,4)P(3) 5/6-kinase
Ncbi ID
9606, NP_055031, NP_001136065, NP_001136065.1, NP_766172.1, NM_172584
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)
Background of ITPK1 antibody
- Kit Component:
- - KN308513G1, Itpk1 gRNA vector 1 in pCas-Guide vector
- KN308513G2, Itpk1 gRNA vector 2 in pCas-Guide vector
- KN308513D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Sun Y., et al. (2002) J Biol Chem. 277: 45759-45764Chamberlain P P., et al. (2007) J Biol Chem. 282: 28117-28125.
Western blot analysis of ITPK1 over-expressed 293 cell line, cotransfected with ITPK1 Validated Chimera RNAi ( Cat # H00003705-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ITPK1 monoclonal antibody (M01), clone 2C8 (Cat # H00003705-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot analysis of ITPK1 expression in transfected 293T cell line by ITPK1 monoclonal antibody (M01), clone 2C8.Lane 1: ITPK1 transfected lysate(35.6 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of purified MaxPab antibody to ITPK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to ITPK1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ITPK1 expression in transfected 293T cell line (H00003705-T01) by ITPK1 MaxPab polyclonal antibody.Lane 1: ITPK1 transfected lysate(34.54 KDa).Lane 2: Non-transfected lysate.
ITPK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ITPK1 expression in human liver.
Western Blot analysis of ITPK1 expression in transfected 293T cell line (H00003705-T01) by ITPK1 MaxPab polyclonal antibody.Lane 1: ITPK1 transfected lysate(35.60 KDa).Lane 2: Non-transfected lysate.
Immunoperoxidase of purified MaxPab antibody to ITPK1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]
Immunofluorescence of purified MaxPab antibody to ITPK1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of ITPK1 expression in transfected 293T cell line (H00003705-T01) by ITPK1 MaxPab polyclonal antibody.Lane 1: ITPK1 transfected lysate(34.54 KDa).Lane 2: Non-transfected lysate.
Human Heart; WB Suggested Anti-ITPK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human heart; ITPK1 antibody - N-terminal region (ARP54993_P050) in Human Heart cells using Western Blot
Western Blot analysis of ITPK1 expression in transfected 293T cell line (H00003705-T01) by ITPK1 MaxPab polyclonal antibody.Lane 1: ITPK1 transfected lysate(35.60 KDa).Lane 2: Non-transfected lysate.
ITPK1 MaxPab rabbit polyclonal antibody. Western Blot analysis of ITPK1 expression in human liver.
Immunoprecipitation of ITPK1 transfected lysate using anti-ITPK1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ITPK1 MaxPab mouse polyclonal antibody (B01) (H00003705-B01).
Human Spleen; WB Suggested Anti-ITPK1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; ITPK1 antibody - middle region (ARP54994_P050) in Human Spleen cells using Western Blot
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ITPK1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808613)(1:150)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ITPK1 (RC227205, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ITPK1.(1:2000)
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308666 diluted at 1:1000.
Sample (30 ug of whole cell lysate). A: A431 . 10% SDS PAGE. TA308666 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ITPK1(TA308666) antibody at 1:200 dilution.
Western blot analysis of extracts from HeLa cells, HepG2 cells and Jurkat cells, using ITPK1 antibody.The lane on the right is treated with the synthesized peptide.
WB Suggested Anti-ITPK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human heart
WB Suggested Anti-ITPK1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human Spleen
Western blot analysis on HeLa cell lysate using ITPK1 Antibody
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308666 | ITPK1 antibody |
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| Rabbit Polyclonal antibody to ITPK1 (inositol 1,3,4-triphosphate 5/6 kinase) | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, WB |
0.1 ml /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| TA314101 | ITPK1 antibody |
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| Rabbit polyclonal anti-ITPK1 antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA347681 | ITPK1 antibody |
||||||
| Rabbit Polyclonal Anti-ITPK1 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | WB |
0.1 mg /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA808613 | ITPK1 antibody |
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| ITPK1 mouse monoclonal antibody, clone OTI1F5 (formerly 1F5) | |||||||
 |
Mouse | IgG1 | OTI1F5 | Purified | Hu | P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP304537 | ITPK1 (transcript variant 1) |
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| Recombinant protein of human inositol 1,3,4-triphosphate 5/6 kinase (ITPK1), transcript variant 1 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP327205 | ITPK1 (transcript variant 2) |
||||
| Recombinant protein of human inositol 1,3,4-triphosphate 5/6 kinase (ITPK1), transcript variant 2 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR206652L2V | Lenti ORF particles, Itpk1 (GFP-tagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR206652L1V | Lenti ORF particles, Itpk1 (Myc-DDK-tagged) - Mouse inositol 1,3,4-triphosphate 5/6 kinase (Itpk1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||