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JIP-2 antibody

Principal name

JIP-2 antibody

Alternative names for JIP-2 antibody

JNK-interacting protein 2, MAPK8IP2, IB2, JIP2, PRKM8IPL, JIP2, Islet-brain-2, C-jun-amino-terminal kinase-interacting protein 2, JNK MAP kinase scaffold protein 2, Mitogen-activated protein kinase 8-interacting protein 2

SwissProt ID

Q13387 (Human), Q9ERE9 (Mouse)

Gene ID

23542 (MAPK8IP2), 60597 (Mapk8ip2)

Ncbi ID

9606, NP_036456.1, NP_036456, NP_068740.3, NM_021921, NM_016431

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C)

Background of JIP-2 antibody

c-Jun NH2-terminal kinases (JNKs) are distant members of the MAP kinase family. JNK1 is activated by dual phosphorylation at a Thr-Pro-Tyr motif in response to ultraviolet (UV) light, and it functions to phosphorylate c-Jun at amino terminal serine regulatory sites, Ser 63 and Ser 73, resulting in transcriptional activation. Two additional JNK family members have been identified as JNK2 and JNK3. JIP-1 (for JNK interacting protein-1) has been identified as a cytoplasmic inhibitor of JNK that retains JNK in the cytoplasm, thereby inhibiting JNK-regulated gene expression. Evidence suggests that JNK1 and JNK2 bind to JIP-1 with greater affinity than to ATF-2 and c-Jun, which are targets of the JNK signaling pathway. JIP-1 contains an amino terminal JNK binding domain and a carboxy terminal SH3 domain. ATF-2 and c-Jun also contain the JNK binding domain and are thought to compete with JIP-1 for JNK binding. Multiple splice variants of JIP-1, including JIP-1b, JIP-1c (also designated islet-brain 1 or IB-1), JIP-2a, JIP-2b and JIP-3, have been identified in brain.

Immunohistochemistry (IHC) analyzes of JIP-2 antibody (Cat.-No.: AP06724PU-N) in paraffin-embedded human brain tissue.

AP06724PU-N

Western blot (WB) analysis of JIP-2 antibody (Cat.-No.: AP06724PU-N) in extracts from raw264.7 cells.

AP06724PU-N

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using JIP2 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using JIP2 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from mouse brain cells, using JIP2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemical analysis of JIP2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of JIP2 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of JIP2 expression in Raw264.7 (A); mouse brain (B) whole cell lysates.

Adult rat brain membrane immunoblot

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Ttissue and transfected cell immunoblot: extracts of adult rat brain membrane RBM and COS cells transiently transfected with Flag-tagged JIP-1, JIP-2 or untagged Kv2.1 plasmid and probed with N135/37 (left), mouse anti-Flag (middle) and N52A/42 (right).

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Adult rat brain immunohistochemistry

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Immunoblot versus extracts from various WT (+/+) and IB2 KO (-/-) mouse brain regions probed with N135/37. Data courtesy of Mitchell Goldfarb (Hunter College) and reproduced with permission from the Journal of Neuroscience (2010 Giza et al, PMID 21048139).