JMJD4 antibody
Principal name
JMJD4 antibody
Alternative names for JMJD4 antibody
JmjC domain-containing protein 4, Jumonji domain-containing protein 4
Gene ID
65094 (JMJD4)
Ncbi ID
NP_075383, 9606, NP_001154937.1, NP_001154937, NM_023007, NM_001161465
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of JMJD4 antibody
- Kit Component:
- - KN221528G1, JMJD4 gRNA vector 1 in pCas-Guide vector
- KN221528G2, JMJD4 gRNA vector 2 in pCas-Guide vector
- KN221528D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Ota,T., (2004) Nat. Genet. 36 (1), 40-45
Human Jurkat; WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Jurkat cell lysate; JMJD4 antibody - C-terminal region (ARP39573_P050) in Human Jurkat cells using Western Blot
Western blot analysis of JMJD4 polyclonal antibody ( Cat # PAB2200 ) in HepG2 cell line lysate. JMJD4 ( N-term ) ( arrow ) was detected using the purified polyclonal antibody.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with JMJD4 polyclonal antibody ( Cat # PAB2201 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
Western blot analysis of JMJD4 polyclonal antibody ( Cat # PAB2201 ) in Hela and mouse kidney cell line lysate. JMJD4 ( C-term ) ( arrow ) was detected using the purified polyclonal antibody.
Western blot analysis of anti-JMJD4 (N-term) Pab in HepG2 cell line lysate. JMJD4 (N-term)(arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with JMJD4 (C-term) (Cat.#AP11045PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-JMJD4 (C-term) Pab in Hela and mouse kidney cell line lysate. JMJD4 (C-term)(arrow) was detected using the purified Pab.
Human Heart; WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human heart; JMJD4 antibody - N-terminal region (ARP33301_P050) in Human Heart cells using Western Blot
Human 721_B; Host:Rabbit. Target Name:JMJD4. Sample Tissue:Human 721_B. Antibody Dilution: 1.0ug/ml. JMJD4 is supported by BioGPS gene expression data to be expressed in 721_B.; JMJD4 antibody - N-terminal region (ARP33301_P050) in Human 721_B cells using Western Blot
Hum. Fetal Liver; Host:Rabbit. Target Name:JMJD4. Sample Tissue:Human Fetal Liver. Antibody Dilution: 1.0ug/ml.; JMJD4 antibody - N-terminal region (ARP33301_P050) in Hum. Fetal Liver cells using Western Blot
Human 293T; WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate. JMJD4 is supported by BioGPS gene expression data to be expressed in HEK293T.; JMJD4 antibody - N-terminal region (ARP39572_P050) in Human 293T cells using Western Blot
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
Human ACHN; WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: ACHN cell lysate; JMJD4 antibody - C-terminal region (ARP33300_P050) in Human ACHN cells using Western Blot
Human 293T; Host:Rabbit. Target Name:JMJD4. Sample Tissue:Human 293T. Antibody Dilution: 1.0ug/ml. JMJD4 is supported by BioGPS gene expression data to be expressed in HEK293T.; JMJD4 antibody - C-terminal region (ARP33300_P050) in Human 293T cells using Western Blot
Immunohistochemistry of JMJD4 in rat spleen tissue with JMJD4 antibody at 2.5 ug/ml.
Immunofluorescence of JMJD4 in rat spleen tissue with JMJD4 antibody at 5 ug/mL.
Western blot analysis of JMJD4 in human spleen tissue lysate with JMJD4 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.
WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: ACHN cell lysate
Host:Rabbit; Target Name:JMJD4; Sample Tissue:Human 293T; Antibody Dilution: 1.0 ug/mlJMJD4 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: Human heart
Host:Rabbit; Target Name:JMJD4; Sample Tissue:Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
Host:Rabbit; Target Name:JMJD4; Sample Tissue:Human 721_B; Antibody Dilution: 1.0 ug/mlJMJD4 is supported by BioGPS gene expression data to be expressed in 721_B
WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: 293T cell lysateJMJD4 is supported by BioGPS gene expression data to be expressed in HEK293T
WB Suggested Anti-JMJD4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA306833 | JMJD4 antibody |
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| Rabbit Polyclonal JMJD4 Antibody | |||||||
 |
Rabbit | IgG | Aff - Purified | Hu, Rt | E, P, WB |
0.1 mg /
€415.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
|||||||
| AP11044PU-N | JMJD4 (N-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| AP11045PU-N | JMJD4 (C-term) antibody |
||||||
 |
Rabbit | Ig | Purified | Hu, Ms | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
| AP23900PU-N | JMJD4 (N-term) antibody |
||||||
 |
Rabbit | Purified | Hu, Rt | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
| +1 additional image |
|||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY431386 | JMJD4 overexpression lysate |
|
 |
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |