Josephin-2 antibody
Principal name
Josephin-2 antibody
Alternative names for Josephin-2 antibody
JOSD2, Josephin domain-containing protein 2
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB)
Background of Josephin-2 antibody
- Kit Component:
- - KN308559G1, Josd2 gRNA vector 1 in pCas-Guide vector
- KN308559G2, Josd2 gRNA vector 2 in pCas-Guide vector
- KN308559D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Albrecht,M., (2003) Proteins 50 (2), 355-370
Transfected 293T; WB Suggested Anti-JOSD2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; JOSD2 antibody - N-terminal region (ARP52586_P050) in Transfected 293T cells using Western Blot
Western blot analysis of Josephin-2 Antibody (N-term) Cat.-No AP52274PU-N in mouse Neuro-2a cell line lysates (35ug/lane). This demonstrates the JOSD2 antibody detected the JOSD2 protein (arrow).
Flow cytometric analysis of Neuro-2a cells using Josephin-2 Antibody (N-term) Cat.-No AP52274PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
WB Suggested Anti-JOSD2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| AP52274PU-N | Josephin-2 (N-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu, Ms | WB |
0.4 ml /
€415.00
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| OriGene Technologies GmbH | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309025 | Josephin-2 |
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| Recombinant protein of human Josephin domain containing 2 (JOSD2) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR200279L2V | Lenti ORF particles, Josd2 (GFP-tagged) - Mouse Josephin domain containing 2 (cDNA clone MGC:25921 IMAGE:4224952), 200 uL, >10^7 TU/mL |
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 |
200 µl /
€840.00
|
|
| OriGene Technologies, Inc. | ||
| MR200279L1V | Lenti ORF particles, Josd2 (Myc-DDK-tagged) - Mouse Josephin domain containing 2 (cDNA clone MGC:25921 IMAGE:4224952), 200 uL, >10^7 TU/mL |
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|
200 µl /
€740.00
|
|
| OriGene Technologies, Inc. | ||