KARS antibody
Principal name
KARS antibody
Alternative names for KARS antibody
Lysyl-tRNA synthetase, KIAA0070, LysRS, Lysine-tRNA ligase
Ncbi ID
9606, NP_005539, 10090, NP_001123561, NP_001123561.1, NM_005548, NM_001130089, NP_444322.1, BC027356, NM_053092
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F)
Background of KARS antibody
- Kit Component:
- - KN308582G1, Kars gRNA vector 1 in pCas-Guide vector
- KN308582G2, Kars gRNA vector 2 in pCas-Guide vector
- KN308582D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
McLaughlin, H.M., et al. Am. J. Hum. Genet. 87(4):560-566(2010)
Kepp, O., et al. Cell Cycle 9(15):3072-3077(2010)
Segat, L., et al. Vaccine 28(10):2201-2206(2010)
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Kawamata, H., et al. J. Biol. Chem. 283(42):28321-28328(2008)
Human Intestine; Rabbit Anti-KARS Antibody. Catalog Number: ARP40589. Paraffin Embedded Tissue: Human Intestine. Cellular Data: Epithelial cells of intestinal villas. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; KARS antibody - C-terminal region (ARP40589_P050) in Human Intestine cells using Immunohistochemistry
Human HepG2; WB Suggested Anti-KARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate. KARS is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; KARS antibody - C-terminal region (ARP40589_P050) in Human HepG2 cells using Western Blot
KARS, 63-597aa, Human, His-tagged,
Western blot analysis of KARS Antibody (N-term) Cat.-No AP52290PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the KARS antibody detected the KARS protein (arrow).
Western blot analysis of KARS (arrow) using KARS Antibody (N-term) Cat.-No AP52290PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KARS gene.
Flow cytometric analysis of 293 cells using KARS Antibody (N-term) Cat.-No AP52290PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using KARS antibody Cat.-No AM06252SU-N
Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using KARSantibody Cat.-No AM06252SU-N
Western blot analysis using KARS antibody Cat.-No AM06252SU-N against truncated Trx-KARS recombinant protein (Lane 1), truncated MBP-KARS (aa90-174) and full length KARS (aa1-188) transfected CHO-K1 cell lysate (Lane 3).
WB Suggested Anti-KARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: HepG2 cell lysate; KARS is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells
Rabbit Anti-KARS Antibody; Paraffin Embedded Tissue: Human Intestine; Cellular Data: Epithelial cells of intestinal villas; Antibody Concentration: 4.0-8.0 ug/ml; Magnification: 400X
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-6: K562 cells, A549 cells, HT29 cells, 293T cells, Hela cells, Jurkat cells, Primary antibody: (KARS Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using (KARS Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (KARS Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA350109 | KARS antibody |
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| Rabbit Polyclonal Anti-KARS Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AM06252SU-N | KARS antibody |
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 |
Mouse | IgG1 | 8G12C1 | Ascites | Hu | P, WB |
0.1 ml /
€380.00
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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| AP52290PU-N | KARS (N-term) antibody |
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 |
Rabbit | Ig | Purified | Hu | F, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP300311 | KARS (transcript variant 2) |
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| Recombinant protein of human lysyl-tRNA synthetase (KARS), transcript variant 2 | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY401702 | KARS overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY427157 | KARS overexpression lysate |
|
|
0.1 mg /
€315.00
|
|
| OriGene Technologies, Inc. |
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR209528L2V | Lenti ORF particles, Kars (GFP-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,240.00
|
|
| OriGene Technologies, Inc. | ||
| MR217682L2V | Lenti ORF particles, Kars (GFP-tagged) - Mouse lysyl-tRNA synthetase (Kars), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,330.00
|
|
| OriGene Technologies, Inc. | ||
| MR209528L1V | Lenti ORF particles, Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (cDNA clone MGC:37041 IMAGE:4950333), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,110.00
|
|
| OriGene Technologies, Inc. | ||
| MR217682L1V | Lenti ORF particles, Kars (Myc-DDK-tagged) - Mouse lysyl-tRNA synthetase (Kars), transcript variant 2, 200 uL, >10^7 TU/mL |
|
|
200 µl /
€1,190.00
|
|
| OriGene Technologies, Inc. | ||