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KIF11 antibody

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Principal name

KIF11 antibody

Alternative names for KIF11 antibody

KIF11, EG5, KNSL1, TRIP5, Kinesin-like protein KIF11, Kinesin-related motor protein Eg5, Kinesin-like spindle protein HKSP, Thyroid receptor-interacting protein 5, TRIP-5, Kinesin-like protein 1

SwissProt ID

P52732 (Human), Q6P9P6 (Mouse), Q6S001 (Dicdi)

Gene ID

3832 (KIF11), 16551 (Kif11)

Ncbi ID

9606, NP_004514, NP_004514.2, NM_004523, NP_034745.1, NM_010615

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Gt (Goat), Hst (Hamster), Sh (Sheep)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of KIF11 antibody

Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2.
Structure: Kinesin-like protein family, BimC subfamily, kinesin motor catalytic, coiled-coil, Smc domains; 119 kD
Distribution: Centrosomes, spindle microtubules, intracellular bridge. 
Function: Motor protein required for establishing bipolar spindle. 
Modification: Phosphorylation.
Regulation: Phosphorylation on Thr927 by Cdc2 allows association with spindle apparatus.
Interaction: Thyroid hormone receptor in presence of thyroid hormone, Cdc2.

General readings

Tcherniuk,S., et.al., Biochem. Pharmacol. 79 (6), 864-872 (2010)
Parke,C.L., et.al., J. Biol. Chem. 285 (8), 5859-5867 (2010)

PAB12408 - KIF11
Staining of KIF11 on HeLa cells fixed in 3.5% paraformaldehyde using KIF11 polyclonal antibody ( Cat # PAB12408 ). Cytoplasmic staining during interphase and spindle staining during mitosis.
PAB12408 - KIF11
Western blot analysis of KIF11 on lysates of cells that were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA using KIF11 polyclonal antibody ( Cat # PAB12408 ).
AP20253PU-N - KIF11
Immunohistochemistry analyzes of Eg5 antibody (AP20253PU-N) in paraffin-embedded human lung carcinoma tissue.
AP20253PU-N - KIF11
Western blot analysis of Eg5 antibody (AP20253PU-N) in extracts from COLO205 cells.
A1081-1 - KIF11
Western blot analysis of extracts from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) Antibody. The lane on the right is treated with the synthesized peptide.
B1081-1 - KIF11
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using KIF11/Eg5 (Ab-927) Antibody. The picture on the right is treated with the synthesized peptide.
B1081-1 - KIF11
Immunofluorescence analysis of HeLa cells, using KIF11/Eg5 (Ab-927) Antibody. The picture on the right is treated with the synthesized peptide.
B1081-1 - KIF11
Western blot analysis of extracts from Jurkat/HepG2/COS cells, using KIF11/Eg5 (Ab-927) Antibody. The lane on the right is treated with the synthesized peptide.
AM26646AF-N - KIF11

Immunoflourescence detection of KIF11 on acetone fixed HEp-2 cells with AM26646AF-N.


AM26646AF-N - KIF11

Immunoprecipitation of KIF11 from 293T with AM26646AF-N (1) or normal mouse IgG (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26646AF-N.


AM26647AF-N - KIF11

Western blot analysis of KIF11 expression in 293T using AM26647AF-N.


AM26647AF-N - KIF11

Immunoprecipitation of KIF11 from 293T with AM26647AF-N (1) or normal mouse IgG (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with AM26647AF-N.


AM33111PU-S - KIF11
Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Eg5 (Clone 10C7/Eg5) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
TP318842 - KIF11
TA301478 - KIF11
Western Blot: Eg5 Antibody TA301478 - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11.
TA301478 - KIF11
Staining of HeLa cells fixed in 3.5% paraformaldehyde (1:5,000). Centrosome staining during interphase and spindle pole staining during mitosis.
TA325092 - KIF11
Western blot analysis of KIF11 Antibody (N-term) (Cat. #TA325092) in CHO cell line lysates (35ug/lane). KIF11 (arrow) was detected using the purified Pab.
TA325092 - KIF11
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KIF11 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
TA325092 - KIF11
KIF11 Antibody (N-term) (Cat.#TA325092) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TA327995 - KIF11
Hela cells were treated with 300 uM mimosine for 16 hrs, then placed in complete media (lane 1) or media containing 200 ng/ml nocodazole for an additional 18hrs. Cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit antibody against phosphorylated Eg5 (Thr927). Lane 1, Mimosine only treated Hela cells; Lane 2, mimosine and nocodazole treated Hela.
TA346987 - KIF11
Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.
TA346987 - KIF11
Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).
TA346987 - KIF11
Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.
TA312160 - KIF11
Western blot analysis of extracts from COLO205 cells, using KIF11/Eg5 (Phospho-Thr926) antibody.The lane on the right is treated with the synthesized peptide.
TA312160 - KIF11
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using KIF11/Eg5 (Phospho-Thr926) antibody.The picture on the right is treated with the synthesized peptide.
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Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KIF11
  Recombinant protein of human kinesin family member 11 (KIF11)  
KIF11 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.
 

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