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KLF15 antibody

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Principal name

KLF15 antibody

Alternative names for KLF15 antibody

KLF15, KKLF, Krueppel-like factor 15, Kidney-enriched krueppel-like factor, CKLF, Cardiovascular Krueppel-like factor

SwissProt ID

Q9EPW2 (Mouse), Q9UIH9 (Human)

Gene ID

28999 (KLF15), 66277 (Klf15)

Ncbi ID

NP_054798, 9606, NP_054798.1, NM_014079, NP_075673.1, NM_023184

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of KLF15 antibody

The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84% identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.

General readings

Uchida, S., et al., (2000) Mol. Cell. Biol. 20 (19), 7319-7331

ARP32587_T100 - KLF15
Human Liver; KLF15 antibody - N-terminal region (ARP32587_T100) in Human Liver cells using Immunohistochemistry
ARP32587_T100 - KLF15
Human HepG2; WB Suggested Anti-KLF15 Antibody Titration: 1.0-2.0ug/ml. Positive Control: HepG2 cell lysate; KLF15 antibody - N-terminal region (ARP32587_T100) in Human HepG2 cells using Western Blot
PAB6181 - KLF15
KLF15 polyclonal antibody ( Cat # PAB6181 ) staining ( 0.3 µg/ml ) of HepG2 lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
H00028999-B01 - KLF15
Immunofluorescence of purified MaxPab antibody to KLF15 on HepG2 cell. [antibody concentration 10 ug/ml]
H00028999-B01 - KLF15
Western Blot analysis of KLF15 expression in transfected 293T cell line (H00028999-T01) by KLF15 MaxPab polyclonal antibody.Lane 1: KLF15 transfected lysate(45.76 KDa).Lane 2: Non-transfected lysate.
H00028999-D01P - KLF15
Western Blot analysis of KLF15 expression in transfected 293T cell line (H00028999-T02) by KLF15 MaxPab polyclonal antibody.Lane 1: KLF15 transfected lysate(44.00 KDa).Lane 2: Non-transfected lysate.
ARP31900_P050 - KLF15
Human Spleen; WB Suggested Anti-KLF15 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human Spleen; KLF15 antibody - middle region (ARP31900_P050) in Human Spleen cells using Western Blot
TP307523 - KLF15
TA302522 - KLF15
TA302522 staining (0.3µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
TA310150 - KLF15
Western blot analysis of fetal liver lysates with anti-KLF15 antibody (1:1000). This antibody, under reducing conditions, identified a band of ~65kDa.
TA330618 - KLF15
WB Suggested Anti-KLF15 Antibody Titration: 1.0-2.0ug/ml; Positive Control: HepG2 cell lysate
TA330618 - KLF15
Human Liver
TA344498 - KLF15
WB Suggested Anti-Klf15 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Brain
TA349372 - KLF15
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (KLF15 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA349372 - KLF15
The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using (KLF15 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200)
TA329447 - KLF15
WB Suggested Anti-KLF15 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human Spleen

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLF15 (N-term) antibody
  Goat Polyclonal Antibody against KLF15  
TA302522 staining (0.3µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

KLF15 antibody
  Rabbit anti-KLF15 polyclonal antibody  
Western blot analysis of fetal liver lysates with anti-KLF15 antibody (1:1000). This antibody, under reducing conditions, identified a band of ~65kDa. Rabbit Purified Hu WB
0.1 ml / €220.00
  OriGene Technologies, Inc.

KLF15 antibody
  Rabbit Polyclonal Anti-KLF15 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (KLF15 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

CRISPR

Catalog No. Species Donor Vector  

Klf15 - mouse gene knockout kit via CRISPR
Klf15 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Klf15 - mouse gene knockout kit via CRISPR
Klf15 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Klf15 - mouse gene knockout kit via CRISPR
Klf15 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

KLF15
  Recombinant protein of human Kruppel-like factor 15 (KLF15)  
KLF15 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

KLF15 overexpression lysate
KLF15 overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Klf15 (GFP-tagged) - Mouse Kruppel-like factor 15 (Klf15), 200 uL, >10^7 TU/mL
Lenti ORF particles, Klf15 (GFP-tagged) - Mouse Kruppel-like factor 15 (Klf15), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15), 200 uL, >10^7 TU/mL
Lenti ORF particles, Klf15 (Myc-DDK-tagged) - Mouse Kruppel-like factor 15 (Klf15), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. CRISPR (3)
  2. Lysate (1)
  3. Mouse Lenti ORF Particle (2)
  4. Primary Antibody (3)
  5. Protein | Growth Factor (1)
Clonality
  1. Polyclonal (3)
Target
  1. KLF15 (4)
Property
  1. N-term (1)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Porcine (Por) (1)
  4. Rat (Rt) (1)
Applications
  1. Western blot / Immunoblot (WB) (2)
  2. Enzyme Immunoassay (E) (1)
  3. Paraffin Sections (P) (1)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (2)
  2. Goat (1)
Images
  1. 1 image (4)
Images: Applications
  1. Western blot / Immunoblot (WB) (2)
  2. IHC (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Species (CRISPR)
  1. Mouse (3)
Donor Vector
  1. GFP-Puro (1)
  2. Luciferase-Puro (1)
  3. mBFP-Neo (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
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  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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