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KLF5 antibody

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Principal name

KLF5 antibody

Alternative names for KLF5 antibody

KLF5, BTEB2, CKLF, IKLF, Krueppel-like factor 5, Colon krueppel-like factor, Transcription factor BTEB2, BTE-binding protein 2, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Basic transcription element-binding protein 2

SwissProt ID

Q13887 (Human), Q9Z0Z7 (Mouse)

Gene ID

688 (KLF5), 12224 (Klf5)

Ncbi ID

9606, NP_001721, NP_001721.2, NP_033899.2, NM_001730

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of KLF5 antibody

KLF5 (Krueppel-like factor 5) is a protein that in humans is encoded by the KLF5 gene. It is also known as BASIC TRANSCRIPTION ELEMENT-BINDING PROTEIN 2 or BTEB2. This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Suske et al. (2005)stated that the human KLF5 gene maps to chromosome 13q21.33, and the mouse Klf5 gene to chromosome 14E2.1. Sogawa et al. (1993)isolated a cDNA encoding BTEB2. The predicted 219-amino acid protein contains 3 consecutive zinc finger motifs near the C terminus. The zinc finger domains were 59% and 64% identical to those of Sp1 (189906) and BTEB1, respectively. Both BTEB2 and Sp1 have a short basic region preceding the zinc finger motifs that may play an auxiliary role in DNA binding. The N-terminal region of BTEB2 is proline rich. Recombinant BTEB2 protein and Sp1 showed very similar DNA-binding specificity in vitro. When expressed in mammalian cells, BTEB2 activated the transcription of a reporter gene fused to a GC box-containing promoter. Sogawa et al. (1993) also identified the rat BTEB2 homolog. Northern blot analysis of rat tissues revealed that BTEB2 is expressed only in the testis and placenta.

H00000688-M01 - KLF5
Detection limit for recombinant GST tagged KLF5 is approximately 0.3ng/ml as a capture antibody.
AP17518PU-N - KLF5
Western blot analysis of KLF5 antibody (Center) (AP17518PU-N) in CEM cell line lysates (35ug/lane). KLF5 (arrow) was detected using the purified Pab.
TA323126 - KLF5
Predicted band size: 51 kDa . Positive control: Jurkat cell and human ovarian cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lane1: Jurkat cell lysate Lane2: Human ovarian cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
TA323126 - KLF5
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human ovarian cancer tissue. Recommended dilution: 1/100-300 The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using KLF5 antibody at dilution 1/120, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA323127 - KLF5
Predicted band size: 51 kDa . Positive control: Human lymphoma, ovarian and colon cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lane1: Human lymphoma tissue lysate Lane2: Human ovarian cancer tissue lysate Lane3: Human colon cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds)
TA324928 - KLF5
KLF5 Antibody (C-term) (Cat. #TA324928) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).
TA324928 - KLF5
KLF5 Antibody (C-term) (Cat.#TA324928) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
TA324928 - KLF5
IF image of U251 cell stained with KLF5 Antibody (C-term)(Cat#TA324928).U251 cells were incubated with KLF5 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
TA324928 - KLF5
KLF5 Antibody (C-term) (Cat. #TA324928) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

4 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

KLF5 antibody
  Anti-KLF5 Rabbit Polyclonal Antibody  
Predicted band size: 51 kDa . Positive control: Jurkat cell and human ovarian cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lane1: Jurkat cell lysate Lane2: Human ovarian cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/1700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

KLF5 antibody
  Anti-KLF5 Rabbit Polyclonal Antibody  
Predicted band size: 51 kDa . Positive control: Human lymphoma, ovarian and colon cancer tissue lysate. Recommended dilution: 1/500-2000. (Gel: %SDS-PAGE Lane1: Human lymphoma tissue lysate Lane2: Human ovarian cancer tissue lysate Lane3: Human colon cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/350 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 20 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.

KLF5 antibody
  Rabbit polyclonal KLF5 Antibody (C-term)  
KLF5 Antibody (C-term) (Cat. #TA324928) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow). Rabbit IgG Purified Hu, Ms F, ICC/IF, P, WB
0.4 ml / €415.00
  OriGene Technologies, Inc.
+3 additional images

KLF5 antibody
Western blot analysis of KLF5 antibody (Center) (AP17518PU-N) in CEM cell line lysates (35ug/lane). KLF5 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €415.00
  OriGene Technologies GmbH

4 Item(s)

per page

Available Filters

Product Type
  1. Primary Antibody (4)
Clonality
  1. Polyclonal (4)
Target
  1. KLF5 (4)
Reactivity
  1. Human (Hu) (4)
  2. Mouse (Ms) (3)
  3. Rat (Rt) (2)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Paraffin Sections (P) (2)
  4. Flow cytometry (F) (1)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (1)
Presentation
  1. Purified (3)
Host
  1. Rabbit (4)
Images
  1. 1 image (4)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. IHC (2)
  3. Immunofluorescence (IF) (1)
Manufacturer
  1. OriGene Technologies (4)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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