KLHL3 antibody
Principal name
KLHL3 antibody
Alternative names for KLHL3 antibody
KLHL-3, Kelch-like protein 3, KIAA1129
Gene ID
26249 (KLHL3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of KLHL3 antibody
KLHL3 protein contains a poxvirus and zinc finger domain at the N-terminus and six tandem repeats (kelch repeats) at the C-terminus. At the amino acid level, KLHL3 shares 77% similarity with Drosophila kelch and 89% similarity with Mayven (KLHL2), another human kelch homolog. At least three isoforms are produced and may be the result of alternative promoter usage. The KLHL3 maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, no inactivating mutations of KLHL3 could be detected in malignant myeloid disorders with loss of 5q.
General readings
Lai,F., et al., (2000) Genomics 66 (1), 65-75
Human HepG2; WB Suggested Anti-KLHL3 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; KLHL3 antibody - N-terminal region (ARP39276_T100) in Human HepG2 cells using Western Blot
Western Blot analysis of KLHL3 expression in transfected 293T cell line (H00026249-T01) by KLHL3 MaxPab polyclonal antibody.Lane 1: KLHL3 transfected lysate(33.11 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of KLHL3 expression in transfected 293T cell line (H00026249-T01) by KLHL3 MaxPab polyclonal antibody.Lane 1: KLHL3 transfected lysate(33.11 KDa).Lane 2: Non-transfected lysate.
Human HepG2; WB Suggested Anti-KLHL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; KLHL3 antibody - middle region (ARP39277_P050) in Human HepG2 cells using Western Blot
Immunohistochemistry of paraffin-embedded human tonsillitis slide using 16951-1-AP( KLHL3 Antibody) at dilution of 1:50
Immunohistochemical of paraffin-embedded human liver using 16951-1-AP(KLHL3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using 16951-1-AP(KLHL3 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis slide using 16951-1-AP( KLHL3 Antibody) at dilution of 1:50
HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:800
HepG2 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:1000
HL-60 cells were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:300
Jurkat cells were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:1000
Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:200
Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:1000
Rat liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:800
Human liver tissue were subjected to SDS PAGE followed by western blot with 16951-1-AP(KLHL3 antibody) at dilution of 1:200
IP Result of anti-KLHL3 (IP:16951-1-AP, 4ug; Detection:16951-1-AP 1:700) with HepG2 cells lysate 2800ug.
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using KLHL3(GTX115809) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX115809 diluted at 1:10000
Human brain stem; . Sample Type :. Human brain stem cells. Primary Antibody Dilution :. 1:500. Secondary Antibody :. Goat anti-rabbit Alexa-Fluor 594. Secondary Antibody Dilution :. 1:1000. Color/Signal Descriptions :. KLHL3: Red DAPI:Blue. Gene Name :. KLHL3. Submitted by :. Dr. Yuzhi Chen, University of Arkansas for Medical Science; KLHL3 antibody - N-terminal region (ARP34561_P050) in Human brain stem cells using Immunohistochemistry
Human THP-1; WB Suggested Anti-KLHL3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: THP-1 cell lysate; KLHL3 antibody - N-terminal region (ARP34561_P050) in Human THP-1 cells using Western Blot
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA315317 | KLHL3 antibody |
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| Rabbit polyclonal anti-KLHL3 antibody | |||||||
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Rabbit | IgG | Purified | Hu | E, ICC/IF, P, WB |
0.1 ml /
€335.00
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|
| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP318393 | KLHL3 |
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| Recombinant protein of human kelch-like 3 (Drosophila) (KLHL3) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY413773 | KLHL3 overexpression lysate |
|
 |
0.1 mg /
€495.00
|
|
| OriGene Technologies, Inc. |