Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » Lactadherin antibody

Lactadherin antibody

(reset)

Principal name

Lactadherin antibody

Alternative names for Lactadherin antibody

MFGE8, Milk fat globule-EGF factor 8, HMFG, Breast epithelial antigen BA46, MFGM

SwissProt ID

P21956 (Mouse), P70490 (Rat), P79385 (Pig), Q08431 (Human), Q95114 (Bovin)

Gene ID

4240 (MFGE8), 17304 (Mfge8)

Ncbi ID

NP_001108086.1, 9606, NP_001108086, 10090, NP_005919, NM_001045489, NM_001114614

Available reactivities

Hu (Human), Ms (Mouse)

Available hosts

Mouse, Rabbit, Hamster

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F)

Background of Lactadherin antibody

MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al. (1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al. (2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al. (2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE)-dependent mechanism.

General readings

Yamaguchi, H., et al. Eur. J. Immunol. 40(6):1778-1785(2010)
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009)
Hu, C.Y., et al. Lupus 18(8):676-681(2009)
Fu, Z., et al. Circ. Res. 104(12):1337-1346(2009)
Raymond, A., et al. J. Cell. Biochem. 106(6):957-966(2009)

H00004240-M09 - Lactadherin
Detection limit for recombinant GST tagged MFGE8 is approximately 0.3ng/ml as a capture antibody.
H00004240-M03 - Lactadherin
Detection limit for recombinant GST tagged MFGE8 is approximately 0.3ng/ml as a capture antibody.
H00004240-B01P - Lactadherin
MFGE8 MaxPab polyclonal antibody. Western Blot analysis of MFGE8 expression in human pancreas.
H00004240-B01P - Lactadherin
Western Blot analysis of MFGE8 expression in transfected 293T cell line (H00004240-T01) by MFGE8 MaxPab polyclonal antibody.Lane 1: MFGE8 transfected lysate(42.57 KDa).Lane 2: Non-transfected lysate.
H00004240-D01P - Lactadherin
Western Blot analysis of MFGE8 expression in transfected 293T cell line (H00004240-T02) by MFGE8 MaxPab polyclonal antibody.Lane 1: MFGE8 transfected lysate(43.10 KDa).Lane 2: Non-transfected lysate.
H00004240-D01 - Lactadherin
Immunoprecipitation of MFGE8 transfected lysate using anti-MFGE8 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MFGE8 purified MaxPab mouse polyclonal antibody (B01P) (H00004240-B01P).
AP52057PU-N - Lactadherin
Western blot analysis of  Lactadherin Antibody (C-term) Cat.-No AP52057PU-N in Hela cell line lysates (35ug/lane).
AP52057PU-N - Lactadherin
Flow cytometric analysis of Hela cells using  Lactadherin Antibody (C-term) Cat.-No AP52057PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
AM31920PU-N - Lactadherin
Human Lung, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM31920PU-N - Lactadherin
Detection limit for recombinant GST tagged MFGE8 is approximately 0.3ng/ml as a capture antibody.
AM31920PU-N - Lactadherin
Immunoprecipitation of MFGE8 transfected lysate using anti-MFGE8 monoclonal antibody and Protein A magnetic bead, immunoblotted with MFGE8 rabbit polyclonal antibody.
AM26495AF-N - Lactadherin
Flow cytometric analysis of MFG-E8 binding to apoptotic Jurkat cells. Apoptotic Jurkat cells induced by anti-Fas (CH-11/code no. SY001) were incubated with recombinat MFG-E8. MFG-E8 bound to the apoptotic cells was detected with AM26495AF-N. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26495AF-N to the cells.
AM26495AF-N - Lactadherin
Immunoprecipitation of MFG-E8 from culture supernatant of mouse bone marrow cells with AM26495AF (1) or hamster IgG (2). After immunoprecipitated with the antibody, immunocomplex was resolved on SDS-PAGE and immunoblotted with Clone 18A2-G10.
AM26517AF-N - Lactadherin

Western blot analysis of MFG-E8 expression on mouse epididymides (1), recombinant HPC4 tagged mouse MFG-E8 short form (2) and long form (3) using AM26517AF-N.


CEK1266 - Human MFG-E8 ELISA Kit
Representative Standard Curve
CEK1529 - Mouse MFG-E8 ELISA Kit
Representative Standard Curve
TP317163 - Lactadherin
TA317012 - Lactadherin
Anti-MFGE8 / MFG-E8 antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

10 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Lactadherin antibody
  Lactadherin / MFGE8 Mouse Monoclonal (B656M) Antibody  
Anti-MFGE8 / MFG-E8 antibody IHC of human placenta, trophoblast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Mouse IgG1 B656M Purified Hu C, ICC/IF, WB
50 µg / €425.00
  OriGene Technologies, Inc.

Lactadherin antibody
Flow cytometric analysis of MFG-E8 binding to apoptotic Jurkat cells. Apoptotic Jurkat cells induced by anti-Fas (CH-11/code no. SY001) were incubated with recombinat MFG-E8. MFG-E8 bound to the apoptotic cells was detected with AM26495AF-N. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of AM26495AF-N to the cells. Hamster IgG 2422 Azide Free Ms F, IP
0.1 mg / €400.00
  OriGene Technologies GmbH
+1 additional image

Lactadherin antibody
Western blot analysis of MFG-E8 expression on mouse epididymides (1), recombinant HPC4 tagged mouse MFG-E8 short form (2) and long form (3) using AM26517AF-N. Hamster IgG 18A2-G10 Azide Free Ms C, WB
0.1 mg / €400.00
  OriGene Technologies GmbH

Lactadherin antibody
Human Lung, Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1E7 Purified Hu E, IP, P, WB
50 µg / €475.00
  OriGene Technologies GmbH
+2 additional images

Lactadherin (C-term) antibody
Western blot analysis of  Lactadherin Antibody (C-term) Cat.-No AP52057PU-N in Hela cell line lysates (35ug/lane). Rabbit Ig Aff - Purified Hu F, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Lactadherin antibody
Lactadherin Mouse IgG1 MFG-06 Purified Hu C, E, ICC/IF, P, WB
0.1 mg / €190.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Lactadherin (transcript variant 1)
  Recombinant protein of human milk fat globule-EGF factor 8 protein (MFGE8), transcript variant 1  
Lactadherin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

ELISA Kits

Catalog No.    

Human MFGE8/Lactadherin ELISA Kit
Human MFGE8/Lactadherin ELISA Kit  
1 kit / €500.00
  OriGene Technologies, Inc.

Mouse MFGE8/Lactadherin ELISA Kit
Mouse MFGE8/Lactadherin ELISA Kit  
1 kit / €500.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Mfge8 (Myc-DDK-tagged) - Mouse milk fat globule-EGF factor 8 protein (Mfge8), transcript variant 2, 200 uL, >10^7 TU/mL
Lenti ORF particles, Mfge8 (Myc-DDK-tagged) - Mouse milk fat globule-EGF factor 8 protein (Mfge8), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

10 Item(s)

per page

Available Filters

Product Type
  1. ELISA Kit (2)
  2. Mouse Lenti ORF Particle (1)
  3. Primary Antibody (6)
  4. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (5)
  2. Polyclonal (1)
Target
  1. Lactadherin (7)
Property
  1. C-term (1)
Reactivity
  1. Human (Hu) (5)
  2. Mouse (Ms) (3)
Applications
  1. Western blot / Immunoblot (WB) (5)
  2. Frozen sections (C) (3)
  3. Enzyme Immunoassay (E) (2)
  4. Flow cytometry (F) (2)
  5. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  6. Immunoprecipitation (IP) (2)
  7. Paraffin Sections (P) (2)
Presentation
  1. Purified (3)
  2. Azide Free (2)
  3. Aff - Purified (1)
Host
  1. Mouse (3)
  2. Hamster (2)
  3. Rabbit (1)
Images
  1. 1 image (6)
  2. 3+ images (1)
Images: Applications
  1. Flow cytometry (F) (2)
  2. Immunoprecipitation (IP) (2)
  3. Western blot / Immunoblot (WB) (2)
  4. ELISA (capture) (E(capture)) (1)
  5. IHC (1)
  6. Paraffin Sections (P) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (10)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn