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Laforin antibody

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Principal name

Laforin antibody

Alternative names for Laforin antibody

EPM2A, Laforin, EC=3.1.3.48, EC=3.1.3.16, Lafora PTPase, LAFPTPase

SwissProt ID

O95278 (Human)

Gene ID

7957 (EPM2A)

Ncbi ID

9606, NP_001018051.1, NP_001018051, NP_005661, NM_005670, NM_001018041

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Ms (Mouse), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Laforin antibody

Kit Component:
- KN209743G1, EPM2A gRNA vector 1 in pCas-Guide vector
- KN209743G2, EPM2A gRNA vector 2 in pCas-Guide vector
- KN209743D, donor vector containing left and right homologous arms and GFP-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

Minassian BA, Lee JR, Herbrick JA, et al. Mutations in a gene encoding a novel protein tyrosine phosphatase cause progressive myoclonus epilepsy. Nat. Genet. 1998; 20:171-4.
Worby CA, Gentry MS, and Dixon JE. Laforin, a dual specificity phosphatase that dephosphorylates complex carbohydrates. J. Biol. Chem. 2006; 281:30412-8.

H00007957-M02 - Laforin
EPM2A monoclonal antibody (M02), clone 6C6 Western Blot analysis of EPM2A expression in PC-12 ( Cat # L012V1 ).
H00007957-M02 - Laforin
Western Blot analysis of EPM2A expression in transfected 293T cell line by EPM2A monoclonal antibody (M02), clone 6C6.Lane 1: EPM2A transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.
H00007957-M02 - Laforin
Detection limit for recombinant GST tagged EPM2A is approximately 1ng/ml as a capture antibody.
PAB3106 - Laforin
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with EPM2A polyclonal antibody ( Cat # PAB3106 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PAB3106 - Laforin
Western blot analysis of EPM2A ( arrow ) using rabbit EPM2A polyclonal antibody ( Cat # PAB3106 ) . 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the EPM2A gene ( Lane 2 ) ( Origene Technologies ) .
H00007957-M01 - Laforin
EPM2A monoclonal antibody (M01), clone 4A12. Western Blot analysis of EPM2A expression in PC-12 ( Cat # L012V1 ).
H00007957-M01 - Laforin
Detection limit for recombinant GST tagged EPM2A is approximately 3ng/ml as a capture antibody.
H00007957-D01P - Laforin
Western Blot analysis of EPM2A expression in transfected 293T cell line (H00007957-T02) by EPM2A MaxPab polyclonal antibody.Lane 1: EPM2A transfected lysate(37.20 KDa).Lane 2: Non-transfected lysate.
AM09050PU-N - Laforin
Flow cytometr  analysis of EPM2A in HeLa cell line, staining at 2-5ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
AM09050PU-N - Laforin
Western blot analysis: Cell lysates of HeLa and 293T (20 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EPM2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
AP11437PU-N - Laforin
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with EPM2A antibody (C-term) (AP11437PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
AP11437PU-N - Laforin
Western blot analysis of EPM2A (arrow) using rabbit polyclonal EPM2A Antibody (C-term) (AP11437PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPM2A gene (Lane 2) (Origene Technologies).
MAB2052 - Laforin
Cell lysates of HeLa ( lane 1 ) and 293T ( lane 2 ) ( 20 µg ) were resolved by SDS-PAGE and probed with EPM2A monoclonal antibody, clone k2A3 ( Cat # MAB2052 ) ( 1:1000 ). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
PAB11469 - Laforin
EPM2A polyclonal antibody ( Cat # PAB11469 ) ( 0.1 µg/ml ) staining of human cerebellum lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence.
H00007957-D01 - Laforin
Immunoprecipitation of EPM2A transfected lysate using anti-EPM2A MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with EPM2A MaxPab mouse polyclonal antibody (B01) (H00007957-B01).
75-222 - Laforin
Immunoblot against crude membrane fractions from whole rat (RBM) or mouse (MBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N84/37 (left) or N52A/42 (right) TC supe.
73-222 - Laforin
Immunoblot against crude membrane fractions from whole rat (RBM) or mouse (MBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N84/37 (left) or N52A/42 (right) TC supe.
TA305859 - Laforin
TA305859 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA319742 - Laforin
Western blot analysis of SW480 in SW480 cell lysate with EPM2A antibody at (A) 1 and (B) 2 ug/mL.
TA319742 - Laforin
Immunocytochemistry of EPM2A in SW480 cells with EPM2A antibody at 2.5 ug/mL.
TA319742 - Laforin
Immunofluorescence of EPM2A in SW480 cells with EPM2A antibody at 5 ug/mL.
TA343303 - Laforin
Host: Rabbit; Target Name: EPM2A; Sample Tissue: THP-1 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml

8 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Laforin (Isoform A) antibody
  Goat Anti-Laforin (isoform a) Antibody  
TA305859 (0.1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

Laforin (C-term) antibody
  Rabbit Polyclonal EPM2A Antibody  
Western blot analysis of SW480 in SW480 cell lysate with EPM2A antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €415.00
  OriGene Technologies, Inc.
+2 additional images

Laforin antibody
Immunoblot against crude membrane fractions from whole rat (RBM) or mouse (MBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N84/37 (left) or N52A/42 (right) TC supe. Mouse IgG1 N84/37 Supernatant Hu ICC/IF, WB
5 ml / €490.00
  Antibodies Incorporated
 

NeuroMab

Laforin antibody
Immunoblot against crude membrane fractions from whole rat (RBM) or mouse (MBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] probed with N84/37 (left) or N52A/42 (right) TC supe. Mouse IgG1 N84/37 Purified Hu ICC/IF, WB
0.1 ml / €570.00
  Antibodies Incorporated
 

NeuroMab

Laforin antibody
Flow cytometr  analysis of EPM2A in HeLa cell line, staining at 2-5ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Mouse IgG1 k2A3 Purified Hu E, F, WB
0.1 ml / €330.00
  OriGene Technologies GmbH
+1 additional image

Laforin antibody
Flow cytometr  analysis of EPM2A in HeLa cell line, staining at 2-5ug for 1x106 cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Mouse IgG1 k2A3 Purified Hu E, F, WB
50 µl / €220.00
  OriGene Technologies GmbH
+1 additional image

Laforin (C-term) antibody
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with EPM2A antibody (C-term) (AP11437PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu P, WB
0.4 ml / €415.00
  OriGene Technologies GmbH
+1 additional image

Lysates

Catalog No.    

EPM2A overexpression lysate
EPM2A overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

8 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (7)
Clonality
  1. Monoclonal (4)
  2. Polyclonal (3)
Target
  1. Laforin (7)
Property
  1. C-term (2)
  2. Isoform A (1)
Reactivity
  1. Human (Hu) (7)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (2)
  4. Bovine (Bov) (1)
  5. Canine (Can) (1)
Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Enzyme Immunoassay (E) (4)
  3. Flow cytometry (F) (2)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (2)
  5. Paraffin Sections (P) (2)
Presentation
  1. Purified (4)
  2. Aff - Purified (2)
  3. Supernatant (1)
Host
  1. Mouse (4)
  2. Rabbit (2)
  3. Goat (1)
Images
  1. 1 image (7)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Flow cytometry (F) (2)
  3. Immunofluorescence (IF) (1)
  4. Paraffin Sections (P) (1)
Manufacturer
  1. OriGene Technologies (6)
  2. Antibodies Incorporated (2)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
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