LASP1 antibody
Principal name
LASP1 antibody
Alternative names for LASP1 antibody
LASP-1, MLN50, LIM and SH3 domain protein 1
SwissProt ID
O77506 (Rabit), P80171 (Pig), Q14847 (Human), Q3B7M5 (Bovin), Q61792 (Mouse), Q99MZ8 (Rat)
Ncbi ID
NP_006139.1, 9606, NP_006139, NP_034818.1, NM_010688, NM_032613
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of LASP1 antibody
- Kit Component:
- - KN309144G1, Lasp1 gRNA vector 1 in pCas-Guide vector
- KN309144G2, Lasp1 gRNA vector 2 in pCas-Guide vector
- KN309144D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Schreiber, V., Masson, R., Linares, J. L., Mattei, M. G., Tomasetto, C., Rio, M. C.Chromosomal
assignment and expression pattern of the murine Lasp-1 gene.Gene 207: 171-175, 1998.
2. Tomasetto, C., Moog-Lutz, C., Regnier, C. H., Schreiber, V., Basset, P., Rio, M.-C.Lasp-1 (MLN 50)
defines a new LIM protein subfamily characterized by the association of LIM and SH3
domains.FEBS Lett. 373: 245-249, 1995.
3. Tomasetto, C., Regnier, C., Moog-Lutz, C., Mattei, M. G., Chenard, M. P., Lidereau, R., Basset, P.,
Rio, M. C.Identification of four novel human genes amplified and overexpressed in breast
carcinoma and localized to the q11-q21.3 region of chromosome 17.Genomics 28: 367-376, 1995.
LASP1 polyclonal antibody ( Cat # PAB6082 ) staining ( 0.1 µg/ml ) of human brain ( cerebellum ) extracts ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
LASP1 polyclonal antibody ( Cat # PAB7399 ) ( 0.1 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of LASP1 expression in transfected 293T cell line by LASP1 monoclonal antibody (M05), clone 4F5.Lane 1: LASP1 transfected lysate(29.7 KDa).Lane 2: Non-transfected lysate.
Human MCF-7; WB Suggested Anti-LASP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: MCF7 cell lysate; LASP1 antibody - middle region (ARP54797_P050) in Human MCF-7 cells using Western Blot
Human HepG2; WB Suggested Anti-LASP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate. LASP1 is supported by BioGPS gene expression data to be expressed in HepG2.; LASP1 antibody - C-terminal region (ARP54798_P050) in Human HepG2 cells using Western Blot
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Antibody (0.1 µg/ml) staining of Human Kidney lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western blot analysis of LASP1 Antibody (Center) Cat.-No AP52446PU-N in A549 cell line lysates (35ug/lane). This demonstrates the LASP1 antibody detected the LASP1 protein (arrow).
TA302746 staining (0.1µg/ml) of Human Brain (Cerebellum) extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
TA303301 (0.1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-LASP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: MCF7 cell lysate
WB Suggested Anti-LASP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: HepG2 cell lysateLASP1 is supported by BioGPS gene expression data to be expressed in HepG2
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA302746 | LASP1 (N-term) antibody |
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| Goat Polyclonal Antibody against LASP1 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Por, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| TA303301 | LASP1 antibody |
||||||
| Goat Polyclonal Antibody against LASP1 (Internal Region) | |||||||
 |
Goat | Aff - Purified | Can, Hu | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
| AP23573PU-N | LASP1 (141-153) antibody |
||||||
 |
Goat | Aff - Purified | Elephant, Hu | E, P, WB |
50 µg /
€425.00
|
||
| OriGene Technologies GmbH | |||||||
 +2 additional images |
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| AP52446PU-N | LASP1 (Center) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP319975 | LASP1 |
||||
| Recombinant protein of human LIM and SH3 protein 1 (LASP1) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR203460L2V | Lenti ORF particles, Lasp1 (GFP-tagged) - Mouse LIM and SH3 protein 1 (Lasp1), 200 uL, >10^7 TU/mL |
|
 |
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| MR203460L1V | Lenti ORF particles, Lasp1 (Myc-DDK-tagged) - Mouse LIM and SH3 protein 1 (Lasp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR208215L2V | Lenti ORF particles, Lasp1 (GFP-tagged ORF) - Rat LIM and SH3 protein 1 (Lasp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€930.00
|
|
| OriGene Technologies, Inc. | ||
| RR208215L1V | Lenti ORF particles, Lasp1 (Myc-DDK-tagged ORF) - Rat LIM and SH3 protein 1 (Lasp1), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€830.00
|
|
| OriGene Technologies, Inc. | ||