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LAT antibody

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Principal name

LAT antibody

Alternative names for LAT antibody

pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1

SwissProt ID

O43561 (Human), O54957 (Mouse), O70601 (Rat)

Gene ID

27040 (LAT), 81511 (Lat), 16797

Ncbi ID

NP_001014987.1, NP_001014987, 9606, 10116, NM_001014987, NP_034819.1, NM_010689, NM_014387, NM_001014988

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Flow Cytometry (F)

Background of LAT antibody

This LAT.10-17 (10-17) monoclol antibody reacts with human Linker for Activation of T cells (LAT), which is essential for T cell receptor (TCR)-mediated sigl transduction. Characterized as an integral transmembrane protein, this ~37-kDa molecule does not possess a true extracellular domain. Upon TCR/CD3 engagement, LAT localizes to lipid rafts and is tyrosine phosphorylated by ZAP-70, leading to the recruitment and activation of other downstream sigling molecules such as Grb-2, PLCγ, and PI3K. This adapter molecule has been shown to be required for T cell activation, as well as thymocyte and T regulatory cell development. In humans, LAT is expressed on peripheral blood lymphocytes, lymph nodes, and tonsil. Studies in mice have shown that LAT is expressed in thymocytes, T cells, mast cells, tural killer cells, megakaryocytes, platelets, and early B cells. This antibody does not crossreact with the mouse antigen.

General readings

Paz PE., et al. (2001) Biochem J. 356(2):461-71.Zhang W., et al. (1998) Cell. 92(1):83-92.

A0504-1 - LAT
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using LAT (Phospho-Tyr191) Antibody. The picture on the right is treated with the synthesized peptide.
A0504-1 - LAT
Western blot analysis of extracts from NIH-3T3 cells, using LAT (Phospho-Tyr191) Antibody. The lane on the right is treated with the synthesized peptide.
A0669-1 - LAT
Western blot analysis of extracts from Jurkat cells treated with UV 15', using LAT (Phospho-Tyr171) Antibody. The lane on the right is treated with the synthesized peptide.
AM03086PU-N - LAT
Figure 1B. Distribution of LAT and NTAL on rat basophilic leukemia cells.
AM03086PU-N - LAT
Figure 1A. Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets. LAT and NTAL were detected on mouse bone marrow-derived mast cells (BMMC; Figure 1A) and RBL rat basophilic leukemia cell line Figure 1B by anti-LAT (LAT-1D1) and polyclonal anti-NTAL antibody (AP03007PU-N), followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.
AM20572PU-N - LAT
IHC Image of AM20572PU-NHuman Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20572PU-N - LAT
IHC Image of AM20572PU-NHuman Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
AM20572PU-N - LAT
Western Blot of 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 µg/mL anti-human LAT antibody (AM20572PU-N). Bands were visualized using HRP-conjugated rat anti-mouse IgG.
AP06532PU-N - LAT
Western blot (WB) analysis of LAT antibody (Cat.-No.: AP06532PU-N) in extracts from A549 cells.
AP06533PU-N - LAT
Western blot (WB) analysis of LAT antibody (Cat.-No.: AP06533PU-N) in extracts from NIH/3T3 cells treated with 293 UV 5'.
AP21126PU-N - LAT
Western blot (WB) analysis of LAT antibody (Cat.-No.: AP21126PU-N) in extracts from Jurkat cells.
B0504-1 - LAT
Western blot analysis of extracts from NIH-3T3 cells, using LAT (Ab-191) Antibody. The lane on the right is treated with the synthesized peptide.
B0669-1 - LAT
Western blot analysis of extracts from A549 cells, using LAT (Ab-171) Antibody. The lane on the right is treated with the synthesized peptide.
B8208-1 - LAT
Western blot analysis of extracts from Jurkat cells, using LAT (Ab-161) Antibody. The lane on the right is treated with the synthesized peptide.
H00027040-M01 - LAT
Immunoperoxidase of monoclonal antibody to LAT on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml]
H00027040-M01 - LAT
Detection limit for recombinant GST tagged LAT is approximately 0.03ng/ml as a capture antibody.
MAB3623 - LAT
Distribution of Lat and NTAL in separate clusters on mast cell plasma membrane sheets.Lat and NTAL were detected on mouse bone marrow-derived mast cells ( BMMC ; A ) and RBL rat basophilic leukemia cell line ( B ) by Lat monoclonal antibody, clone LAT-1D1 ( Cat # MAB3623 ) and polyclonal anti-NTAL antibody, followed by 5 or 10 nm gold-conjugated secondary antibodies, respectively.
MAB3865 - LAT
Flow Cytometry analysis of LAT expression in human peripheral blood lymphocytes ( PBL ) using LAT monoclonal antibody, clone LAT-01 ( Cat # MAB3865 ) (surface markers were stained prior to intracellular staining of human LAT).Panel A : gate on CD4+ cells.Panel B : gate on CD8+ cells.
PAB6652 - LAT
LAT polyclonal antibody ( Cat # PAB6652 ) ( 0.01 µg/ml ) staining of peripheral blood mononucleocytes lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
SM3119P - LAT
Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (LAT-01) (surface markers were stained prior to intracellular staining of human LAT). Panel A: gate on CD4+ cells. Panel B: gate on CD8+ cells.
AR50063PU-N - LAT
AM33107PU-N - LAT
Whole cell extract from human peripheral blood mononuclear cells was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-LAT Antibody Cat.-No AM33107PU (Clone LAT1111). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
AM33107PU-N - LAT
Formalin-Fixed Paraffin-Embedded Human Thymus stained with LAT Antibody Cat.-No AM33107PU (Clone LAT1111) at 10 µg/ml after heat-induced antigen retrieval.
AM32695PU-S - LAT
Western blot analysis of extracts from Jurkat cells (Lane 1) and Mouse thymocytes (Lane 2) using anti-LAT Antibody Clone 11B.12.
CPA2621-100ul - LAT
Western blot analysis of LAT (pY191) expression in HeLa PMA-treated (A); Jurkat CD3-treated (B); Raw264.7 PMA-treated (C); PC12 PMA-treated (D) whole cell lysates.
CPA3055-100ul - LAT
Western blot analysis of LAT (pY255) expression in HEK293T (A); Raw264.7 (B); H9C2 (C) whole cell lysates.
TA320411 - LAT
Immunoblotting of 10e6 normal peripheral human blood cells (lane 1), Jurkat (lane 2), and C57BL/6 splenocytes (lane 3) with 5 ug/mL Anti-Human LAT Purified. Bands were visualized using Anti-Mouse IgG HRP.
TA326210 - LAT
Western blot analysis of extracts from 293 cells, using LAT (Ab-255) Antibody.The lane on the right is treated with the synthesized peptide.
TA327925 - LAT
Western blot analysis of extracts from Jurkat cells (lane 1) and mouse thymocytes (lane 2) using anti-LAT, clone 11B.12.
TA332815 - LAT
Western blot analysis of extracts of Jurkat cell line, using LAT antibody.
TA302672 - LAT
TA302672 (0.01µg/ml) staining of peripheral blood mononucleocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LAT antibody
Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (LAT-01) (surface markers were stained prior to intracellular staining of human LAT). Panel A: gate on CD4+ cells. Panel B: gate on CD8+ cells. Mouse IgG1 LAT-01 Purified Hu F, IP, WB
0.1 mg / €240.00
  OriGene Technologies GmbH

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