Latexin antibody
Principal name
Latexin antibody
Alternative names for Latexin antibody
LXN, Latexin, Endogenous carboxypeptidase inhibitor, ECI, Tissue carboxypeptidase inhibitor, TCI, MUM
Ncbi ID
9606, NP_064554, NP_064554.2, NP_058033, NP_064554.3, NM_020169, NP_058033.2, NM_016753, NM_031655
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Latexin antibody
- Kit Component:
- - KN309568G1, Lxn gRNA vector 1 in pCas-Guide vector
- KN309568G2, Lxn gRNA vector 2 in pCas-Guide vector
- KN309568D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
1. Kadouchi I, Sakamoto K, Tangjiao L, Murakami T, Kobayashi E, Hoshino Y, Yamaguchi A. Latexin is involved in bone morphogenetic protein-2-induced chondrocyte differentiation. Biochem Biophys Res Commun. 2009 Jan 16;378(3):600-4. PMID: 19059214
2. Mitsunaga K, Kikuchi J, Wada T, Furukawa Y. Latexin regulates the abundance of multiple cellular proteins in hematopoietic stem cells. J Cell Physiol. 2011 May 12.
PMID: 21567403
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with LXN polyclonal antibody ( Cat # PAB3125 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Latexin ( arrow ) using LXN polyclonal antibody ( Cat # PAB3125 ). 293 cell lysates ( 2 µg/lane ) either nontransfected ( Lane 1 ) or transiently transfected with the LXN gene ( Lane 2 ) ( Origene Technologies ).
LXN polyclonal antibody ( Cat # PAB7228 ) ( 0.1 µg/ml ) staining of rat brain lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot analysis of LXN expression in transfected 293T cell line (H00056925-T01) by LXN MaxPab polyclonal antibody.Lane 1: LXN transfected lysate(24.42 KDa).Lane 2: Non-transfected lysate.
Recombinant human LXN, 1-222 aa: 15% SDS-PAGE (3 µg)
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Latexin antibody (Center) (AP11459PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of Latexin (arrow) using rabbit polyclonal Latexin Antibody (Center) (AP11459PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2) (Origene Technologies).
Human Spleen (formalin-fixed, paraffin-embedded) stained with LXN antibody AP22768PU-N followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot analysis of Latexin (arrow) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the LXN gene (Lane 2)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503644)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503644)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503644)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503644)
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503644)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXN (RC202769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LXN monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LXN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503779)
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-LXN monoclonal antibody.(1:200)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXN (RC202769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN.
HEK293T cells transfected with either RC202769 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LXN antibody(TA503779), and then analyzed by flow cytometry.
TA303204 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: A549 cells, Primary antibody: (LXN Antibody) at dilution 1/667, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (LXN Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using (LXN Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA503644 | Latexin antibody |
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| LXN mouse monoclonal antibody, clone OTI2G4 (formerly 2G4) | |||||||
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Mouse | IgG1 | OTI2G4 | Purified | Hu, Ms | P, WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody!
|
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| TA503779 | Latexin antibody |
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| LXN mouse monoclonal antibody, clone OTI1E10 (formerly 1E10) | |||||||
 |
Mouse | IgG1 | OTI1E10 | Purified | Hu | F, P, WB |
0.1 ml /
€360.00
|
| OriGene Technologies, Inc. | |||||||
| +10 additional images |
Highly validated antibody!
|
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| TA303204 | Latexin (C-term) antibody |
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| Goat Polyclonal Antibody against LXN | |||||||
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Goat | Aff - Purified | Can, Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€360.00
|
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| OriGene Technologies, Inc. | |||||||
| TA349384 | Latexin antibody |
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| Rabbit Polyclonal Anti-LXN Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| AP11459PU-N | Latexin (Center) antibody |
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Rabbit | Ig | Aff - Purified | Hu | P, WB |
0.4 ml /
€415.00
|
|
| OriGene Technologies GmbH | |||||||
| +1 additional image |
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| AP22768PU-N | Latexin (78-107) antibody |
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Rabbit | Purified | Hu | F, P, WB |
0.2 ml /
€425.00
|
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| OriGene Technologies GmbH | |||||||
| +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP302769 | Latexin |
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| Recombinant protein of human latexin (LXN) | |||||
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Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY412596 | LXN overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
Rat Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RR203261L2V | Lenti ORF particles, Lxn (GFP-tagged ORF) - Rat latexin (Lxn), 200 uL, >10^7 TU/mL |
|
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200 µl /
€930.00
|
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| OriGene Technologies, Inc. | ||
| RR203261L1V | Lenti ORF particles, Lxn (Myc-DDK-tagged ORF) - Rat latexin (Lxn), 200 uL, >10^7 TU/mL |
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200 µl /
€830.00
|
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| OriGene Technologies, Inc. | ||