Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Target » Layilin antibody

Layilin antibody

(reset)

Principal name

Layilin antibody

Alternative names for Layilin antibody

LAYN

SwissProt ID

Q6UX15 (Human)

Gene ID

143903 (LAYN)

Ncbi ID

9606, NP_849156, NP_001245319, NP_849156.1, NM_178834

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Flow Cytometry (F)

Background of Layilin antibody

Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.

General readings

Bono P., et al. (2005) Exp Cell Res 1;308(1): 177-87Mark L., et al. (1998) The Journal of Cell Biology 143(2): 429

ARP53385_P050 - Layilin
Human MCF-7; WB Suggested Anti-LAYN Antibody Titration: 0.2-1 ug/ml. Positive Control: MCF7 cell lysate; LAYN antibody - N-terminal region (ARP53385_P050) in Human MCF-7 cells using Western Blot
H00143903-B01P - Layilin
LAYN MaxPab polyclonal antibody. Western Blot analysis of LAYN expression in human liver.
H00143903-B01P - Layilin
Western Blot analysis of LAYN expression in transfected 293T cell line (H00143903-T02) by LAYN MaxPab polyclonal antibody.Lane 1: LAYN transfected lysate(41.14 KDa).Lane 2: Non-transfected lysate.
AR51070PU-N - Layilin
AM50080PU-N - Layilin
The cell lysates of A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
TA504450 - Layilin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAYN (RC206197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAYN.
TA504450 - Layilin
HEK293T cells transfected with either RC206197 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LAYN antibody(TA504450), and then analyzed by flow cytometry.
TA504643 - Layilin
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAYN (RC206197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAYN.
TP306197 - Layilin
TA337582 - Layilin
WB Suggested Anti-LAYN Antibody Titration: 0.2-1 ug/ml; Positive Control: MCF7 cell lysate

6 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Layilin antibody
  LAYN mouse monoclonal antibody, clone OTI4C11 (formerly 4C11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAYN (RC206197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAYN. Mouse IgG1 OTI4C11 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.
+1 additional image

TrueMAB

Layilin antibody
  LAYN mouse monoclonal antibody, clone OTI4D3 (formerly 4D3)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LAYN (RC206197, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LAYN. Mouse IgG1 OTI4D3 Purified Hu WB
0.1 ml / €360.00
  OriGene Technologies, Inc.
 

TrueMAB

Layilin antibody
The cell lysates of A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT20G8 Purified Hu E, WB
0.1 ml / €330.00
  OriGene Technologies GmbH

Layilin antibody
The cell lysates of A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LAYN antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG2b AT20G8 Purified Hu E, WB
50 µl / €220.00
  OriGene Technologies GmbH

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Layilin
  Recombinant protein of human layilin (LAYN)  
Layilin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.
 

Lysates

Catalog No.    

LAYN overexpression lysate
LAYN overexpression lysate  
0.1 mg / €295.00
  OriGene Technologies, Inc.

6 Item(s)

per page

Available Filters

Product Type
  1. Lysate (1)
  2. Primary Antibody (4)
  3. Protein | Growth Factor (1)
Clonality
  1. Monoclonal (4)
Target
  1. Layilin (5)
Reactivity
  1. Human (Hu) (4)
Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Enzyme Immunoassay (E) (2)
  3. Flow cytometry (F) (1)
Presentation
  1. Purified (4)
Host
  1. Mouse (4)
Images
  1. 1 image (5)
Images: Applications
  1. Western blot / Immunoblot (WB) (4)
  2. Flow cytometry (F) (1)
Species (Protein)
  1. Human (1)
Source
  1. HEK293 cells (1)
Manufacturer
  1. OriGene Technologies (6)
Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn