LDB1 antibody
Principal name
LDB1 antibody
Alternative names for LDB1 antibody
LDB1, CLIM2, LIM domain-binding protein 1, Nuclear LIM interactor, Carboxyl-terminal LIM domain-binding protein 2, CLIM-2, LIM domain-binding factor CLIM2, hLdb1
Ncbi ID
NP_003884, 9606, 10090, NP_001106878, NM_003893, NP_001106878.1, NP_001106879.1, NM_010697, NM_001113408, NM_001113407
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)
Background of LDB1 antibody
- Kit Component:
- - KN309196G1, Ldb1 gRNA vector 1 in pCas-Guide vector
- KN309196G2, Ldb1 gRNA vector 2 in pCas-Guide vector
- KN309196D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Howard, P.W., et al. Biochem. Biophys. Res. Commun. 396(3):674-678(2010)
Howard, P.W., et al. Biochem. J. 429(1):127-136(2010)
Bhati, M., et al. EMBO J. 27(14):2018-2029(2008)
Ryan, D.P., et al. Proteins 70(4):1461-1474(2008)
Tran, Y.H., et al. J. Biochem. 140(1):105-119(2006)
Human Jurkat; WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate. LDB1 is supported by BioGPS gene expression data to be expressed in Jurkat.; LDB1 antibody - middle region (ARP37731_P050) in Human Jurkat cells using Western Blot
LDB1 monoclonal antibody (M02), clone 2G9 Western Blot analysis of LDB1 expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged LDB1 is approximately 1ng/ml as a capture antibody.
LDB1 monoclonal antibody (M03), clone 4G8 Western Blot analysis of LDB1 expression in IMR-32 ( Cat # L008V1 ).
Detection limit for recombinant GST tagged LDB1 is approximately 0.3ng/ml as a capture antibody.
Human HepG2; WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; LDB1 antibody - C-terminal region (ARP37730_P050) in Human HepG2 cells using Western Blot
Western blot using Ldb1 polyclonal antibody ( Cat # PAB10257 ) shows detection of Ldb1 protein ( arrowhead ) in Jurkat whole cell lysate.Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose.Primary antibody was used at a 1 : 1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.The membrane was washed and reacted with a 1:20,000 dilution of DyLightTM800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature ( 800 nm channel, green ).Molecular weight estimation was made by comparison to prestained MW markers in lane M ( 700 nm channel, red ).Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
Western blot analysis of LDB1 Antibody (C-term) Cat.-No AP52458PU-N in ZR-75-1 cell line lysates (35ug/lane). This demonstrates the LDB1 antibody detected the LDB1 protein (arrow).
LDB1 antibody [N2C3] detects LDB1 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 10 % SDS-PAGE. LDB1 antibody [N2C3] (TA308742) dil
Sample (50 ug of whole cell lysate). A: Mouse brain. 10% SDS PAGE. TA308742 diluted at 1:1000.
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. LDB1 antibody [N2C3] (TA308742) dilution: 1:500.
LDB1 antibody [N2C3] detects LDB1 protein at nucleus on rat middle brain by immunohistochemical analysis. Sample: Paraffin-embedded rat middle brain. LDB1 antibody [N2C3] (TA308742) dilution: 1:500.
Immunohistochemical analysis of paraffin-embedded U87 Xenograft, using LDB1(TA308742) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using LDB1(TA308742) antibody at 1:200 dilution.
LDB1antibody immunoprecipitates LDB1 protein in IP experiments. IP Sample: 1000 ug 293T whole cell lysate/extract. A. 20 ug 293T whole cell lysate/extract. B. Control with 2.5 ug of preimmune rabbit IgG. C. Immunoprecipitation of LDB1 protein by 2.5 ug of
WB using Anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate
WB Suggested Anti-LDB1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysateLDB1 is supported by BioGPS gene expression data to be expressed in Jurkat
- Quick-Filters:
- Highly Validated (1)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA308742 | LDB1 antibody |
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| Rabbit Polyclonal antibody to LDB1 (LIM domain binding 1) | |||||||
![LDB1 antibody [N2C3] detects LDB1 protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug A431 whole cell extract. C. 30 ug HeLa whole cell extract. D. 30 ug HepG2 whole cell extract. 10 % SDS-PAGE. LDB1 antibody [N2C3] (TA308742) dil](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/GTX104531-1-W.jpg) |
Rabbit | IgG | Aff - Purified | Hu, Ms | ICC/IF, IP, P, WB |
0.1 ml /
€415.00
|
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| TA319355 | LDB1 antibody |
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| Rabbit polyclonal anti-LDB1 antibody | |||||||
![WB using Anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate. Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose. Primary antibody was used at a 1:1,800 dilution. The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/OriGene/600-401-350-1-W.jpg) |
Rabbit | Aff - Purified | Chk, Hu, Ms, Xen | E |
0.1 mg /
€515.00
|
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| OriGene Technologies, Inc. | |||||||
| AP52458PU-N | LDB1 (C-term) antibody |
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Rabbit | Ig | Aff - Purified | Hu | WB |
0.4 ml /
€415.00
|
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| OriGene Technologies GmbH | |||||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY418367 | LDB1 overexpression lysate |
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0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |