LDB3 antibody
Principal name
LDB3 antibody
Alternative names for LDB3 antibody
KIAA0613, ZASP, LIM domain-binding protein 3
Ncbi ID
9606, NP_001073584, NP_001073585.1, NM_001080115, NM_001080116, NP_001073585, NP_001034160.1, NM_001039071, NM_001039074, NM_001039072, NM_011918, NM_001039073, NM_001039076, NM_001080114, NM_007078, NM_001171611, NM_001171610, NP_001073583.1
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))
Background of LDB3 antibody
- Kit Component:
- - KN309198G1, Ldb3 gRNA vector 1 in pCas-Guide vector
- KN309198G2, Ldb3 gRNA vector 2 in pCas-Guide vector
- KN309198D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Selcen D, Engel AG. Mutations in ZASP define a novel form of muscular dystrophy in humans. Ann Neurol. 2005 Feb;57(2):269-76.
Immunofluorescence of monoclonal antibody to LDB3 on A-431 cell . [antibody concentration 10 ug/ml]
Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi ( Cat # H00011155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with LDB3 monoclonal antibody (M06), clone 3C8 (Cat # H00011155-M06 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
LDB3 monoclonal antibody (M06), clone 3C8 Western Blot analysis of LDB3 expression in A-431 ( Cat # L015V1 ).
Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody (M06), clone 3C8.Lane 1: LDB3 transfected lysate(31 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged LDB3 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence of purified MaxPab antibody to LDB3 on HeLa cell. [antibody concentration 10 ug/ml]
LDB3 MaxPab polyclonal antibody. Western Blot analysis of LDB3 expression in human colon.
Western Blot analysis of LDB3 expression in transfected 293T cell line (H00011155-T01) by LDB3 MaxPab polyclonal antibody.Lane 1: LDB3 transfected lysate(31.13 KDa).Lane 2: Non-transfected lysate.
LDB3 polyclonal antibody ( Cat # PAB6864 ) ( 0.01 µg/ml ) staining of human muscle lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence.
LDB3 monoclonal antibody (M06A), clone 3C8 Western Blot analysis of LDB3 expression in A-431 ( Cat # L015V1 ).
Detection limit for recombinant GST tagged LDB3 is approximately 0.03ng/ml as a capture antibody.
Human Heart; WB Suggested Anti-LDB3 Antibody Titration: 0.625ug/ml. Positive Control: Human heart; LDB3 antibody - N-terminal region (ARP41588_T100) in Human Heart cells using Western Blot
LDB3 monoclonal antibody (M04), clone 2C1. Western Blot analysis of LDB3 expression in A-431(Cat # L015V1 ).
TA303056 (0.01µg/ml) staining of human muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
WB Suggested Anti-LDB3 Antibody Titration: 0.625 ug/ml; Positive Control: Human heart
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA303056 | LDB3 antibody |
||||||
| Goat Polyclonal Antibody against LDB3 | |||||||
 |
Goat | Aff - Purified | Can, Hu, Ms, Rt | WB |
0.1 mg /
€360.00
|
||
| OriGene Technologies, Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP323117 | LDB3 (transcript variant 3) |
||||
| Purified recombinant protein of Homo sapiens LIM domain binding 3 (LDB3), transcript variant 3 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
| TP323167 | LDB3 (transcript variant 4) |
||||
| Recombinant protein of human LIM domain binding 3 (LDB3), transcript variant 4 | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|
| OriGene Technologies, Inc. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| LY421586 | LDB3 overexpression lysate |
|
 |
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. | ||
| LY421587 | LDB3 overexpression lysate |
|
|
0.1 mg /
€295.00
|
|
| OriGene Technologies, Inc. |
