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LEFTY2 antibody

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Principal name

LEFTY2 antibody

Alternative names for LEFTY2 antibody

LEFTY2, EBAF, LEFTA, LEFTYA, TGFB4, Left-right determination factor 2, Protein lefty-2, Left-right determination factor A, Protein lefty-A, Transforming growth factor beta-4, TGF-beta-4, Endometrial bleeding-associated factor

SwissProt ID

O00292 (Human), P57785 (Mouse)

Gene ID

7044 (LEFTY2), 320202 (Lefty2)

Ncbi ID

9606, NP_003231, NP_003231.2, NP_796073.1, NM_177099, NM_003240, NM_001172425

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Can (Canine), Eq (Equine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)

Background of LEFTY2 antibody

During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 Kda) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28kDa form induces MAPK activity.

General readings

Cornet,P.B., (2005) J. Clin. Endocrinol. Metab. 90 (2), 1001-1011

H00007044-M04 - LEFTY2
LEFTY2 monoclonal antibody (M04), clone 1H6. Western Blot analysis of LEFTY2 expression in human Skeletal muscle.
H00007044-B01 - LEFTY2
Western Blot analysis of LEFTY2 expression in transfected 293T cell line (H00007044-T01) by LEFTY2 MaxPab polyclonal antibody.Lane 1: LEFTY2 transfected lysate(40.26 KDa).Lane 2: Non-transfected lysate.
ARP42107_P050 - LEFTY2
Human Uterus; Immunohistochemistry with Uterus tissue at an antibody concentration of 5ug/ml using anti-LEFTY2 antibody (ARP42107_P050); LEFTY2 antibody - N-terminal region (ARP42107_P050) in Human Uterus cells using Immunohistochemistry
ARP42107_P050 - LEFTY2
Human RPMI 8226; WB Suggested Anti-LEFTY2 Antibody Titration: 0.2-1 ug/ml. Positive Control: RPMI 8226 cell lysate. LEFTY2 is supported by BioGPS gene expression data to be expressed in RPMI 8226.; LEFTY2 antibody - N-terminal region (ARP42107_P050) in Human RPMI 8226 cells using Western Blot
MAB2032 - LEFTY2
LEFTY2 monoclonal antibody, clone 7C5G1H6H10 ( Cat # MAB2032 ) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY2.Detection occurs after 1.0 µg of protein is loaded in each lane.The blot was incubated with a 1:2,000 dilution of LEFTY2 monoclonal antibody, clone 7C5G1H6H10 at room temperature for 30 min followed by detection using IRDyeTM800 labeled Goat-a-Mouse IgG [H&L] diluted 1 : 1,000.Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag.Lane 2 contains partially purified recombinant human LEFTY.The antibody may be used to detect endogenous human LEFTY.
H00007044-D01P - LEFTY2
Western Blot analysis of LEFTY2 expression in transfected 293T cell line (H00007044-T01) by LEFTY2 MaxPab polyclonal antibody.Lane 1: LEFTY2 transfected lysate(40.90 KDa).Lane 2: Non-transfected lysate.
H00007044-D01 - LEFTY2
Immunoprecipitation of LEFTY2 transfected lysate using anti-LEFTY2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with LEFTY2 MaxPab mouse polyclonal antibody (B01) (H00007044-B01).
TA308258 - LEFTY2
Sample (30 ug of whole cell lysate). A: A431 . 10% SDS PAGE. TA308258 diluted at 1:1000.
TA308258 - LEFTY2
Immunohistochemical analysis of paraffin-embedded Colon ca, using LEFTY2(TA308258) antibody at 1:500 dilution.
TA319606 - LEFTY2
Mab anti-Human LEFTY antibody to detect by WB partially purified recombinant 6X His tagged human LEFTY. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at RT for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY.
TA350490 - LEFTY2
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (LEFTY2 Antibody) at dilution 1/800 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 30 seconds
TA350490 - LEFTY2
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (LEFTY2 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
TA350490 - LEFTY2
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (LEFTY2 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)
TA346347 - LEFTY2
Host: Rabbit; Target Name: LEFTY2; Sample Tissue: Thymus Tumor lysates; Antibody Dilution: 1.0 ug/ml
TA346346 - LEFTY2
WB Suggested Anti-LEFTY2 Antibody Titration: 0.2-1 ug/ml; Positive Control: RPMI 8226 cell lysate.LEFTY2 is supported by BioGPS gene expression data to be expressed in RPMI 8226
TA346346 - LEFTY2
Immunohistochemistry with Uterus tissue at an antibody concentration of 5 ug/ml using anti-LEFTY2 antibody

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

LEFTY2 antibody
  Rabbit polyclonal antibody to Lefty -A (left-right determination factor 2)  
Sample (30 ug of whole cell lysate). A: A431 . 10% SDS PAGE. TA308258 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €415.00
  OriGene Technologies, Inc.
+1 additional image

LEFTY2 antibody
  Mouse monoclonal LEFTY A antibody  
Mab anti-Human LEFTY antibody to detect by WB partially purified recombinant 6X His tagged human LEFTY. The blot was incubated with a 1:2,000 dilution of Mab anti-Human LEFTY at RT for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] diluted 1:1,000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. Mouse IgG2a 7C5G1H6H10 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €515.00
  OriGene Technologies, Inc.

LEFTY2 antibody
  Rabbit Polyclonal Anti-LEFTY2 Antibody  
Gel: 8%SDS-PAGE,Lysate: 40 ug,,Primary antibody: (LEFTY2 Antibody) at dilution 1/800 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+2 additional images

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Lefty2 (GFP-tagged) - Mouse left-right determination factor 2 (Lefty2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Lefty2 (GFP-tagged) - Mouse left-right determination factor 2 (Lefty2), 200 uL, >10^7 TU/mL  
200 µl / €930.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Lefty2 (Myc-DDK-tagged) - Mouse left-right determination factor 2 (Lefty2), 200 uL, >10^7 TU/mL
Lenti ORF particles, Lefty2 (Myc-DDK-tagged) - Mouse left-right determination factor 2 (Lefty2), 200 uL, >10^7 TU/mL  
200 µl / €830.00
  OriGene Technologies, Inc.
 

5 Item(s)

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Available Filters

Product Type
  1. Mouse Lenti ORF Particle (2)
  2. Primary Antibody (3)
Clonality
  1. Polyclonal (2)
  2. Monoclonal (1)
Target
  1. LEFTY2 (3)
Reactivity
  1. Human (Hu) (3)
  2. Mouse (Ms) (2)
  3. Rat (Rt) (1)
Applications
  1. Paraffin Sections (P) (3)
  2. Western blot / Immunoblot (WB) (3)
  3. Enzyme Immunoassay (E) (2)
Presentation
  1. Purified (2)
  2. Aff - Purified (1)
Host
  1. Rabbit (2)
  2. Mouse (1)
Images
  1. 1 image (3)
  2. 3+ images (1)
Images: Applications
  1. Western blot / Immunoblot (WB) (3)
  2. IHC (2)
Manufacturer
  1. OriGene Technologies (5)
Service
  • OriGene Technologies GmbH
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  • D-32052 Herford
  • Germany
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  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
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  • orders: ordering-de@origene.com
 
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